3.2.1.63: 1,2-alpha-L-fucosidase
This is an abbreviated version!
For detailed information about 1,2-alpha-L-fucosidase, go to the full flat file.
Word Map on EC 3.2.1.63
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3.2.1.63
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beta-galactosidase
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alpha-mannosidase
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beta-glucuronidase
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beta-glucosidase
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alpha-galactosidase
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alpha-glucosidase
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glycosidases
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n-acetyl-beta-glucosaminidase
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fucosylated
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beta-hexosaminidase
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beta-n-acetylglucosaminidase
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beta-mannosidase
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fucosidosis
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beta-xylosidase
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3.2.1.51
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2'-fucosyllactose
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arylamidase
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corporis
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i-cell
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lacto-n-fucopentaose
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mucolipidosis
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medicine
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biotechnology
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diagnostics
- 3.2.1.63
- beta-galactosidase
- alpha-mannosidase
- beta-glucuronidase
- beta-glucosidase
- alpha-galactosidase
- alpha-glucosidase
- glycosidases
- n-acetyl-beta-glucosaminidase
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fucosylated
- beta-hexosaminidase
- beta-n-acetylglucosaminidase
- beta-mannosidase
- fucosidosis
- beta-xylosidase
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3.2.1.51
- 2'-fucosyllactose
- arylamidase
- corporis
- i-cell
- lacto-n-fucopentaose
- mucolipidosis
- medicine
- biotechnology
- diagnostics
Reaction
Synonyms
1,2-alpha-L-fucosidase, Afc3, AfcA, AfcA protein, alpha(1,2) fucosidase, alpha-(1--> 2)-L-fucosidase, alpha-fucosidase, alpha-L-fucosidase, alpha-L-fucosidase II, alpha1,2-FUC, alpha1,2-fucosidase, bfo_2737, FgFCO1, Fuc1, FucNaFLD, fucosidase, 1,2-alpha-L-, More
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Substrates Products
Substrates Products on EC 3.2.1.63 - 1,2-alpha-L-fucosidase
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REACTION DIAGRAM
2'-fucosyllactose + H2O
?
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hydrolysis by the Fuc domain of AfcA. N423 and D766 are critical for activating an attacking water molecule and N421 is critical for maintaining the water molecule and the side-chain of E566 (a general acid) in their proper orientations
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-
?
4-nitrophenyl-alpha-L-fucoside + H2O
4-nitrophenol + L-fucose
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-
-
?
alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glc + H2O
alpha-L-fucose + beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glc
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i.e. lacto-N-fucopentaose I
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?
alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4-)-beta-Glc + H2O
alpha-L-fucose + beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glc
Bacillus fulminans
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-
-
-
?
alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + H2O
L-fucose + ?
i.e. blood group antigen H pentaose type-1, weak substrate
-
-
?
alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-D-Gal + H2O
L-fucose + ?
i.e. blood group antigen H-tetraose type 2
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-
?
alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-D-GlcNAc + H2O
L-fucose + ?
i.e. blood group antigen H triose type-2
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-
?
beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-beta-D-GalNAc-(1->3)-D-Gal
L-fucose + ?
i.e. Lewis a tetraose
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-
?
beta-L-fucosyl fluoride + lactose + H2O
?
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Hehre resynthesis-hydrolysis is catalyzed by the wild-type Fuc domain of AfcA. Conversion of the inverting alpha-glycosidase to a glycosynthase
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-
?
Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc + H2O
L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
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i.e. lacto-N-fucopentaose I, at 29% of the activity with 2'-fucosyllactose
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-
?
Fucalpha(1-2)Galbeta(1-4)Glc + H2O
L-fucose + Galbeta(1-4)Glc
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i.e. 2'-fucosyllactose
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-
?
Galbeta(1-4)(Fucalpha(1-3))Glc + H2O
?
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i.e. 3-fucosyllactose, at 0.85% of the activity with 2'-fucosyllactose
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-
?
methyl-2-alpha-L-fucopyranosyl-beta-D-galactoside + H2O
L-fucose + methyl beta-D-galactoside
N-acetyl-propargyl alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc + H2O
L-fucose + ?
i.e. blood group antigen H-pentaose type-2 beta-N-acetyl propargyl
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-
?
porcine gastric mucin + H2O
?
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at 33% of the activity with 2'-fucosyllactose
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-
?
submaxillary glycoprotein + H2O
L-fucose + ?
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degradation of blood group substances
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?
2'-fucosyllactose + H2O
L-fucose + lactose
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simulation of the reaction by the combined quantum mechanical and molecular mechanical approach. Molecular dynamics simulations and free energy profiles support that the overall reaction is a stepwise mechanism
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-
?
L-fucose + lactose
reaction with recombinant Fuc domain
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-
?
2'-L-fucosyllactose + H2O
L-fucose + lactose
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reaction with recombinant Fuc domain
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-
?
L-fucose + galactose
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-
-
?
2-O-alpha-L-fucopyranosyl-D-galactose + H2O
L-fucose + galactose
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-
-
?
4-methylumbelliferone + L-fucopyranose
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-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + L-fucopyranose
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-
-
?
4-nitrophenol + L-fucopyranose
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-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucopyranose
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-
-
-
?
L-fucose + ?
i.e. blood group antigen B-tetraose type 2, weak substrate
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-
?
alpha-D-Gal-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->4)-D-GlcNAc + H2O
L-fucose + ?
i.e. blood group antigen B-tetraose type 2, weak substrate
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-
?
?
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the enzyme also possesses alpha(1,6) specificity on small unbranched substrates
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-
?
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O
?
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the enzyme also possesses alpha(1,6) specificity on small unbranched substrates
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?
?
74% of the activity with 2'-fucosyllactose, reaction with recombinant Fuc domain
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?
blood group substance H + H2O
?
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74% of the activity with 2'-fucosyllactose, reaction with recombinant Fuc domain
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-
?
?
61% of the activity with 2'-fucosyllactose, reaction with recombinant Fuc domain
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-
?
lacto-N-fucopentaose I + H2O
?
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61% of the activity with 2'-fucosyllactose, reaction with recombinant Fuc domain
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-
?
L-fucose + methyl beta-D-galactoside
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-
-
?
methyl-2-alpha-L-fucopyranosyl-beta-D-galactoside + H2O
L-fucose + methyl beta-D-galactoside
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-
-
-
?
mucin + H2O
L-fucose + ?
Bacillus fulminans
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porcine submaxillary and glycopeptide of desialyzed mucin
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-
?
L-fucose + lactose
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i.e. 2-O-alpha-L-fucosyllactose
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?
O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O
L-fucose + lactose
Bacillus fulminans
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i.e. 2-O-alpha-L-fucosyllactose
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?
O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O
L-fucose + lactose
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i.e. 2-O-alpha-L-fucosyllactose
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?
?
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highly specific for non-reducing terminal L-fucose residues linked to D-galactose residues by 1,2-alpha linkage
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-
?
additional information
?
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inductively produced in culture medium containing porcine gastric mucin, weakly induced by L-fucose and D-arabinose, but not by other sugars including D-glucose
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?
additional information
?
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the recombinant Fuc domain shows no activity with 3-fucosyllactose, lacto-N-fucopentaose II, lacto-N-fucopentaose V and blood group substance B
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-
?
additional information
?
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the recombinant Fuc domain shows no activity with 3-fucosyllactose, lacto-N-fucopentaose II, lacto-N-fucopentaose V and blood group substance B
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-
?
additional information
?
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the recombinant Fuc domain shows no activity with 3-fucosyllactose, lacto-N-fucopentaose II, lacto-N-fucopentaose V and blood group substance B
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-
?
additional information
?
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the recombinant Fuc domain shows no activity with 3-fucosyllactose, lacto-N-fucopentaose II, lacto-N-fucopentaose V and blood group substance B
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-
?
additional information
?
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not: p-nitrophenyl-alpha-L-fucoside, 2-O-, 3-O- and 4-O-alpha-L-fucopyranosylfucoses, 3-O- and 4-O-alpha-L-fucopyranosyl-D-galactoses, action on oligosaccharides and glycoproteins, not: simple methyl nitrophenyl fucosides
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-
?
additional information
?
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the enzyme does not hydrolyze other fucosyloligosaccharides like Lewisa trisaccharide, Lewisx trisaccharide, 3-fucosyl-GlcNAc, 4-fucosyl-GlcNAc, 6-fucosyl-GlcNAc or 4-nitrophenyl-alpha-L-fucoside
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-
?
additional information
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substrate specificity, overview. No activity with 3-fucosyllactose. The enzyme is also active on xyloglucan
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?
additional information
?
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substrate specificity, overview. No activity with 3-fucosyllactose. The enzyme is also active on xyloglucan
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?
additional information
?
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highly specific for non-reducing terminal L-fucose residues linked to D-galactose residues by 1,2-alpha linkage
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?
additional information
?
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not active with biotin-PAA-alpha-D-galactose
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?
additional information
?
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the alpha-L-fucosidase domain of the protein accepts both alpha1-2 and alpha1-4 linked fucosylated glycans as substrates and has high affinity for a Fuc-alpha-1,2-Gal motif
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-
?
additional information
?
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not active with biotin-PAA-alpha-D-galactose
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?
additional information
?
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the alpha-L-fucosidase domain of the protein accepts both alpha1-2 and alpha1-4 linked fucosylated glycans as substrates and has high affinity for a Fuc-alpha-1,2-Gal motif
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-
?
additional information
?
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the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal
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-
?
additional information
?
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the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal
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?