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3.2.1.59: glucan endo-1,3-alpha-glucosidase

This is an abbreviated version!
For detailed information about glucan endo-1,3-alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.59

Reaction

Nigeropentaose
+ 3 H2O = 2 nigerose +
alpha-D-glucopyranose

Synonyms

Agl-FH1, Agl-FH2, Agl-KA, AglST2, Agn1p, Agn2, Agn2p, alpha-1,3 endoglucanase, alpha-1,3-glucanase, alpha-1,3-glucanase HF65, alpha-1,3-glucanase HF90, cariogenanase, cariogenase, endo alpha-1,3-glucanase, endo-(1,3)-alpha-glucanase, endo-(1-->3)-alpha-glucanase, endo-(1-3)-alpha-glucanase, endo-1,3-alpha-D-glucanase, endo-1,3-alpha-glucanase, endo-alpha-1,3-glucanase, FH11, glucanase, endo-1,3-alpha-mutanase, MuB, MuC1, MuC2, mutanase, MutAp

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.59 glucan endo-1,3-alpha-glucosidase

Purification

Purification on EC 3.2.1.59 - glucan endo-1,3-alpha-glucosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Agn2p purified by immobilized metal ion affinity chromatography followed by anion exchange chromatography
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ammonium sulfate precipitation, DEAE-cellulofine column chromatography and butyl-Toyopearl 650M column chromatography
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DEAE-cellufine A500 column chromatography and HiTrap Q column chromatography
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NRRL-B-12324
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recombinant secreted wild-type and deletion mutant enzymes from Escherichia coli strain Rosetta-gami B (DE3) culture supernatant by dialysis, anion exchange chromatography, ammonium sulfate fractionation and hydrophobic interaction chromatography
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