3.2.1.B35: Thermococcus kodakaraensis beta-glycosidase

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For detailed information about Thermococcus kodakaraensis beta-glycosidase, go to the full flat file.

Reaction

Exo-type beta-glycosidase activity with cellobiose, cellotriose, cellotetraose or cellopentaose. In vitro it shows high catalytic efficiency with 4-nitrophenyl beta-D-glucopyranoside or 4-nitrophenyl beta-D-mannopyranoside, and lower catalytic efficiency with 4-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-xylopyranoside =

Synonyms

Pyrococcus kodakaraensis beta-glycosidase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.B35 Thermococcus kodakaraensis beta-glycosidase

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Results	in table
1	AA Sequence
2	Cloned(Commentary)
10	kcat/KM [mM/s]
10	KM Value [mM]
1	Molecular Weight [Da]
2	Organism
1	pH Optimum
5	Protein Variants
2	Purification (Commentary)
1	Reaction
2	Reference
13	Substrates and Products (Substrate)
2	Subunits
1	Synonyms
3	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
10	Turnover Number [1/s]

Engineering

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Engineering on EC 3.2.1.B35 - Thermococcus kodakaraensis beta-glycosidase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D206N

Thermococcus kodakarensis

Q9YGB8

mutation alters the catalytic turn-over rate for glucosidase and mannosidase activities with fucosidase activity remain unchanged

731734

D206Q

Thermococcus kodakarensis

Q9YGB8

catalytically inactive mutant enzyme. The extended side chain of D206Q is predicted to affect the substrate binding during catalysis

731734

E207S

Thermococcus kodakarensis

Q9YGB8

catalytically inactive mutant enzyme

731734

E399S

Thermococcus kodakarensis

Q9YGB8

catalytically inactive mutant enzyme

731734

Q77R

Thermococcus kodakarensis

Q9YGB8

catalytically inactive mutant enzyme. Q77R might have made some changes in three dimensional structure due to its electrostatic effect and lost its catalytic activity

731734