3.2.1.24: alpha-mannosidase

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For detailed information about alpha-mannosidase, go to the full flat file.

Reaction

Synonyms

1,2-alpha-D-mannosidase, 1,2-alpha-mannosidase, acid alpha-mannosidase, acidic alpha-mannosidase, alpha 1,2-mannosidase, Alpha mannosidase 6A8B, alpha-1,2-mannosidase, alpha-1,3 mannosidase, alpha-1,4 mannosidase, alpha-D-mannopyranosidase, alpha-D-mannosidase, Alpha-D-mannoside mannohydrolase, alpha-man, alpha-mann, alpha-mannosidase, alpha-mannosidase C, alpha-mannosidase E-II, alpha-mannosidase I, alpha-mannosidase IA, alpha-mannosidase II, alpha-mannosidase III, alpha1,2-mannosidase, AMAN, class II alpha-mannosidase, CpGH125, ER-alpha-mannosidase, ER-mannosidase II, exo-alpha-mannosidase, GH38 alpha-mannosidase, GH38 alpha-mannosidase II, GH38 class II alpha-mannosidase, GH38 enzyme, Golgi alpha-mannosidase II, Golgi mannosidase IA, Golgi mannosidase IB, Laman, Lysosomal acid alpha-mannosidase, lysosomal alpha-D-mannosidase, lysosomal alpha-mannosidase, Man1p, MAN2B1, Man2C1, Man2C1 alpha-mannosidase, ManA, mannanase, MGG_00994.6, More, neutral alpha-mannosidase, neutral/cytosol mannosidase, p-nitrophenyl-alpha-mannosidase, SpGH125, SpGH38, Ssa-man, SSO3006, TM1851, TMM, velmanase alfa

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.24 alpha-mannosidase

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Results	in table
24	Activating Compound
3495	AA Sequence
7	Application
1	CAS Registry Number
20	Cloned(Commentary)
10	Crystallization (Commentary)
1134	Disease/ Diagnostics
1	Expression
9	General Information
13	General Stability
32	IC50 Value
334	Inhibitors
46	kcat/KM [mM/s]
57	Ki Value [mM]
113	KM Value [mM]
58	Localization
76	Metals/Ions
91	Molecular Weight [Da]
116	Natural Substrates/ Products (Substrates)
339	Organism
2	Oxidation Stability
3	Pathway
38	PDB ID
89	pH Optimum
14	pH Range
20	pH Stability
24	Posttranslational Modification
56	Protein Variants
84	Purification (Commentary)
5	Reaction
4	Reaction Type
135	Reference
1	Renatured (Commentary)
104	Source Tissue
54	Specific Activity [micromol/min/mg]
12	Storage Stability
317	Substrates and Products (Substrate)
38	Subunits
104	Synonyms
1	Systematic Name
30	Temperature Optimum [°C]
5	Temperature Range [°C]
27	Temperature Stability [°C]
36	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.2.1.24 - alpha-mannosidase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-10°C, several weeks

Aspergillus flavus

-

136009

-20°C, 0.2 mg protein/ml, 1 year

Rhodococcus erythropolis

-

135985

-20°C, 7, 10 and 30 days, 20%, 30% and 40% loss of activity, respectively

Equus caballus

-

730990

-20°C, pH 6.0, 50% glycerol, 3 months

Styela plicata

-

135995

0-4°C, pH 6.0, several weeks

Rattus sp.

-

135984

4°C or -20°C, 4 months

Aspergillus phoenicis

-

586942

4°C, 10 mM sodium acetate, pH 6.0, high protein concentration, 1 month

Rattus sp.

-

136006, 136007

4°C, 25 mM sodium phosphate, pH 7.0, 9 months

Helianthus annuus

-

135975

4°C, glutathione S-transferase/mannosidase III fusion protein is stable for at least 1 week

Spodoptera frugiperda

-

646644

4°C, pH 4.5-6, at least 6 months

Aspergillus oryzae

-

136000

5°C, 50% loss of activity after 3 weeks

Saccharomyces cerevisiae

-

136011