3.2.1.B33: Sulfolobus shibatae beta-glycosidase

This is an abbreviated version, for detailed information about Sulfolobus shibatae beta-glycosidase, go to the full flat file.

Reaction

Hydrolysis of beta-glycosidic bonds with broad specificity. It prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc) =

Synonyms

Sulfolobus shibatae beta-glycosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.B33 Sulfolobus shibatae beta-glycosidase

Inhibitors

Inhibitors on EC 3.2.1.B33 - Sulfolobus shibatae beta-glycosidase

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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
5 mM, no inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 5% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
4-chloromercuribenzoate
-
5 mM, 94% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 90% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Ba2+
-
1 mM, 5% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 6% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Ca2+
-
1 mM, 5% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 6% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Cd2+
-
no inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 12% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Cu2+
-
1 mM, 81% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 36% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Fe2+
-
1 mM, 1% inhibition of 2-nitrophenyl mbeta-D-galactopyranoside hydrolysis, 16% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Hg2+
-
1 mM, complete inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis and 4-nitrophenyl beta-D-glucopyranoside hydrolysis
monoiodoacetate
-
5 mM, 3% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, no inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Zn2+
-
1 mM, complete inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis and 4-nitrophenyl beta-D-glucopyranoside hydrolysis