3.2.1.33: amylo-alpha-1,6-glucosidase

This is an abbreviated version!
For detailed information about amylo-alpha-1,6-glucosidase, go to the full flat file.

Reaction

Synonyms

1,4-glucan-4-glucosyltransferase/amylo-1,6-glucosidase, 4-alpha-glucano-transferase amylo-1,6-glucosidase, alpha-(1,6)-glucosidase, alpha-1,6-glucosidase, amylo-1,6-glucosidase, amylo-1,6-glucosidase-oligo-1,4-1,4-transferase, amylo-1,6-glucosidase/1,4-alpha-glucan 4-alpha-glucan 4-alpha-glycosyltransferase, amylo-1,6-glucosidase/1,4-alpha-glucan:1,4-alpha-glucan 4-alpha-glycosyltransferase, amylo-1,6-glucosidase/4-alpha-glucanotransferase, amylo-1,6-glucosidase/oligo-1,4-1,4-glucantransferase, amylo-alpha-1,6-glucosidase, amylo-alpha-1,6-glucosidase activity, amyloglucosidase, amylopectin 1,6-glucosidase, debrancher enzyme, debrancher protein, dextrin 6-alpha-D-glucosidase, dextrin 6-alpha-glucosidase, dextrin 6-glucanohydrolase, dextrin 6-glucohydrolase, dextrin-1,6-glucosidase, dextrin:6-glucohydrolase, GDE, GDE amylo-alpha-1,6-glucosidase, glucosidase, amylo-1,6-, glucosidase/transferase, glycogen debranching enzyme, glycogen debranching system, glycogen phosphorylase dextrin 6-glucanohydrolase, glycogen phosphorylase limit dextrin alpha-1,6-glucohydrolase, glycogen phosphorylase limit dextrin debranching system, indirect debranching enzyme, NPDE, oligo-alpha-1,4-glucan:alpha-1,4-glucan-4-glycosyltransferase-amylo-1,6-glucosidase, SSO2094, transferase-glucosidase, TreX

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.33 amylo-alpha-1,6-glucosidase

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Results	in table
14	Activating Compound
2306	AA Sequence
6	Application
1	CAS Registry Number
8	Cloned(Commentary)
4	Crystallization (Commentary)
216	Disease/ Diagnostics
4	General Information
5	General Stability
6	IC50 Value
105	Inhibitors
17	kcat/KM [mM/s]
1	Ki Value [mM]
38	KM Value [mM]
4	Localization
7	Metals/Ions
54	Molecular Weight [Da]
26	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
84	Organism
3	Pathway
12	PDB ID
36	pH Optimum
6	pH Range
1	pH Stability
1	Posttranslational Modification
11	Protein Variants
50	Purification (Commentary)
14	Reaction
1	Reaction Type
80	Reference
82	Source Tissue
39	Specific Activity [micromol/min/mg]
8	Storage Stability
283	Substrates and Products (Substrate)
32	Subunits
111	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
9	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.2.1.33 - amylo-alpha-1,6-glucosidase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

0°C, 50 mM glycerophosphate, 2 mM EDTA, 1 mM dithiothreitol, pH 7.0, 2 months, without loss of activity

Oryctolagus cuniculus

-

171388

4°C, 50 mM glycerol-1-phosphate, 2 mM EDTA, 1 mM dithiothreitol, pH 7

Gallus gallus

-

171362

4°C, 50 mM glycerol-1-phosphate, 2 mM EDTA, 1 mM dithiothreitol, pH 7.0, 1 month, little or no loss of activity

Oryctolagus cuniculus

-

171387

4°C, 50 mM Tris, 5 mM EDTA, 1 mM dithiothreitol, pH 7.2

Oryctolagus cuniculus

-

171361, 171386

5°C, crystalline suspension, ammonium sulfate, retains activity for many months or years

Oryctolagus cuniculus

-

3247

frozen, concentrated solution without salt, retains activity for many months or years

Oryctolagus cuniculus

-

3247

frozen, solution in Tris-glycerol buffer, 3 months, without loss of activity

Squalus acanthias

-

171384