3.2.1.94: glucan 1,6-alpha-isomaltosidase

This is an abbreviated version!
For detailed information about glucan 1,6-alpha-isomaltosidase, go to the full flat file.

Reaction

Synonyms

AgIMD, dextranase, isomalto-, exo-isomalto-hydrolase, Exo-isomaltohydrolase, G2-dextranase, Glucan 1,6-alpha-isomaltosidase, IMD, isomalto-dextranase, isomaltodextranase, isomaltohydrolase, exo

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.94 glucan 1,6-alpha-isomaltosidase

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Results	in table
28	AA Sequence
1	CAS Registry Number
8	Cloned(Commentary)
3	Crystallization (Commentary)
17	Inhibitors
8	kcat/KM [mM/s]
2	Ki Value [mM]
9	KM Value [mM]
4	Localization
3	Metals/Ions
5	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
26	Organism
1	Oxidation Stability
8	pH Optimum
3	pH Range
4	pH Stability
24	Protein Variants
9	Purification (Commentary)
3	Reaction
2	Reaction Type
26	Reference
11	Source Tissue
13	Specific Activity [micromol/min/mg]
58	Substrates and Products (Substrate)
1	Subunits
17	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
8	Temperature Stability [°C]
9	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.2.1.94 - glucan 1,6-alpha-isomaltosidase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

4-nitrophenyl alpha-isomaltoside + H2O

4-nitrophenol + alpha-isomaltose

2 entries

alpha-linked gluco-oligosaccharide + H2O

isomaltose + D-glucose

Arthrobacter globiformis

-

-

137021

-

137021

?

dextran + 1-butanol

1-butyl alpha-isomaltoside + H2O

Arthrobacter globiformis

-

-

137024

-

137024

r

dextran + 1-propanol

1-propyl alpha-isomaltoside + H2O

Arthrobacter globiformis

-

-

137024

-

137024

r

dextran + ethanol

ethyl alpha-isomaltoside + H2O

Arthrobacter globiformis

-

-

137024

-

137024

r

dextran + H2O

alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc

8 entries

dextran + methanol

methyl alpha-isomaltoside + H2O

Arthrobacter globiformis

-

-

137024

-

137024

r

dextran + sucrose

6G-alpha-isomaltosylsucrose + H2O

Arthrobacter globiformis

-

-

137024

-

137024

r

dextran + trehalose

6-alpha-isomaltosyltrehalose + H2O

Arthrobacter globiformis

-

-

137024

-

137024

r

dextran T2000 + H2O

isomaltose + ?

2 entries

glucooligosaccharides + H2O

isomaltose + D-glucose

2 entries

isomaltoheptaose + H2O

?

Arthrobacter globiformis

-

-

655809

-

-

?

isomaltohexaose + H2O

?

Arthrobacter globiformis

-

-

655809

-

-

?

isomaltohexatiol + H2O

isomaltose + Isomaltitol

Arthrobacter globiformis

-

-

137021

-

137021

?

isomaltooctaose + H2O

?

Arthrobacter globiformis

-

-

655809

-

-

?

isomaltooligosaccharide + H2O

isomaltose + glucose

2 entries

isomaltopentaose + H2O

?

Arthrobacter globiformis

-

-

655809

-

-

?

isomaltotetraose + H2O

?

Arthrobacter globiformis

-

-

655809

-

-

?

isomaltotriitol + H2O

isomaltose + sorbitol

4 entries

isomaltotriose + H2O

alpha-isomaltose + D-glucose

6 entries

panose + H2O

alpha-isomaltose + D-glucose

6 entries

panose + H2O

isomaltose + D-glucose

4 entries

pullulan + H2O

isopanose

3 entries

additional information

?

-

2 entries

4-nitrophenyl alpha-isomaltoside + H2O

4-nitrophenol + alpha-isomaltose

Arthrobacter globiformis

Q7WSN5

-

751049

-

-

?

4-nitrophenyl alpha-isomaltoside + H2O

4-nitrophenol + alpha-isomaltose

Arthrobacter globiformis T6

Q7WSN5

-

751049

-

-

?

dextran + H2O

alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc

Actinomadura sp.

-

-

137018

-

137018

?

dextran + H2O

alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc

Actinomadura sp. R10

-

-

137018

-

137018

?

dextran + H2O

alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc

Arthrobacter dextranolyticus

Q6BE01

-

654317

-

-

?

dextran + H2O

alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc

Arthrobacter globiformis

-

-

137018

-

137018

?

dextran + H2O

alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc

Arthrobacter globiformis

-

-

137019

-

137019

?

dextran + H2O

alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc

Arthrobacter globiformis

-

-

137021

-

137021

?

dextran + H2O

alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc

Arthrobacter globiformis

-

-

654117, 655108, 664121

-

-

?

dextran + H2O

alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc

Arthrobacter globiformis

-

enzyme hydrolyzes alpha-1,6-glucosidic linkages of dextran or isomalto-oligosaccharides to release exolytically alpha-isomaltose from the non-reducing ends

655812

-

-

?

dextran T2000 + H2O

isomaltose + ?

Arthrobacter globiformis

Q7WSN5

the enzyme liberates isomaltose from the non-reducing end of a polymer of dextran. It does not show alpha-N-galactosidase or alpha-N-acetylgalactosaminidase activities

751049

-

-

?

dextran T2000 + H2O

isomaltose + ?

Arthrobacter globiformis T6

Q7WSN5

the enzyme liberates isomaltose from the non-reducing end of a polymer of dextran. It does not show alpha-N-galactosidase or alpha-N-acetylgalactosaminidase activities

751049

-

-

?

glucooligosaccharides + H2O

isomaltose + D-glucose

Actinomadura sp.

-

-

137018

-

137018

?

glucooligosaccharides + H2O

isomaltose + D-glucose

Arthrobacter globiformis

-

-

137018

-

137018

?

isomaltooligosaccharide + H2O

isomaltose + glucose

Arthrobacter globiformis

-

-

137021

glucose not with isomaltotetraose and isomaltohexaose

137021

r

isomaltooligosaccharide + H2O

isomaltose + glucose

Arthrobacter globiformis

-

condensation of isomaltose to isomaltotetraose

137022

-

-

r

isomaltotriitol + H2O

isomaltose + sorbitol

Actinomadura sp.

-

-

137018

-

-

?

isomaltotriitol + H2O

isomaltose + sorbitol

Actinomadura sp. R10

-

-

137018

-

-

?

isomaltotriitol + H2O

isomaltose + sorbitol

Arthrobacter globiformis

-

-

137018

-

-

?

isomaltotriitol + H2O

isomaltose + sorbitol

Arthrobacter globiformis

-

-

137023

-

137023

?

isomaltotriose + H2O

alpha-isomaltose + D-glucose

Actinomadura sp.

-

-

137018

-

137018

?

isomaltotriose + H2O

alpha-isomaltose + D-glucose

Actinomadura sp. R10

-

-

137018

-

137018

?

isomaltotriose + H2O

alpha-isomaltose + D-glucose

Arthrobacter globiformis

-

-

137017

-

137017

?

isomaltotriose + H2O

alpha-isomaltose + D-glucose

Arthrobacter globiformis

-

-

137018

-

137018

?

isomaltotriose + H2O

alpha-isomaltose + D-glucose

Arthrobacter globiformis

-

-

655809

-

-

?

isomaltotriose + H2O

alpha-isomaltose + D-glucose

Arthrobacter globiformis

-

hydrolyzes alpha-1,6-glucosidic linkage

655808

-

-

?

panose + H2O

alpha-isomaltose + D-glucose

Actinomadura sp.

-

-

137018

-

137018

?

panose + H2O

alpha-isomaltose + D-glucose

Actinomadura sp. R10

-

-

137018

-

137018

?

panose + H2O

alpha-isomaltose + D-glucose

Arthrobacter globiformis

-

-

137017

-

137017

?

panose + H2O

alpha-isomaltose + D-glucose

Arthrobacter globiformis

-

-

137018

-

137018

?

panose + H2O

alpha-isomaltose + D-glucose

Arthrobacter globiformis

-

-

137023

-

-

?

panose + H2O

alpha-isomaltose + D-glucose

Arthrobacter globiformis

-

hydrolyzes alpha-1,4-glucosidic linkage

655808

-

-

?

panose + H2O

isomaltose + D-glucose

Actinomadura sp.

-

-

137018

-

-

?

panose + H2O

isomaltose + D-glucose

Actinomadura sp. R10

-

-

137018

-

-

?

panose + H2O

isomaltose + D-glucose

Arthrobacter globiformis

-

-

137017, 137018

-

-

?

panose + H2O

isomaltose + D-glucose

Arthrobacter globiformis

-

-

137023

-

137023

?

pullulan + H2O

isopanose

Arthrobacter globiformis

-

-

136635

-

136635

?

pullulan + H2O

isopanose

Arthrobacter globiformis

-

enzyme also has a weak isopullulanase activity

655808, 655812

-

-

?

pullulan + H2O

isopanose

Arthrobacter globiformis T6

-

-

136635

-

136635

?

additional information

?

-

Arthrobacter globiformis

-

enzyme also hydrolyzes oligosaccharides with alpha-isomaltosyl unit in their non-reducing ends, no substrates are isopanose and maltotriose

655809

-

-

?

additional information

?

-

Arthrobacter globiformis

-

enzyme is highly specific for the glucosyl moiety in both hydrolysis and transglycosylation

655814

-

-

?