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3.2.1.82: exo-poly-alpha-digalacturonosidase

This is an abbreviated version!
For detailed information about exo-poly-alpha-digalacturonosidase, go to the full flat file.

Word Map on EC 3.2.1.82

Reaction

[(1->4)-alpha-D-galacturonosyl]n
+
H2O
=
alpha-D-galacturonosyl-(1->4)-D-galacturonate
+
[(1->4)-alpha-D-galacturonosyl]n-2

Synonyms

Exo-PG, exo-PHase, exo-polygalacturonase, exopolygalacturanosidase, exopolygalacturonase, exopolygalacturonosidase, PGase I, PGI, PGII, polygalacturonase, polygalacturonate hydrolase, YeGH2

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.82 exo-poly-alpha-digalacturonosidase

Purification

Purification on EC 3.2.1.82 - exo-poly-alpha-digalacturonosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 200fold by ammonium sulfate fractionation, dialysis, and gel filtration
-
PGI and PGII purified by gel filtration, PGI 12fold purified to homogeneity
-