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3.2.1.59: glucan endo-1,3-alpha-glucosidase

This is an abbreviated version!
For detailed information about glucan endo-1,3-alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.59

Reaction

Nigeropentaose
+ 3 H2O = 2 nigerose +
alpha-D-glucopyranose

Synonyms

Agl-FH1, Agl-FH2, Agl-KA, AglST2, Agn1p, Agn2, Agn2p, alpha-1,3 endoglucanase, alpha-1,3-glucanase, alpha-1,3-glucanase HF65, alpha-1,3-glucanase HF90, cariogenanase, cariogenase, endo alpha-1,3-glucanase, endo-(1,3)-alpha-glucanase, endo-(1-->3)-alpha-glucanase, endo-(1-3)-alpha-glucanase, endo-1,3-alpha-D-glucanase, endo-1,3-alpha-glucanase, endo-alpha-1,3-glucanase, FH11, glucanase, endo-1,3-alpha-mutanase, MuB, MuC1, MuC2, mutanase, MutAp

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.59 glucan endo-1,3-alpha-glucosidase

Cloned

Cloned on EC 3.2.1.59 - glucan endo-1,3-alpha-glucosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Agn1p signal peptide (amino acids 1-20) cloned between the XhoI and NheI sites of pND092, producing pND054
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expressed in Aspergillus oryzae
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expressed in Bacillus subtilis ISW 1214 cells
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expressed in Escherichia coli BL21 (DE3) cells
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli Rosetta-gami B (DE3) cells
expressed in Escherichia coli Turner (DE3) cells
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expression in Aspergillus oryzae
gene agn1, sequence comparisons and phylogenetic analysis, heterologous expression in Schizosaccharomyces pombe agn1 null mutant strain 1252. Complementation of a Schizosaccharomyces pombe agn1DELTA strain with the Paracoccidioides brasiliensis AGN1 gene restores the wild-type phenotype, indicating functionality of the gene
gene Pc12g07500, heterologous expression in Saccharomyces cerevisiae strain IME208 confirms that Pc12g07500 encodes an active alpha-1,3 endoglucanase, secretion of the recombinant enzyme to culture medium
gene Pc12g13330, heterologous expression in Saccharomyces cerevisiae strain IME208 confirmes that Pc12g13330 encodes an inactive alpha-1,3 endoglucanase, secretion of the recombinant enzyme to culture medium
recombinant expression of wild-type and deletion mutant enzymes in Escherichia coli strain Rosetta-gami B (DE3), secretion of enzyme to the culture medium, recombinant expression as GFP-tagged proteins, overview
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tagged Agn2p at its carboxyl terminus with six histidines, expression in Escherichia coli
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