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3.2.1.74: glucan 1,4-beta-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,4-beta-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.74

Reaction

(Glcbeta(1-4))r
+
H2O
=
(Glcbeta(1-4))r-1
 +
beta-D-glucopyranose

Synonyms

1,4-beta-D-glucan glucanohydrolase, 1,4-beta-D-glucan glucohydrolase, b-glucan glucohydrolase, beta-(1-4)-glucanase, beta-1,4-beta-glucanase, beta-1,4-glucosidase, beta-glucosidase, beta-glucosidase/xylosidase, Bgl, BglA, Carboxymethyl cellulase, CbhA, Cel48A, Cel9A, Cel9D, cellobiase, cellobiohydrolase, Cellulase, Cex, endo-1,4-beta-D-glucanase, endo/exocellulase, Ex-1, exo-1,4-beta-glucanase, exo-1,4-beta-glucosidase, exo-beta-1,4-glucanase, exo-beta-1,4-glucosidase, exo-bets-1,4-glucanase, exocellulase, exocellulase I, exoglucanase, family 9 glycoside hydrolase, family-1 glycosyl hydrolase, FiG, GH9, glucan 1,4-beta-glucosidase, glucohydrolase, NA23_08975, P-42, Rubg3A, Rubg3B, Rucel5B, TSC, Xyn10E

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.74 glucan 1,4-beta-glucosidase

Specific Activity

Specific Activity on EC 3.2.1.74 - glucan 1,4-beta-glucosidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0011
-
P-42, cotton, pH 5, at 30ºC
0.0012
-
P-42, avicel, pH 5, at 30ºC
0.0021
-
P-42, bacterial cellulose, pH 5, at 30ºC
0.0142
-
Ex-1, cotton, pH 5, at 30ºC
0.0145
-
Ex-1, bacterial cellulose, pH 5, at 30ºC
0.0209
-
Ex-1, avicel, pH 5, at 30ºC
0.0243
-
Ex-1, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30ºC
0.0244
-
P-42, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30ºC
0.025
-
cellulose MN300
0.026
-
Ex-1, carboxymethyl cellulose, pH 5, at 30ºC
0.03
-
cellulose HBS
0.0311
-
P-42, carboxymethyl cellulose, pH 5, at 30ºC
0.04
-
using filter paper as substrate, at 60°C in pH 6.5 citrate buffer
0.067
-
crude enzyme from homogenate, using cellobiose as substrate, pH and temperature not specified in the publication
0.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
0.102
-
H3PO4 swollen avicel
0.108
-
HCl swollen avicel
0.128
-
celloheptaose
0.228
-
after 3.4fold purification, using cellobiose as substrate, pH and temperature not specified in the publication
0.236
-
cellohexaose
0.323
-
pH 6.5, at 37ºC
0.35
2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 40°C
0.42
-
cellopentaose
0.65
2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 42°C
0.684
-
cellotetraose
0.7
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
1.14
-
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 5.0, 50°C
10.7
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
10.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
11.5
4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 42°C
12.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
12.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
129
p-nitrophenyl-beta-D-fucopyranoside as substrate
13.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
133
p-nitrophenyl-beta-D-galactopyranoside as substrate
14.5
-
bacterial cellulose powder, not dried, isoenzyme II
14.6
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
15.1
-
bacterial cellulose powder, not dried, isoenzyme I
177
p-nitrophenyl-beta-D-glucopyranoside as substrate
2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
2.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
2.53
-
cellulose acetate, pH 5.0
2.57
-
substrate carboxymethyl cellulose, pH 5.0, 50°C
2.6
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
20.9
-
avicel, isoenzyme II
22.6
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
220
-
using carboxymethylcellulose as substrate, at 65°C in pH 5.5 citrate buffer
23.3
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
23.5
-
using phosphate acid swollen cellulose as substrate, at 60°C in pH 6.5 citrate buffer
25.5
-
carboxymethyl cellulose, isoenzyme I
27.1
-
p-nitrophenyl beta-D-lactoside, isoenzyme II
27.3
-
p-nitrophenyl beta-D-lactoside, isoenzyme I
3.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
3.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
3.3
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
3.36
-
cellulose, pH 5.0
3.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
3.583
-
alpha-cellulose, pH 5.0
3.7
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
3.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
33.2
-
p-nitrophenyl beta-D-celloside, isoenzyme II
33.5
cellobiose as substrate, pH 4.6, 40°C
34
-
p-nitrophenyl beta-D-celloside, isoenzyme I
4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
41
4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 40°C
5.3
cellobiose as substrate, pH 4.6, 42°C
52.9
-
using 4-nitrophenyl beta-D-cellobioside as substrate, at 65°C in pH 5.5 citrate buffer
58.79
Thermomonospora sp.
-
carboxymethylcellulase activity
6.8
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
693
-
using lichenan as substrate, at 60°C in pH 6.5 citrate buffer
7.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
717
-
using barley glucan as substrate, at 60°C in pH 6.5 citrate buffer
77
4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
8.09
-
substrate 4-nitrophenyl beta-D-cellobioside, pH 5.0, 50°C
9.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
9.5
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
additional information