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3.2.1.54: cyclomaltodextrinase

This is an abbreviated version!
For detailed information about cyclomaltodextrinase, go to the full flat file.

Word Map on EC 3.2.1.54

Reaction

Cyclomaltodextrin
+
H2O
=
linear maltodextrin

Synonyms

AfCda13, AglB, alpha-amylase, alpha-amylase II, CD-/pullulan-hydrolyzing enzyme, CD-ase, CD-degrading enzyme, CD-hydrolyzing amylase, CDA, CDase, CDase I-5, CMD, cyclodextrinase, cycloheptaglucanase, cyclohexaglucanase, cyclomaltodextrin dextrin-hydrolase, Cyclomaltodextrin hydrolase, decycling, cyclomaltodextrinase, CymH, cytoplasmic decycling maltodextrinase, EC 3.2.1.12, Env cda13A, FspCMD, H-17 CDase, H-17 thermostable CDase, LLCD, LsCda13, Lsp26X-Mdase, maltodextrin glucosidase, More, neopullulanase, PFTA, RA.04, thermophilic CDase, TK1770, TVA II

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.54 cyclomaltodextrinase

Purification

Purification on EC 3.2.1.54 - cyclomaltodextrinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by DEAE-Toyopearl chromatography and ultrafiltration
-
by Ni-chelating column HisTrap HP chromatography
-
by Q-sepharose column, DEAE-Sepharose column, Bioprep SE 1000/17 and UNO S1 column chromatography
-
gene encoding enzyme cloned in Escherichia coli, expressed and purified 12fold from Escherichia coli
-
gene encoding enzyme cloned in Escherichia coli, expressed and purified from Escherichia coli
-
HisTrap nickel column chromatography
ion-exchange chromatography
Ni-chelating chromatography
Ni-NTA column chromatography, and Superdex 200 gel filtration
-
not completely purified, minor contamination in ultracentrifugation
-
of the recombinant protein
of the recombinant protein by filtration of the supernatant
-
partially purified 205fold
-
purified 252fold
-
purified 40fold, highly purified, not completely free from contaminations
-
purified to 93% homogeneity
-
purified to 94% homogeneity
-
purified to homogeneity
-
recombinant
-
recombinant enzyme from Escherichia coli by ammonium sulfate precipitation and Sepharose Q chromatography
-
recombinant enzyme from Escherichia coli by heat denaturation
-
recombinant from Escherichia coli
recombinant His-tagged enzyme 3.0fold from Escherichia coli by heat treatment and nickel affinity chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RP by nickel affinity chromatography and dialysis
recombinant PFTA 32.6fold from Escherichia coli
-
of the recombinant protein
additional information
-