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3.2.1.65: levanase

This is an abbreviated version!
For detailed information about levanase, go to the full flat file.

Word Map on EC 3.2.1.65

Reaction

Glcpalpha(1-6)[Frufbeta(2-6)]n
+
H2O
=
Glcpalpha(1-6)[Frufbeta(2-6)]n-m
+
[Frufbeta(2-6)]m

Synonyms

2,6-beta-D fructan fructohydrolase, 2,6-beta-D-fructan fructanohydrolase, 6-FEHs, endo-levanase, endolevanase, exo-levanase, exolevanase, fructan-6-exohydrolase, fructose-releasing exo-levanase, LeA, levan hydrolase, levanase, levansucrase, LevB1, LevP, LsdB, ORF477, SacB

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.65 levanase

Purification

Purification on EC 3.2.1.65 - levanase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and Sephadex A-50 gel filtration
-
by immobilization-nickel affinity chromatography, centrifugation, column chromatography and dialysis
-
expression in Escherichia coli
-
HisTrap column chromatography, and Superdex 200 gel filtration
HiTrap column chromatography
-
ion exchange column chromatography
-
native extracellular enzyme 397fold from culture supernatant by ammonium sulfate fractionation, dialysis, gel filtration, and anion exchange chromatography
-
phenyl Sepharose column chromatography and Q Sepharose chromatography
-