3.2.1.54: cyclomaltodextrinase
This is an abbreviated version!
For detailed information about cyclomaltodextrinase, go to the full flat file.
Reaction
Cyclomaltodextrin +
H2O=
linear maltodextrin
Synonyms
AfCda13, AglB, alpha-amylase, alpha-amylase II, CD-/pullulan-hydrolyzing enzyme, CD-ase, CD-degrading enzyme, CD-hydrolyzing amylase, CDA, CDase, CDase I-5, CMD, cyclodextrinase, cycloheptaglucanase, cyclohexaglucanase, cyclomaltodextrin dextrin-hydrolase, Cyclomaltodextrin hydrolase, decycling, cyclomaltodextrinase, CymH, cytoplasmic decycling maltodextrinase, EC 3.2.1.12, Env cda13A, FspCMD, H-17 CDase, H-17 thermostable CDase, LLCD, LsCda13, Lsp26X-Mdase, maltodextrin glucosidase, More, neopullulanase, PFTA, RA.04, thermophilic CDase, TK1770, TVA II
ECTree
KM Value
KM Value on EC 3.2.1.54 - cyclomaltodextrinase
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21
2-O-alpha-maltosylglucose
-
-
17
4-O-alpha-D-glucosylsucrose
-
-
0.26 - 50
alpha-cyclodextrin
0.244 - 154
beta-cyclodextrin
1.43 - 8.68
beta-cyclomaltodextrin
0.008 - 5.12
gamma-cyclodextrin
0.23 - 1.72
p-nitrophenyl-D-maltopentaoside
5.5
Phenyl alpha-maltoside
-
-
3.01 - 11
soluble starch
-
additional information
beta-cyclodextrin
11.1
acarbose
-
pH 4.5, 90°C
11.1
acarbose
-
pH 4.5, 90°C, recombinant enzyme
0.26
alpha-cyclodextrin
-
at 80°C, pH 4.5
0.28
alpha-cyclodextrin
pH 6.5, 55°C
0.93
alpha-cyclodextrin
-
-
1.23
alpha-cyclodextrin
-
-
1.79
alpha-cyclodextrin
-
-
2.5
alpha-cyclodextrin
-
-
2.61
alpha-cyclodextrin
-
pH 4.5, 90°C
2.61
alpha-cyclodextrin
-
pH 4.5, 90°C, recombinant enzyme
2.62
alpha-cyclodextrin
-
-
3.2
alpha-cyclodextrin
-
-
4.1
alpha-cyclodextrin
wild-type
4.4
alpha-cyclodextrin
-
-
5.66
alpha-cyclodextrin
-
pH 7.0, 40°C
7.9
alpha-cyclodextrin
-
pH 6, 55°C
10
alpha-cyclodextrin
-
-
50
alpha-cyclodextrin
-
-
0.244
beta-cyclodextrin
-
-
0.32
beta-cyclodextrin
pH 6.5, 55°C
0.454
beta-cyclodextrin
-
pH 7.0
0.63
beta-cyclodextrin
-
at 80°C, pH 4.5
0.83
beta-cyclodextrin
-
-
0.889
beta-cyclodextrin
-
pH 6.0
1.67
beta-cyclodextrin
-
-
1.8
beta-cyclodextrin
-
-
2
beta-cyclodextrin
-
pH 4.5, 85°C, mutant enzyme M439W/D440H
2.1
beta-cyclodextrin
-
-
2.1
beta-cyclodextrin
at pH 7.5 and 40°C
2.16
beta-cyclodextrin
-
pH 4.5, 90°C
2.16
beta-cyclodextrin
-
pH 4.5, 90°C, recombinant enzyme
2.47
beta-cyclodextrin
-
pH 7.0, 40°C
2.5
beta-cyclodextrin
-
-
2.65
beta-cyclodextrin
-
-
2.8
beta-cyclodextrin
-
-
2.9
beta-cyclodextrin
wild-type
3.6
beta-cyclodextrin
-
isoenzyme ag1B(1Met)
3.9
beta-cyclodextrin
-
-
3.9
beta-cyclodextrin
-
-
3.9
beta-cyclodextrin
-
isoenzyme ag1B*
4
beta-cyclodextrin
-
pH 4.5, 85°C, mutant enzyme D440H
6
beta-cyclodextrin
-
pH 4.5, 85°C, wild-type enzyme
6.06
beta-cyclodextrin
-
isoenzyme ag1B(2Met)
6.1
beta-cyclodextrin
-
-
8.78
beta-cyclodextrin
pH 4.5, 85°C, wild-type enzyme
12
beta-cyclodextrin
-
pH 6, 55°C
154
beta-cyclodextrin
pH 4.5, 85°C, mutant enzyme G415E
1.43
beta-cyclomaltodextrin
-
pH 6.0, dimer
8.68
beta-cyclomaltodextrin
-
pH 7.0, dodecamer
0.008
gamma-cyclodextrin
-
0.02
gamma-cyclodextrin
-
at 80°C, pH 4.5
0.47
gamma-cyclodextrin
-
-
0.8
gamma-cyclodextrin
-
-
0.92
gamma-cyclodextrin
-
-
1.2
gamma-cyclodextrin
-
-
1.3
gamma-cyclodextrin
-
-
2.2
gamma-cyclodextrin
wild-type
2.5
gamma-cyclodextrin
-
-
2.9
gamma-cyclodextrin
-
-
3.1
gamma-cyclodextrin
-
pH 7.0, 40°C
4.2
gamma-cyclodextrin
-
-
5.12
gamma-cyclodextrin
-
pH 4.5, 90°C
5.12
gamma-cyclodextrin
-
pH 4.5, 90°C, recombinant enzyme
0.8
maltoheptaose
-
-
1.8
maltoheptaose
-
pH 4.5, 85°C, mutant enzyme M439W/D440H
9.07
maltoheptaose
pH 4.5, 85°C, wild-type enzyme
9.77
maltoheptaose
pH 4.5, 85°C, mutant enzyme G415E
13
maltoheptaose
-
pH 4.5, 85°C, mutant enzyme D440H
14
maltoheptaose
-
pH 4.5, 85°C, wild-type enzyme
1.3
maltohexaose
-
-
1.2
maltopentaose
-
-
4
maltotetraose
-
-
4.5
maltotriose
-
-
62.9
maltotriose
-
pH 4.5, 90°C
62.9
maltotriose
-
pH 4.5, 90°C, recombinant enzyme
0.23
p-nitrophenyl-D-maltopentaoside
-
isoenzyme ag1B*
0.27
p-nitrophenyl-D-maltopentaoside
-
isoenzyme ag1B(1Met)
1.72
p-nitrophenyl-D-maltopentaoside
-
isoenzyme ag1B(2Met)
0.57
pullulan
-
mutant Y374A
1.4
pullulan
-
wild-type enzyme
3.01
soluble starch
-
-
-
5
soluble starch
-
pH 6, 55°C
-
11
soluble starch
pH 6.5, 55°C
-
0.19
starch
-
mutant Y374A
0.23
starch
-
wild-type enzyme
0.52
starch
-
pH 4.5, 90°C, recombinant enzyme
additional information
beta-cyclodextrin
KM-value is 2.94 mg/ml at pH 7.5 and 65°C
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetic study
-
3.2.1.54 cyclomaltodextrinase
results (
results (
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