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3.2.1.36: hyaluronoglucuronidase

This is an abbreviated version!
For detailed information about hyaluronoglucuronidase, go to the full flat file.

Word Map on EC 3.2.1.36

Reaction

hyaluronic acid hexasaccharide
+
H2O
=
GlcAbeta(1-3)GlcNAcbeta(1-4)GlcA
+
GlcNAcbeta(1-4)GlcAbeta(1-3)GlcNAc

Synonyms

glucuronoglucosaminoglucan hyaluronate lyase, HAase, HAase-B, hyaluronate lyase HylP2, hyaluronate lyase HylP3, hyaluronidase, HYase, LHase, orgelase, VesT2a, VesT2b

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.36 hyaluronoglucuronidase

Purification

Purification on EC 3.2.1.36 - hyaluronoglucuronidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Superdex 200 gel filtration
-
highly purified, chromatography steps
-
Ni2+ chelate chromatography, gel filtration
Ni2+-affinity chromatography, gel filtration
to homogeneity, chromatography steps, isoelectric focusing