3.2.1.94: glucan 1,6-alpha-isomaltosidase
This is an abbreviated version!
For detailed information about glucan 1,6-alpha-isomaltosidase, go to the full flat file.
Word Map on EC 3.2.1.94
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3.2.1.94
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globiformis
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arthrobacter
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dextrans
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glycoside
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isomaltose
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nonreducing
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starch
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carbohydrate-binding
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sitting-drop
- 3.2.1.94
- globiformis
- arthrobacter
- dextrans
- glycoside
- isomaltose
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nonreducing
- starch
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carbohydrate-binding
-
sitting-drop
Reaction
Synonyms
AgIMD, dextranase, isomalto-, exo-isomalto-hydrolase, Exo-isomaltohydrolase, G2-dextranase, Glucan 1,6-alpha-isomaltosidase, IMD, isomalto-dextranase, isomaltodextranase, isomaltohydrolase, exo
ECTree
Advanced search results
Engineering
Engineering on EC 3.2.1.94 - glucan 1,6-alpha-isomaltosidase
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D207A
4fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 28fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
D207A/N209A
4.2fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 17.8fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
D266N
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0.011% of wild-type activity, without change in substrate binding ability
M243A
233fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 334fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
D207A
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4fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 28fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
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D207A/N209A
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4.2fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 17.8fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
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M243A
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233fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 334fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
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