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3.2.1.48: sucrose alpha-glucosidase

This is an abbreviated version!
For detailed information about sucrose alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.48

Reaction

isomaltose
+
H2O
= 2 D-glucose

Synonyms

alpha-glucosidase, glucosidase, sucrose alpha-, intestinal sucrase, isomaltase, More, PF0132, pro-SI, SI, sucrase, sucrase isomaltase, sucrase-invertase, sucrase-isomaltase, sucrase-isomaltase enzyme complex, sucrase/isomaltase, sucrose alpha-glucohydrolase, sucrose hydrolase, SUH

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.48 sucrose alpha-glucosidase

Engineering

Engineering on EC 3.2.1.48 - sucrose alpha-glucosidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C1229Y
-
heterozygous mutation within the sucrose domain, found in patients with congenital sucrase-isomaltase deficiency. Recombinant mutant protein is transported only to the Golgi apparatus. Isomaltase activity is not affected by the mutation
D1394E
D1500E
site-directed mutagenesis of a catalytic residue, the mutant shows reduced maltase activity compared to wild-type
D1500N
D1500S
site-directed mutagenesis of a catalytic residue, the mutant shows reduced maltase activity compared to wild-type
D1500Y
D1700S
about 95% decrease in hydrolysis of sucrose, about 40% decrease in hydrolysis of maltose, about 30% increase in hydrolysis of isomaltulose
D505E
D604E
site-directed mutagenesis of a catalytic residue, the mutant shows reduced maltase activity compared to wild-type
D604N
D604S
D604Y
F1093A/F1095A/F1099A
-
site-directed mutagenesis, mutation of the extracellular folding signal motif, CSID-phenotype II-like temperature-sensitive mutant enzyme which undergoes transport arrest in the endoplasmic reticulum/cis-Golgi intermediate and cis-Golgi compartments and acquires correct folding and function at reduced temperatures as a consequence of anterograde and retrograde transport between endoplasmic reticulum and cis-Golgi, overview
F1745C
-
heterozygous mutation within the sucrose domain, found in patients with congenital sucrase-isomaltase deficiency. Recombinant mutant protein is misfolded and can not exit the endoplasmic reticulum. Isomaltase activity is not affected by the mutation
G1073D
-
heterozygous mutation found in patients with congenital sucrase-isomaltase deficiency. Recombinant mutant protein is misfolded and can not exit the endoplasmic reticulum
Q1098P
V15F
35% reduced enzymatic activity in vitro compared with wild-type enzyme. The mutation is detected in 6/7 sequenced familial cases of congenital sucrase-isomaltase deficiency
V577G
-
heterozygous mutation found in patients with congenital sucrase-isomaltase deficiency. Recombinant mutant protein is misfolded and can not exit the endoplasmic reticulum
E322Q
site-directed mutagenesis, a catalytically inactive Xag SUH mutant
G219R
site-directed mutagenesis, the mutant shows increased catalytic activity compared to the wild-type enzyme
G219R/G444R
site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme
G219R/L414R
site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme
G444R
site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme
L414R
site-directed mutagenesis, the mutant shows slightly reduced catalytic activity compared to the wild-type enzyme
L414R/G444R
site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme
additional information