3.2.1.59: glucan endo-1,3-alpha-glucosidase

This is an abbreviated version!
For detailed information about glucan endo-1,3-alpha-glucosidase, go to the full flat file.

Reaction

Synonyms

Agl-FH1, Agl-FH2, Agl-KA, AglST2, Agn1p, Agn2, Agn2p, alpha-1,3 endoglucanase, alpha-1,3-glucanase, alpha-1,3-glucanase HF65, alpha-1,3-glucanase HF90, cariogenanase, cariogenase, endo alpha-1,3-glucanase, endo-(1,3)-alpha-glucanase, endo-(1-->3)-alpha-glucanase, endo-(1-3)-alpha-glucanase, endo-1,3-alpha-D-glucanase, endo-1,3-alpha-glucanase, endo-alpha-1,3-glucanase, FH11, glucanase, endo-1,3-alpha-mutanase, MuB, MuC1, MuC2, mutanase, MutAp

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.59 glucan endo-1,3-alpha-glucosidase

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Results	in table
4	Activating Compound
22	AA Sequence
15	Application
1	CAS Registry Number
18	Cloned(Commentary)
4	Expression
14	General Information
4	General Stability
43	Inhibitors
6	KM Value [mM]
12	Localization
13	Metals/Ions
15	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
59	Organism
22	pH Optimum
8	pH Range
5	pH Stability
1	pI Value
3	Posttranslational Modification
3	Protein Variants
17	Purification (Commentary)
1	Reaction
1	Reaction Type
52	Reference
16	Source Tissue
6	Specific Activity [micromol/min/mg]
3	Storage Stability
68	Substrates and Products (Substrate)
20	Subunits
71	Synonyms
1	Systematic Name
18	Temperature Optimum [°C]
4	Temperature Range [°C]
16	Temperature Stability [°C]
6	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 3.2.1.59 - glucan endo-1,3-alpha-glucosidase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

21

Trichoderma harzianum

-

diluted enzyme, 1:19, 0.2 M acetate bufferm pH 5.5, at room temperature is stable for 24-48 h

649093

35

Trichoderma harzianum

-

1 h, stable up to

649093, 653287

37

Pseudomonas sp.

-

pH 5.5, 3 days, 3% loss of activity

136688

40

Niallia circulans

-

5% loss of activity after 90 min

136691

45

2 entries

50

3 entries

60

3 entries

65

2 entries

65 - 70

Streptomyces thermodiastaticus

-

the purified enzyme retains more than 50% of maximum activity after incubation at 65°C more than 2 h and exhibits about 95% of full activity after incubation at 65°C for 10 min. After treatment at 70°C for 120 min, the enzyme shows 20% residual activity

751239

45

Niallia circulans

-

25% loss of activity after 90 min

136691

45

Trichoderma harzianum

-

1 h, enzyme loses 23% of its maximal activity

649093

50

Niallia circulans

-

complete loss of activity after 20 min

136691

50

Streptomyces sp.

-

stable up to 10 min

136693

50

Trichoderma viride

-

rapid inactivation

136695

60

Flavobacterium sp.

-

complete loss of activity after 30 min

136694

60

Pseudomonas sp.

-

loss of activity at or above

136688

60

Trichoderma harzianum

-

1 h, complete loss of activity

649093

65

Streptomyces chartreusis

-

pH 6.0, 10 min, complete inactivation

136690

65

Streptomyces thermodiastaticus

-

the residual activity of the enzyme is more than 80% after incubation at 65°C for 1 h

751242