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3.2.1.84: glucan 1,3-alpha-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,3-alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.84

Reaction

Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans =

Synonyms

alpha-(1->3)-glucanase, exo-1,3-alpha-glucanase, exomutanase, glucosidase II, MalA, mutanase, Mutanase RM1, Saci1160

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.84 glucan 1,3-alpha-glucosidase

Purification

Purification on EC 3.2.1.84 - glucan 1,3-alpha-glucosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2-fold purification and 98% residual enzyme activity after precipitation with propanol, around 2-fold purification and 77% enzyme recovery after salting out with ammonium sulfate at 50% saturation, 10-fold concentrated preparation of the enzyme with a yield of 98% after ultrafiltration, mutanase recovery of 97% after lyophilization and concentration of the culture broth in a vacuum evaporator
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by chromatographic procedures
E16590
native extracellular enzyme 18.4fold by ultrafiltration, anion exchange and hydrophobic interaction chromatography, and chromatofocusing to homogeneity
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yield of 56.3% of alkali-soluble and water-insoluble D-glucan
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