3.2.1.74: glucan 1,4-beta-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,4-beta-glucosidase, go to the full flat file.

Reaction

Synonyms

1,4-beta-D-glucan glucanohydrolase, 1,4-beta-D-glucan glucohydrolase, b-glucan glucohydrolase, beta-(1-4)-glucanase, beta-1,4-beta-glucanase, beta-1,4-glucosidase, beta-glucosidase, beta-glucosidase/xylosidase, Bgl, BglA, Carboxymethyl cellulase, CbhA, Cel48A, Cel9A, Cel9D, cellobiase, cellobiohydrolase, Cellulase, Cex, endo-1,4-beta-D-glucanase, endo/exocellulase, Ex-1, exo-1,4-beta-glucanase, exo-1,4-beta-glucosidase, exo-beta-1,4-glucanase, exo-beta-1,4-glucosidase, exo-bets-1,4-glucanase, exocellulase, exocellulase I, exoglucanase, family 9 glycoside hydrolase, family-1 glycosyl hydrolase, FiG, GH9, glucan 1,4-beta-glucosidase, glucohydrolase, NA23_08975, P-42, Rubg3A, Rubg3B, Rucel5B, TSC, Xyn10E

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.74 glucan 1,4-beta-glucosidase

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Results	in table
7	Activating Compound
66	AA Sequence
7	Application
1	CAS Registry Number
18	Cloned(Commentary)
4	Crystallization (Commentary)
65	Inhibitors
3	kcat/KM [mM/s]
13	Ki Value [mM]
53	KM Value [mM]
45	Localization
38	Metals/Ions
37	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
4	Organic Solvent Stability
119	Organism
4	Pathway
3	PDB ID
52	pH Optimum
4	pH Range
13	pH Stability
4	pI Value
11	Posttranslational Modification
33	Protein Variants
34	Purification (Commentary)
1	Reaction
6	Reaction Type
106	Reference
1	Renatured (Commentary)
22	Source Tissue
179	Specific Activity [micromol/min/mg]
1	Storage Stability
315	Substrates and Products (Substrate)
36	Subunits
107	Synonyms
1	Systematic Name
52	Temperature Optimum [°C]
6	Temperature Range [°C]
26	Temperature Stability [°C]
13	Turnover Number [1/s]

Metals Ions

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Metals Ions on EC 3.2.1.74 - glucan 1,4-beta-glucosidase

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Ba2+

Fervidobacterium islandicum

-

122% activity at 5 mM

751309

Ca2+

Fervidobacterium islandicum

-

105.7% activity at 5 mM

751309

CaCl2

2 entries

Co2+

2 entries

CoCl2

2 entries

CuCl2

2 entries

D-fructose

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 102.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C

717112

D-glucose

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM,82% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C

717112

D-xylose

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 106.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C

717112

Fe3+

3 entries

fructose

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 107.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C

717112

glucose

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM,24.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C

717112

MgCl2

2 entries

Mn2+

4 entries

MnCl2

3 entries

Ni2+

Fervidobacterium islandicum

-

116.2% activity at 5 mM

751309

NiCl2

2 entries

xylose

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 88% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C

717112

ZnSO4

2 entries

additional information

5 entries

CaCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 107.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C

717112

CaCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 92.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C

717112

Co2+

Chaetomium globosum

-

5 min at 50ºC, 1 mM, 103.0% of the relative activity without metal ions

654146

Co2+

Chaetomium globosum

-

5 min at 50ºC, 10 mM, 101.9% of the relative activity without metal ions

654146

CoCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 79.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C

717112

CoCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 85.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C

717112

CuCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 79.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C

717112

CuCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 83.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C

717112

Fe3+

Chaetomium globosum

-

5 min at 50ºC, 1 mM, 105.5% of the relative activity without metal ions

654146

Fe3+

Chaetomium globosum

-

5 min at 50ºC, 10 mM, 103.6% of the relative activity without metal ions

654146

Fe3+

Trichoderma virens

A0A288W730

about 150% at 2.5 mM

749546

MgCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 110.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C

717112

MgCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 96.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C

717112

Mn2+

Chaetomium globosum

-

5 min at 50ºC, 1 mM, 105.8% of the relative activity without metal ions

654146

Mn2+

Chaetomium globosum

-

5 min at 50ºC, 10 mM, 104.1% of the relative activity without metal ions

654146

Mn2+

Fervidobacterium islandicum

-

108.3% activity at 5 mM

751309

Mn2+

Trichoderma virens

A0A288W730

about 160% at 2.5 mM

749546

MnCl2

Ruminiclostridium papyrosolvens

-

activity increased by 58%

136842

MnCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 102.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C

717112

MnCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 112% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C

717112

NiCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 76.7% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C

717112

NiCl2

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 86.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C

717112

ZnSO4

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 111.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C

717112

ZnSO4

uncultured rumen bacterium

G3BJX3, G3BJX4

5 mM, 90.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C

717112

additional information

Fervidobacterium islandicum

-

not affected by Mg2+

751309

additional information

Stereum hirsutum

-

divalent cations are not required for enzyme activation

713875

additional information

Trichoderma virens

A0A288W730

not influenced by Zn2+, Ca2+, Mg2+ and Cu2+

749546

additional information

uncultured bacterium

-

the addition of 5 mM Zn2+, Co2+ and Cu2+ has no statistical difference on enzyme activity

715960

additional information

Vibrio cholerae serotype O1

-

NaCl and KCl gives optimum results at 10 mM, at 37ºC, pH 6.5

656066