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3.2.1.50: alpha-N-acetylglucosaminidase

This is an abbreviated version!
For detailed information about alpha-N-acetylglucosaminidase, go to the full flat file.

Word Map on EC 3.2.1.50

Reaction

(GlcNAcalpha(1-4))n
+
H2O
=
(GlcNAcalpha(1-4))n-1
+
2-(acetylamino)-2-deoxy-beta-D-glucopyranose

Synonyms

2-acetamido-2-deoxy-alpha-D-glucoside acetamidodeoxyglucohydrolase, AgnB, AgnC, alpha-acetylglucosaminidase, alpha-N-acetylglucosaminidase, BMN 250, exo-glycosidase, glycosidase, heparan N-sulfatase, N-acetyl-alpha-D-glucosaminidase, N-acetyl-alpha-glucosaminidase, NAG, NAGase, NAGLU, Sanfilippo b corrective factor

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.50 alpha-N-acetylglucosaminidase

KM Value

KM Value on EC 3.2.1.50 - alpha-N-acetylglucosaminidase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.74
2,4-dinitrophenyl-alpha-N-acetyl-D-glucosaminide
pH 7.3
0.043 - 0.61
2-nitrophenyl alpha-N-acetylglucosaminide
-
form I 0.067, form II 0.043
-
1.07 - 5.34
4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside
1.1
4-nitrophenyl-alpha-N-acetyl-D-glucosaminide
pH 7.3
4.3 - 7.8
4-nitrophenyl-N-acetyl-alpha-D-glucosaminide
0.00065
chitobiose
-
-
0.003
chitotetraose
-
-
0.0008
chitotriose
-
-
0.13 - 0.22
methylumbelliferyl-N-acetyl-alpha-D-glucosaminide
0.001
methylumbelliferyl-N-acetylglucosamine
-
-
0.0035
methylumbelliferyl-N-acetylglucosamine-6-sulfate
-
-
0.2 - 0.28
N-acetyl-alpha-D-Gluc-1,4-(1-methoxyphenyl)Gal
0.0166
O-(alpha-acetamido-2-deoxy-D-glucopyranosyl)-(1-3)-[L-6,3H]idoronic acid
-
pH 4.5, 37°C, with bovine serum albumin
0.043 - 0.61
o-nitrophenyl-alpha-N-acetylglucosaminide
0.00055
p-nitrophenyl-1-thio-N-acetylglucosamine
-
-
0.13 - 0.3
p-nitrophenyl-alpha-N-acetylglucosaminide
0.12 - 1.6
phenyl-alpha-N-acetylglucosaminide
0.39
UDP-N-acetylglucosamine
-
0.58 in the presence of 0.025% bovine serum albumin