3.2.1.33: amylo-alpha-1,6-glucosidase This is an abbreviated version! For detailed information about amylo-alpha-1,6-glucosidase, go to the full flat file .
Reaction
6-O-alpha-D-glucosyl cyclomaltoheptaose +
H2O =
D-glucose +
cyclomaltoheptaose
Synonyms 1,4-glucan-4-glucosyltransferase/amylo-1,6-glucosidase, 4-alpha-glucano-transferase amylo-1,6-glucosidase, alpha-(1,6)-glucosidase, alpha-1,6-glucosidase, amylo-1,6-glucosidase, amylo-1,6-glucosidase-oligo-1,4-1,4-transferase, amylo-1,6-glucosidase/1,4-alpha-glucan 4-alpha-glucan 4-alpha-glycosyltransferase, amylo-1,6-glucosidase/1,4-alpha-glucan:1,4-alpha-glucan 4-alpha-glycosyltransferase, amylo-1,6-glucosidase/4-alpha-glucanotransferase, amylo-1,6-glucosidase/oligo-1,4-1,4-glucantransferase, amylo-alpha-1,6-glucosidase, amylo-alpha-1,6-glucosidase activity, amyloglucosidase, amylopectin 1,6-glucosidase, debrancher enzyme, debrancher protein, dextrin 6-alpha-D-glucosidase, dextrin 6-alpha-glucosidase, dextrin 6-glucanohydrolase, dextrin 6-glucohydrolase, dextrin-1,6-glucosidase, dextrin:6-glucohydrolase, GDE, GDE amylo-alpha-1,6-glucosidase, glucosidase, amylo-1,6-, glucosidase/transferase, glycogen debranching enzyme, glycogen debranching system, glycogen phosphorylase dextrin 6-glucanohydrolase, glycogen phosphorylase limit dextrin alpha-1,6-glucohydrolase, glycogen phosphorylase limit dextrin debranching system, indirect debranching enzyme, NPDE, oligo-alpha-1,4-glucan:alpha-1,4-glucan-4-glycosyltransferase-amylo-1,6-glucosidase, SSO2094, transferase-glucosidase, TreX
ECTree
KM Value
KM Value on EC 3.2.1.33 - amylo-alpha-1,6-glucosidase
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15.9 - 19.3
6-O-alpha-D-glucosyl cyclomaltoheptaose
12.23
6-O-alpha-maltosyl-beta-cyclodextrin
-
at 70°C in 50 mM sodium acetate buffer (pH 5.5)
0.206
6-O-alpha-maltotetraosyl-beta-cyclodextrin
pH 5.5, 75°C
1.8 - 4.45
63-alpha-glucosyl maltotetraose
5.6
63-alpha-maltotriosyl maltotetraose
-
branched heptasaccharide B7, muscle enzyme
2.8 - 8.1
alpha-glucosyl fluoride
4.3 - 11
amylopectin beta-dextrin
-
3.8 - 7.2
beta-amylase limit dextrin
-
0.063 - 1.6
glycogen phosphorylase limit dextrin
-
0.64
maltodecaose
-
in 50 mM sodium phosphate buffer (pH 7.5) at 30°C
0.62
maltododecaose
-
in 50 mM sodium phosphate buffer (pH 7.5) at 30°C
0.81
maltononaose
-
in 50 mM sodium phosphate buffer (pH 7.5) at 30°C
0.51
maltoundecaose
-
in 50 mM sodium phosphate buffer (pH 7.5) at 30°C
additional information
amylopectin
15.9
6-O-alpha-D-glucosyl cyclomaltoheptaose
-
mutant E564Q, pH 6.5, 37°C
16.3
6-O-alpha-D-glucosyl cyclomaltoheptaose
-
wild type, pH 6.5, 37°C
16.6
6-O-alpha-D-glucosyl cyclomaltoheptaose
-
mutant D535N, pH 6.5, 37°C
19.3
6-O-alpha-D-glucosyl cyclomaltoheptaose
-
mutant D670N, pH 6.5, 37°C
1.8
63-alpha-glucosyl maltotetraose
-
branched pentasaccharide B5, pH 6.6
4.45
63-alpha-glucosyl maltotetraose
-
branched pentasaccharide "fast B5", muscle enzyme
2.8
alpha-glucosyl fluoride
-
-
8.1
alpha-glucosyl fluoride
-
with 9.6% glycogen
4.3
amylopectin beta-dextrin
-
-
-
11
amylopectin beta-dextrin
-
-
-
3.8
beta-amylase limit dextrin
-
beta-dextrin from shellfish glycogen
-
7.2
beta-amylase limit dextrin
-
beta-dextrin from shellfish glycogen
-
32
D-glucose
-
in the presence of glycogen
0.063
glycogen phosphorylase limit dextrin
-
-
-
0.063
glycogen phosphorylase limit dextrin
-
phi-dextrin
-
0.74
glycogen phosphorylase limit dextrin
-
-
-
0.74
glycogen phosphorylase limit dextrin
-
phi-dextrin from shellfish glycogen
-
1.6
glycogen phosphorylase limit dextrin
-
phi-dextrin from shellfish glycogen
-
additional information
amylopectin
Km-values: 31.1 mg/ml at pH 5.5, 75°C, in absence of DMSO. 88.0 mg/ml at pH 5.5, 75°C, in presence of DMSO. 92.3 mg/ml at pH 6.5, 75°C, in absence of DMSO. 345 mg/ml at pH 5.5, 75°C, in presence of DMSO
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kinetic mechanism
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