3.2.1.59: glucan endo-1,3-alpha-glucosidase
This is an abbreviated version!
For detailed information about glucan endo-1,3-alpha-glucosidase, go to the full flat file.
Word Map on EC 3.2.1.59
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3.2.1.59
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mutans
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glucans
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dental
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trichoderma
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dextranase
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harzianum
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paenibacillus
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circulans
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caries
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streptococcal
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medicine
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water-insoluble
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protoplast-forming
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cariogenic
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schizophyllum
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saccharification
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glucanohydrolases
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endolysis
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laetiporus
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sobrinus
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endo-beta-1,3-glucanase
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curdlanolyticus
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sulphureus
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rosetta-gami
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pharmacology
- 3.2.1.59
- mutans
- glucans
-
dental
- trichoderma
- dextranase
- harzianum
- paenibacillus
- circulans
- caries
- streptococcal
- medicine
-
water-insoluble
-
protoplast-forming
-
cariogenic
-
schizophyllum
-
saccharification
-
glucanohydrolases
-
endolysis
-
laetiporus
- sobrinus
- endo-beta-1,3-glucanase
- curdlanolyticus
- sulphureus
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rosetta-gami
- pharmacology
Reaction
Synonyms
Agl-FH1, Agl-FH2, Agl-KA, AglST2, Agn1p, Agn2, Agn2p, alpha-1,3 endoglucanase, alpha-1,3-glucanase, alpha-1,3-glucanase HF65, alpha-1,3-glucanase HF90, cariogenanase, cariogenase, endo alpha-1,3-glucanase, endo-(1,3)-alpha-glucanase, endo-(1-->3)-alpha-glucanase, endo-(1-3)-alpha-glucanase, endo-1,3-alpha-D-glucanase, endo-1,3-alpha-glucanase, endo-alpha-1,3-glucanase, FH11, glucanase, endo-1,3-alpha-mutanase, MuB, MuC1, MuC2, mutanase, MutAp
ECTree
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Cloned
Cloned on EC 3.2.1.59 - glucan endo-1,3-alpha-glucosidase
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Agn1p signal peptide (amino acids 1-20) cloned between the XhoI and NheI sites of pND092, producing pND054
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expressed in Escherichia coli Rosetta-gami B (DE3) cells
expression in Aspergillus oryzae
gene agn1, sequence comparisons and phylogenetic analysis, heterologous expression in Schizosaccharomyces pombe agn1 null mutant strain 1252. Complementation of a Schizosaccharomyces pombe agn1DELTA strain with the Paracoccidioides brasiliensis AGN1 gene restores the wild-type phenotype, indicating functionality of the gene
gene Pc12g07500, heterologous expression in Saccharomyces cerevisiae strain IME208 confirms that Pc12g07500 encodes an active alpha-1,3 endoglucanase, secretion of the recombinant enzyme to culture medium
gene Pc12g13330, heterologous expression in Saccharomyces cerevisiae strain IME208 confirmes that Pc12g13330 encodes an inactive alpha-1,3 endoglucanase, secretion of the recombinant enzyme to culture medium
recombinant expression of wild-type and deletion mutant enzymes in Escherichia coli strain Rosetta-gami B (DE3), secretion of enzyme to the culture medium, recombinant expression as GFP-tagged proteins, overview
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tagged Agn2p at its carboxyl terminus with six histidines, expression in Escherichia coli
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