3.2.1.41: pullulanase

This is an abbreviated version!
For detailed information about pullulanase, go to the full flat file.

Reaction

Synonyms

1,4-alpha-D-glucan glucanohydrolase, Alpha-dextrin endo-1,6-alpha-glucosidase, amylase-pullulanase, amylopectin 6-glucanohydrolase, amylopullulanase, AmyX, Apu, ApuK885, ApuM957, Ask, BaPul13A, BDPulA324, BmPul, BNPulA324, CAC60157, debranching enzyme, EC 3.2.1.69, glucanohydrolase, amylopectin 6-, limit dextrinase, More, Nostoc punctiforme debranching enzyme, NPDE, PbPulA, Pcal-1616, PelBsp-PulA, PfAPU, Promozyme 200 L, PUL US105, Pul13A, Pul3YH5, PulA, PulASK, PulB, pulGT, Pullulan 6-glucanohydrolase, pullulan-6-glucanohydrolase, pullulanase, pullulanase type 1, pullulanase type I, pullulanase type II, PulWB42, R-enzyme, Saci_1162, TPApu, TTC1198, TTHpu, type II pullulanase, ZUP1

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.41 pullulanase

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Results	in table
35	Activating Compound
6506	AA Sequence
20	Application
1	CAS Registry Number
38	Cloned(Commentary)
10	Crystallization (Commentary)
1	Expression
7	General Information
2	General Stability
233	Inhibitors
8	kcat/KM [mM/s]
50	KM Value [mM]
45	Localization
89	Metals/Ions
73	Molecular Weight [Da]
97	Natural Substrates/ Products (Substrates)
11	Organic Solvent Stability
437	Organism
2	Pathway
44	PDB ID
72	pH Optimum
36	pH Range
28	pH Stability
8	pI Value
11	Posttranslational Modification
47	Protein Variants
63	Purification (Commentary)
1	Reaction
9	Reaction Type
139	Reference
25	Source Tissue
26	Specific Activity [micromol/min/mg]
2	Storage Stability
410	Substrates and Products (Substrate)
57	Subunits
145	Synonyms
1	Systematic Name
70	Temperature Optimum [°C]
36	Temperature Range [°C]
81	Temperature Stability [°C]
39	Turnover Number [1/s]

pH Stability

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pH Stability on EC 3.2.1.41 - pullulanase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

3 - 11

2 entries

3 - 5

Thermoanaerobacter thermohydrosulfuricus

-

-

136406

3.5 - 8

Thermus caldophilus

-

stable at 4°C for 24 h

136435

4 - 10

Exiguobacterium acetylicum

I3WU34

30 min, more than 93% residual activity

738951

4 - 11

Bacillus sp. (in: Bacteria)

-

stable at 4°C for 24 h

136431

4 - 8.5

Bacillus acidopullulyticus

-

-

136405

4.5 - 10.5

Thermoanaerobacter thermohydrosulfuricus

-

-

136410

4.5 - 12

Klebsiella aerogenes

-

-

136423

4.5 - 5.5

Thermoanaerobacter thermohydrosulfuricus

-

-

136414

5

Geobacillus thermocatenulatus

A0A1V0FWX7

4°C, 60 min, 75% residual activity, wild-type enzyme

750894

5 - 10

Micrococcus sp.

-

10% loss of activity in the presence of CaCl2

136440

5 - 5.5

Thermoanaerobacter ethanolicus

-

-

654227

5 - 6

Anoxybacillus sp.

EPZ37738

-

737518

5 - 6.5

Thermococcus siculi

B6SED6

after incubation at 95°C for 4 h in a pH range from 5.0 to 6.5, the residual pullulanase and amylase activities of the enzyme are above 90% of the maximum activity

714788

5 - 8

Niallia circulans

-

-

136401

5 - 9

Bacillus cereus

-

-

136419

5.5 - 10.5

Paenibacillus barengoltzii

A0A0C5GWS2

30 min, enzyme retains more than 80% of its activity

752096

5.5 - 8

Oryza sativa

-

at 30°C

695305

6

2 entries

6 - 8

Thermus thermophilus

Q72ID4

1 h, 70°C, stable

749230

6 - 8.5

Geobacillus stearothermophilus

-

-

136403

6.5 - 11

Bacillus sp. (in: Bacteria)

-

-

136421

6.5 - 8.5

Priestia megaterium

A0A173DUJ5

30 min, enzyme retains more than 85% of its original activity

750159

7

Geobacillus thermocatenulatus

A0A1V0FWX7

4°C, 60 min, 85% residual activity, wild-type enzyme

750894

8

Geobacillus thermocatenulatus

A0A1V0FWX7

4°C, 60 min, 70% residual activity, wild-type enzyme

750894

9

Geobacillus thermocatenulatus

A0A1V0FWX7

4°C, 60 min, 55% residual activity, wild-type enzyme

750894

3 - 11

Klebsiella aerogenes

-

-

136430

3 - 11

Klebsiella pneumoniae

-

-

136430

6

Bacillus acidopullulyticus

-

91% loss of activity at 60°C for 30 min

136438

6

Geobacillus thermocatenulatus

A0A1V0FWX7

4°C, 60 min, 90% residual activity, wild-type enzyme

750894