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3.2.1.6: endo-1,3(4)-beta-glucanase

This is an abbreviated version!
For detailed information about endo-1,3(4)-beta-glucanase, go to the full flat file.

Word Map on EC 3.2.1.6

Reaction

(Glcbeta(1-3))n
+
H2O
=
Laminaripentaose
 +
(Glcbeta(1-3))n-5

Synonyms

1,3-(4)-beta-glucanase (endoglucanase), 1,3-1,4-beta-glucanase EII (barley), 1,3-beta-D-glucanase, AFUA_1G05290, AFUA_2G14360, AFUA_4G13360, AFUA_5G02280, Agl9A, AkLam36, barley-beta-D-glucanase, beta-1,3-1,4 glucanase, beta-1,3-1,4-glucanase, beta-1,3-glucanase, beta-1,3:1,4 glucanase, beta-D-(1-3)-glucanase, beta-glucanase, bglBC1, CBM-CD, CC1G_01665, Cel8A, CelL, chitosanase, CMCase, CtLic26A, enda-beta-(1-3),(1-4)-glucanase, endo beta-1,3-glucanase IV, endo-1,3(4)-beta-D-glucanase, endo-1,3(4)-beta-glucanase, endo-1,3-1,4-beta-D-glucanase, endo-1,3-beta-D-glucanase, endo-1,3-beta-glucanase, endo-1,4-beta-glucanase, endo-beta-(1-3)-D-glucanase, endo-beta-1,3(4)-glucanase, endo-beta-1,3-1,4-D-glucanase, endo-beta-1,3-1,4-glucanase, endo-beta-1,3-glucanase, endo-beta-1,4-glucanase, eng1, ENG16A, Eng2, Eng3, Eng4, Eng5, Engl1, Fsbeta-glucanase, GluA, H(A16-M), HdLam33, LAM, Lam16A, LamA, lamarinase, laminaranase, laminarinase, laminarinase 16A, laminarinase II, laminarinase Lam16A, LamR, LIC 1, Lic16A, Lic8H, Lichenase, More, nonlytic endo-beta-1,3-glucanase I, SsGlc, TcGlu2, TeEgl5A, Xyn10B

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.6 endo-1,3(4)-beta-glucanase

Cloned

Cloned on EC 3.2.1.6 - endo-1,3(4)-beta-glucanase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain Bl21
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant heterologous enzyme expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3)
-
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
-
expression in Escherichia coli, Enterococcus faecalis, Lactococcus lactis
-
expression in Pichia pastoris
expression in Pichia pastoris. The beta-1,3-1,4-glucanase gene is modified and expressed according to its preferred codon usage
expression in Talaromyces verruculosus
-
expression of five truncated proteins of LamA in Escherichia coli BL21(DE3)
expression of recombinant truncated proteins in Escherichia coli
-
gene bglc8H, DNA and amino acid sequence determination and analysis, phylogenetic tree, cloning in Escherichia coli strain DH5alpha
gene gls, expression of a fragment of gene gls fused to GFP and the acmA gene from Lactococcus lactis, encoding an N-acetylmuraminidase, in Lactococcus lactis strain AS1.2829, the recombinant chimera is functional
-
gene SU40-glu, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression in Escherichia coli strain BL21(DE3)
gene Teegl5A, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain Trans-T1, functional expression in Pichia pastoris strain GS115 and secretion to the culture medium
HdLam33, DNA and amino acid sequence determination and analysis
-
placing of gene eng2+ under the control of a repressible promoter
-
recombinantly expressed in Pichia pastoris
-
subcloning in Escherichia coli strain Dh5alpha, recombinant expression of beta-1,3-1,4-glucanase from Bacillus subtilis fused to alpha-agglutinin and expressed under the control of the GAL1 promoter in Saccharomyces cerevisiae, plasmid construction, overview
-
the enda-beta-(1-3),(1-4)-glucanase gene bglBC1 is modified by substituting its native promoter with a strong promoter, BJ27X, to increase expression of the gene when cloned into Bacillus subtilis RM125 and Bacillus megaterium ATCC14945
-