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3.2.1.41: pullulanase

This is an abbreviated version!
For detailed information about pullulanase, go to the full flat file.

Word Map on EC 3.2.1.41

Reaction

(Glcalpha(1-4)Glcalpha(1-4)Glcalpha(1-6))n
+
H2O
=
alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose
+
(Glcalpha(1-4)Glcalpha(1-4)Glcalpha(1-6))n-1

Synonyms

1,4-alpha-D-glucan glucanohydrolase, Alpha-dextrin endo-1,6-alpha-glucosidase, amylase-pullulanase, amylopectin 6-glucanohydrolase, amylopullulanase, AmyX, Apu, ApuK885, ApuM957, Ask, BaPul13A, BDPulA324, BmPul, BNPulA324, CAC60157, debranching enzyme, EC 3.2.1.69, glucanohydrolase, amylopectin 6-, limit dextrinase, More, Nostoc punctiforme debranching enzyme, NPDE, PbPulA, Pcal-1616, PelBsp-PulA, PfAPU, Promozyme 200 L, PUL US105, Pul13A, Pul3YH5, PulA, PulASK, PulB, pulGT, Pullulan 6-glucanohydrolase, pullulan-6-glucanohydrolase, pullulanase, pullulanase type 1, pullulanase type I, pullulanase type II, PulWB42, R-enzyme, Saci_1162, TPApu, TTC1198, TTHpu, type II pullulanase, ZUP1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.41 pullulanase

pH Range

pH Range on EC 3.2.1.41 - pullulanase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 5
pH 2.0: about 60% of maximal activity, pH 5.0: about 60% of maximal activity, , amylolytic activity toward starch
4
-
not active below, maltose + cyclomaltooctaose
4 - 7.5
4.3 - 7.2
pH 4.3: about 80% of maximal activity, pH 7.2: about 75% of maximal activity
4.5 - 6.5
-
over 89% of maximal activity between pH 5.0 and pH 6.5, over 75% at pH 4.5
4.5 - 7.8
5 - 10
-
-
5 - 11
more than 60% of maximum activity
5 - 7
5 - 8
5 - 8.5
pH 5.0: about 65% of maximal activity, pH 8.5: about 65% of maximal activity
5 - 9
pH 5.0: about 80% of maximal activity, pH 9.0: about 35% of maximal activity, wild-type enzyme
5.5 - 8.5
-
pH 5.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity
6 - 9
pH 6.0: about 45% of maximal activity, pH 9.0: about 25% of maximal activity