3.2.1.84: glucan 1,3-alpha-glucosidase
This is an abbreviated version!
For detailed information about glucan 1,3-alpha-glucosidase, go to the full flat file.
Word Map on EC 3.2.1.84
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3.2.1.84
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oligosaccharide
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n-linked
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trimming
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monoglucosylated
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glucosyltransferase
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calnexin
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glucosidases
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castanospermine
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deglucosylation
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protein-linked
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mannosidase
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man9glcnac2
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udp-glc:glycoprotein
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udp-glucose:glycoprotein
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glc2man9glcnac2
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prkcsh
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alpha-d-glucopyranoside
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high-mannose-type
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deoxynojirimycin
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hepatocystin
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endomannosidase
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glycoprotein-processing
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endoglucosaminidase
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alpha-1,3-linked
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mannose-type
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bromoconduritol
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unglucosylated
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1-deoxymannojirimycin
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ganab
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biotechnology
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medicine
- 3.2.1.84
- oligosaccharide
-
n-linked
-
trimming
-
monoglucosylated
-
glucosyltransferase
- calnexin
-
glucosidases
- castanospermine
-
deglucosylation
-
protein-linked
-
mannosidase
- man9glcnac2
-
udp-glc:glycoprotein
-
udp-glucose:glycoprotein
- glc2man9glcnac2
- prkcsh
- alpha-d-glucopyranoside
-
high-mannose-type
- deoxynojirimycin
-
hepatocystin
- endomannosidase
-
glycoprotein-processing
-
endoglucosaminidase
-
alpha-1,3-linked
-
mannose-type
- bromoconduritol
-
unglucosylated
- 1-deoxymannojirimycin
- ganab
- biotechnology
- medicine
Reaction
Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans =
Synonyms
alpha-(1->3)-glucanase, exo-1,3-alpha-glucanase, exomutanase, glucosidase II, MalA, mutanase, Mutanase RM1, Saci1160
ECTree
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Specific Activity
Specific Activity on EC 3.2.1.84 - glucan 1,3-alpha-glucosidase
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0.178
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after incubation for 1 h at 45°C, medium supplementation with 2.0% cell wall preparation of Laetiporus sulphureus
1.72
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after incubation for 1 h at 45°C, medium supplementation with 0.4% cell wall preparation of Laetiporus sulphureus
115.6
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purified enzyme with lyophilized Laetiporus sulphureus cell walls, pH 5.5, 45°C
5.6
E16590
incubation at 35°C for 10 min, stop reaction by adding 0.4 ml of dinitrosalicylic acid solution, N-terminal domain with mutan-binding activity, C-terminal domain with mutanase activity and only low mutan-binding activity
additional information
E16590
the specific activity for degradation of Streptococcus mutans biofilms is 1.4 micromol/ml after incubation at 35°C at pH 6.0 for 3 min, degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats
additional information
-
the specific activity for degradation of Streptococcus mutans biofilms is 1.4 micromol/ml after incubation at 35°C at pH 6.0 for 3 min, degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats
additional information
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medium supplementation with more than 0.4% cell wall preparation results in lower specific activity