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3.2.1.74: glucan 1,4-beta-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,4-beta-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.74

Reaction

(Glcbeta(1-4))r
+
H2O
=
(Glcbeta(1-4))r-1
 +
beta-D-glucopyranose

Synonyms

1,4-beta-D-glucan glucanohydrolase, 1,4-beta-D-glucan glucohydrolase, b-glucan glucohydrolase, beta-(1-4)-glucanase, beta-1,4-beta-glucanase, beta-1,4-glucosidase, beta-glucosidase, beta-glucosidase/xylosidase, Bgl, BglA, Carboxymethyl cellulase, CbhA, Cel48A, Cel9A, Cel9D, cellobiase, cellobiohydrolase, Cellulase, Cex, endo-1,4-beta-D-glucanase, endo/exocellulase, Ex-1, exo-1,4-beta-glucanase, exo-1,4-beta-glucosidase, exo-beta-1,4-glucanase, exo-beta-1,4-glucosidase, exo-bets-1,4-glucanase, exocellulase, exocellulase I, exoglucanase, family 9 glycoside hydrolase, family-1 glycosyl hydrolase, FiG, GH9, glucan 1,4-beta-glucosidase, glucohydrolase, NA23_08975, P-42, Rubg3A, Rubg3B, Rucel5B, TSC, Xyn10E

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.74 glucan 1,4-beta-glucosidase

Purification

Purification on EC 3.2.1.74 - glucan 1,4-beta-glucosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
15fold
Sporotrichum pulverulentum
-
ammonium sulfate precipitation, Macro-Prep DEAE column chromatography, methyl hydrophobic-interaction column chromatography, and Superdex 200 gel filtration
-
by nickel affinity chromatography and gel filtration chromatography
-
catalytic and cellulose binding domains by cellulose FPLC column chromatography, ultrafiltration and papain treatment
-
centrifugation, streptomycin sulfate precipitation, ammonium sulfate fractionation, DEAE column chromatography, IMAC-Zn2+ chromatography
-
cloned Cel48A, centrifugation, phenylmethanesulfonyl fluoride and ammonium sulfate fractionation, phenyl-Sepharose column, Q-Sepharose column
gel filtration
-
native Cel48A, HPLC
Ni-nitrilotriacetatic acid agarose column chromatography
Ni-NTA column chromatography
-
Ni-NTA resincolumn chromatography
-
Ni2+ affinity column chromatography
precipitation with acetone, gel filtration chromatography on Sephadex G-100 and DEAE-cellulose chromatography, 47.14fold
-
Profinia affinity chromatography protein purification system
-
Superdex 200 gel filtration
-
TSC by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration
Thermomonospora sp.
-
wild-type and mutants purified by immobilized metal affinity chromatography. Wild-type further purified by gel filtration