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3.2.1.51: alpha-L-fucosidase

This is an abbreviated version!
For detailed information about alpha-L-fucosidase, go to the full flat file.

Word Map on EC 3.2.1.51

Reaction

an alpha-L-fucoside
+
H2O
=
L-fucose
 +
an alcohol

Synonyms

acid hydrolase, Afc2, Afc3, AFU, Alf1_Wf, alfA, alfB, alfC, alpha-fuc, alpha-fucosidase, alpha-L fucosidase, alpha-L-fucosidase, alpha-L-fucosidase 1, alpha-L-fucosidase 2, alpha-L-fucosidase I, alpha-L-fucosidase iso2, alpha-L-fucosidase isoenzyme 1, Alpha-L-fucoside fucohydrolase, alpha-L-fucoside fucohydrolase 2, alphafuc, bfo_2737, BgFUC, Eo0918, Eo3066, Eo3812, FpFucA, FucA, Fuca1, Fuca2, Fuca3, FucFA, fucosidase, alpha-L-1, tissue, h-Fuc, mfuc1, mfuc2, mfuc3, mfuc4, mfuc5, mfuc6, mfuc7, Pap-Alf, serum AFU, serum alpha-L-fucosidase, TfFuc1, TMalphafuc

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.51 alpha-L-fucosidase

Storage Stability

Storage Stability on EC 3.2.1.51 - alpha-L-fucosidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, 3-6% loss of activity after 3 days
-
-20°C, 0.5 mg/ml bovine serum albumin, 6 months
-
-20°C, acetate buffer, pH 6.0, 100 mM L-fucose, several months
-
-20°C, fully active after 29 days, 0-4°C, 10 mM Na2HPO4, pH 5.5, 0.02% NaN3, w/v, 100 mM L-fucose, 163 days
-
-20°C, in the presence of 1% fucose and presence or absence of 0.015% Triton X-100, 100% activity after 2 weeks
-
-20°C, pH 5.5 in presence of 1% L-fucose, 100% activity after 6 weeks
-
2-4°C, 29 days
-
2-4°C, in the presence of 1% fucose and 0.015% Triton X-100, 100% activity after 3 months
-
2-4°C, pH 5.5 in presence of 1% L-fucose, 77% activity after 6 weeks
-
2-4°C, stable for one month
-
21°C, pH 5.5 in presence of 1% L-fucose, 100% activity after 3 days
-
30°C, 30% loss of activity after 40 days
-
37°C, 5% CO2, ambient air, pH 7.0, 24 h loss of activity and increase of the pH-value, membrane associated enzyme
-
37°C, 5% CO2, high humidity, 24 h, 70% loss of activity, enzyme in semen from storage in liquid nitrogen
-
37°C, 5% CO2, high humidity, 24 h, 90% loss of activity, enzyme in seminal plasma
-
37°C, 5% CO2, high humidity, 72 h, no activity, enzyme in seminal plasma
-
37°C, 5% CO2, high humidity, pH 7.0, 24 h, 20% loss of activity, membrane associated enzyme
-
37°C, 5% CO2, high humidity, pH 7.0, 72 h, 30% loss of activity, membrane associated enzyme
-
4°C, 0.2 M sodium citrate, pH 6.0, 20% polyethylene glycol, 2 months
4°C, 10 mM sodium phosphate, pH 5.5, 1 mM EDTA, 1 mM PMSF, one month
-
4°C, 24 h, approximate 78% of initial activity is retained
4°C, 3 weeks, enzyme retains 100% of its activity when stored in presence of 20 mM CHAPS
-
4°C, enzyme freeze dried from aqueous solution containing 3 mM DTT and 5-20 mg/ml bovine serum albumin retains full activity for at least one year
-
4°C, in the presencen or absence of 50% glycerol, 0.05mg/ml bovine serum albumine or 2 mM beta-mercaptoethanol, enzyme from epimastigote extracts and from NaCl-solubilized membrane fractions are unstable
-
55°C, LB medium, 4 h
60°C, 40% loss of activity after 1 day
-
frozen, 1 year
-
frozen, more than 90% of its initial activity after 3 months
-