3.2.1.24: alpha-mannosidase
This is an abbreviated version!
For detailed information about alpha-mannosidase, go to the full flat file.
Word Map on EC 3.2.1.24
-
3.2.1.24
-
oligosaccharide
-
mannose
-
glycosidases
-
n-linked
-
swainsonine
-
hydrolases
-
mannans
-
n-glycans
-
concanavalin
-
alpha-fucosidase
-
xylanase
-
beta-n-acetylglucosaminidase
-
beta-d-galactosidase
-
beta-hexosaminidase
-
cellulase
-
endo-beta-n-acetylglucosaminidase
-
high-mannose
-
beta-d-glucosidase
-
galactomannans
-
1-deoxymannojirimycin
-
asparagine-linked
-
jack
-
beta-mannosidase
-
exoglycosidase
-
mannosylated
-
man9glcnac2
-
n-acetyl-beta-glucosaminidase
-
locust
-
beta-d-glucuronidase
-
complex-type
-
glucomannans
-
konjac
-
mannose-type
-
hemicellulases
-
glc3man9glcnac2
-
castanospermine
-
oligomannosides
-
alpha-d-galactosidase
-
alpha-d-glucosidase
-
mannose-rich
-
beta-xylosidase
-
lipid-linked
-
hexosaminidase
-
beta-n-acetylhexosaminidase
-
mannose-containing
-
hybrid-type
-
i-cell
-
mucolipidosis
-
oligomannose
-
analysis
-
cellulosome
-
diagnostics
-
medicine
- 3.2.1.24
- oligosaccharide
- mannose
- glycosidases
-
n-linked
- swainsonine
- hydrolases
- mannans
- n-glycans
-
concanavalin
- alpha-fucosidase
- xylanase
- beta-n-acetylglucosaminidase
- beta-d-galactosidase
- beta-hexosaminidase
- cellulase
-
endo-beta-n-acetylglucosaminidase
-
high-mannose
- beta-d-glucosidase
- galactomannans
- 1-deoxymannojirimycin
-
asparagine-linked
-
jack
- beta-mannosidase
-
exoglycosidase
-
mannosylated
- man9glcnac2
- n-acetyl-beta-glucosaminidase
-
locust
- beta-d-glucuronidase
-
complex-type
- glucomannans
-
konjac
-
mannose-type
-
hemicellulases
- glc3man9glcnac2
- castanospermine
-
oligomannosides
- alpha-d-galactosidase
- alpha-d-glucosidase
-
mannose-rich
- beta-xylosidase
-
lipid-linked
- hexosaminidase
- beta-n-acetylhexosaminidase
-
mannose-containing
-
hybrid-type
- i-cell
- mucolipidosis
-
oligomannose
- analysis
- cellulosome
- diagnostics
- medicine
Reaction
Synonyms
1,2-alpha-D-mannosidase, 1,2-alpha-mannosidase, acid alpha-mannosidase, acidic alpha-mannosidase, alpha 1,2-mannosidase, Alpha mannosidase 6A8B, alpha-1,2-mannosidase, alpha-1,3 mannosidase, alpha-1,4 mannosidase, alpha-D-mannopyranosidase, alpha-D-mannosidase, Alpha-D-mannoside mannohydrolase, alpha-man, alpha-mann, alpha-mannosidase, alpha-mannosidase C, alpha-mannosidase E-II, alpha-mannosidase I, alpha-mannosidase IA, alpha-mannosidase II, alpha-mannosidase III, alpha1,2-mannosidase, AMAN, class II alpha-mannosidase, CpGH125, ER-alpha-mannosidase, ER-mannosidase II, exo-alpha-mannosidase, GH38 alpha-mannosidase, GH38 alpha-mannosidase II, GH38 class II alpha-mannosidase, GH38 enzyme, Golgi alpha-mannosidase II, Golgi mannosidase IA, Golgi mannosidase IB, Laman, Lysosomal acid alpha-mannosidase, lysosomal alpha-D-mannosidase, lysosomal alpha-mannosidase, Man1p, MAN2B1, Man2C1, Man2C1 alpha-mannosidase, ManA, mannanase, MGG_00994.6, More, neutral alpha-mannosidase, neutral/cytosol mannosidase, p-nitrophenyl-alpha-mannosidase, SpGH125, SpGH38, Ssa-man, SSO3006, TM1851, TMM, velmanase alfa
ECTree
3.2.1.24 alpha-mannosidaseAdvanced search results
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results (Natural Substrates Products
Natural Substrates Products on EC 3.2.1.24 - alpha-mannosidase
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NATURAL SUBSTRATE 
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 Man9GlcNAc + 3 H2O
Man8GlcNAc + Man7GlcNAc + 3 alpha-D-mannopyranose
-
-
products from unbound fraction of cobalt-chelating Sepharose
?
2-O-alpha-D-mannotriose + H2O
alpha-D-mannopyranose + ?
-
highly specific for alpha-1,2-mannosidic linkages
-
?
4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 alpha-D-mannopyranose
-
specific cleavage of alpha-1,4- or alpha-1,6-linkages
-
?
alpha-(1-2)-mannobiose + H2O
alpha-D-mannopyranose + D-mannopyranose
-
-
-
?
alpha-(1-3)-mannobiose + H2O
alpha-D-mannopyranose + D-mannopyranose
-
highest activity and affinity
-
?
alpha-(1-6)-mannobiose
alpha-D-mannopyranose + D-mannopyranose
-
low activity
-
?
alpha-conglutin + H2O
alpha-D-mannopyranose + ?
-
lupin seed storage glycoprotein, not active to beta- or gamma-conglutin
-
?
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O
6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + H2O
alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc + alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)-beta-D-Man
-
cleaves asparagine-linked high mannose oligosaccharides to form the core Man3GlcNAc2
-
?
alpha-D-mannosidosis fibroblasts + H2O
Manbeta(1-4)GlcNAc + ?
-
-
?
Man-alpha-(1-2)-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]-Manalpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta(1-4)GlcNAc + H2O
?
-
-
-
-
?
Man3GlcNAc2 + H2O
Man2GlcNAc2 + alpha-D-mannopyranose
-
Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed
-
?
Man5-9GlcNAc + H2O
alpha-D-mannopyranose + ?
-
low activity for Man5GlcNAc
-
?
Man5GlcNAc2 + H2O
Man4GlcNAc2 + alpha-D-mannopyranose
-
cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages
mixture of three isomeric oligosaccharides
?
Man6 + H2O
alpha-D-mannose + ?
-
derived from ovalbumin, alpha-1,2: 1,3: 1,6-linkages, more than 10fold higher specific activity for isoform EII than for EI
-
?
Man8GlcNAc2 + 3 H2O
Man5GlcNAc2 + 3 D-mannose
-
Golgi alpha-mannosidase in vivo
-
-
?
Man9-5GlcNAc + 4 H2O
Man5-3GlcNAc + 4 alpha-D-mannopyranose
-
different product composition for alpha-mannosidases IA, IB and II
-
?
Man9GlcNAc + H2O
Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc + alpha-D-mannopyranose
-
Co2+ activated alpha-mannosidase
end products
?
Man9GlcNAc2 + H2O
Man5GlcNAc2 + Man6GlcNAc2 + Man7GlcNAc2 + Man8GlcNAc2 + alpha-D-mannopyranose
Man9GlcNAc2 + H2O
Man8GlcNAc2 + mannose
-
endoplasmic reticulum alpha-mannosidase in vivo
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNac-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manbeta(1-6)]ManbetaGlcNAc-beta-GlcNAc + H2O
alpha-D-mannopyranose + ?
-
-
-
?
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
-
-
-
?
Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
-
-
-
?
Manalpha(1-3)GlcNAc + H2O
alpha-D-mannopyranose + N-acetyl-D-glucosamine
-
-
-
?
Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
-
-
-
?
Manalpha(1-4)Manbeta(1-4)Man + H2O
alpha-D-mannopyranose + Manbeta(1-4)Man
-
-
-
?
Manalpha(1-6)GlcNAc + H2O
alpha-mannopyranose + N-acetyl-D-glucosamine
-
-
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
-
4 times faster hydrolysis than Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
-
-
-
?
mannosyl rhamnose + H2O
alpha-D-mannopyranose + rhamnose
-
part of the repeating oligosaccharide unit of the O-antigenic lipopolysaccharide in Salmonella typhimurium
-
?
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 alpha-D-mannopyranose
-
very low activity
-
?
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 alpha-D-mannopyranose
-
highly specific for alpha-1,2-mannosidic linkages
-
?
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 alpha-D-mannopyranose
-
-
-
?
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 D-mannopyranose
-
-
-
?
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 D-mannopyranose
-
-
-
?
3-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 alpha-D-mannopyranose
-
very low activity
-
?
3-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 alpha-D-mannopyranose
-
very low activity
-
?
6-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
alpha-D-mannopyranose
-
very low activity
-
?
6-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
alpha-D-mannopyranose
-
specific cleavage of alpha-1,4- or alpha-1,6-linkages
-
?
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O
6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc
-
-
-
?
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O
6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc
-
-
end products
?
glycopeptide + H2O
alpha-D-mannopyranose + ?
-
faster hydrolysis than for glycoprotein
-
?
glycopeptide + H2O
alpha-D-mannopyranose + ?
-
contains only alpha-1,2-linked mannose residues, highly specific on alpha-1,2-linked mannooligosaccharides
-
?
glycopeptide + H2O
alpha-D-mannopyranose + ?
-
from ovalbumin
-
?
glycopeptide + H2O
alpha-D-mannopyranose + ?
-
from ovalbumin
-
?
glycoprotein + H2O
alpha-D-mannopyranose + ?
-
ovomucoid, orosomucoid, ovalbumin
-
?
Man6GlcNAc + H2O
alpha-D-mannopyranose + ?
-
ordered hydrolysis of alpha(1-2)-residues followed by hydrolysis of alpha(1-3)- and alpha(1-6)-linked residues
-
?
Man9GlcNAc + 4 H2O
Man5GlcNAc + 4 alpha-D-mannopyranose
-
-
end product for Tris-eluted isoform, representing the Man5-oligosaccharide chain of the dolichol pathway formed in the cytosolic compartment during biosynthesis of N-glycosylprotein glycans
?
Man9GlcNAc + 4 H2O
Man5GlcNAc + 4 alpha-D-mannopyranose
-
hydrolyses alpha-1,2- and alpha-1,3-linkages
after 24 h incubation, specifically removes alpha-1,2-linked mannoses
?
Man9GlcNAc2 + H2O
Man5GlcNAc2 + Man6GlcNAc2 + Man7GlcNAc2 + Man8GlcNAc2 + alpha-D-mannopyranose
-
involved in N-glycan processing to form oligomannosidic glycans during glycoprotein biosynthesis, cleaves three of the four alpha-1,2-mannosidic linkages
major product: Man6GlcNAc2
?
Man9GlcNAc2 + H2O
Man5GlcNAc2 + Man6GlcNAc2 + Man7GlcNAc2 + Man8GlcNAc2 + alpha-D-mannopyranose
-
-
major product: Man5GlcNAc2, involved in Asn-linked oligosaccharide maturation
?
mannan + H2O
alpha-D-mannose + ?
-
from yeast
only mannose librated
?
mannan + H2O
alpha-D-mannose + ?
-
from baker's and sake yeast
-
?
mannan + H2O
alpha-D-mannose + ?
-
specific for alpha-1,2-linked mannopyranosyl residues
-
?
mannan + H2O
alpha-D-mannose + ?
-
derived from wild type or mutant strains of Saccharomyces cerevisiae, different specific activities for isoforms EI and EII, depending on the type of alpha-D-linkages of mutant substrates, preference for cleavage of alpha-1,6- and alpha-1,3-linkages
-
?
mannan + H2O
alpha-D-mannose + ?
-
yeast alpha-mannan, main product: alpha-D-mannose, hydrolysis of linear and branched mannans containing terminal alpha-1,2- and alpha-1,3-linked mannopyranosyl residues
-
?
mannopyranosyl-oligosaccharides + H2O
alpha-D-mannopyranose + ?
-
reverse action on alpha-D-mannopyranose, 85%, w/w, yields 0.93 M disaccharide with 4 different isomers, 0.61 M trisaccharide, 0.25 M tetrasaccharide and higher oligosaccharides, retention of anomeric configuration
-
r
mannopyranosyl-oligosaccharides + H2O
alpha-D-mannopyranose + ?
-
from yeast mannan, hydrolysis of alpha-1,2- and alpha-1,3-linkages
-
?
mannopyranosyl-oligosaccharides + H2O
alpha-D-mannopyranose + ?
-
from yeast mannan, hydrolysis of alpha-1,2- and alpha-1,3-linkages
-
?
mannopyranosyl-oligosaccharides + H2O
alpha-D-mannopyranose + ?
-
from yeast mannan, a tetrasaccharide terminating in a mannopyranosyl residue linked to C-3 of its neighbour is not hydrolysed
-
?
mannotetraose + H2O
alpha-D-mannopyranose + mannotriose
-
-
-
?
ribonuclease B + H2O
oligosaccharide + alpha-D-mannopyranose + ?
-
from bovine pancreas
single oligosaccharide component
?
TAKA-amylase A + H2O
alpha-D-mannopyranose + ?
-
from Aspergillus oryzae, hydrolysis of a single Man-1,2-linkage
-
?
additional information
?
-
-
the enzyme is involved in ordered degradation of glycoproteins, inborn enzyme-deficiency results in lysosomal storage disorder alpha-mannosidosis
-
-
?
additional information
?
-
Drosophila sp. (in: flies)
-
key enzyme in N-glycan processing
-
-
?
additional information
?
-
-
anomalies of the enzyme system detected in patients with amyotrophic lateral sclerosis could reflect a situation of progressive injury of proteins
-
-
?
additional information
?
-
-
the enzyme is required in the degradation of the asparagine-linked carbohydrates of glycoproteins. Deficiency of this enzyme leads to the lysosomal storage disorder alpha-mannosidosis
-
-
?
additional information
?
-
-
class 1 alpha1,2-mannosidases of glycosylhydrolase family 47 play critical roles in the maturation of Asn-linked glycoproteins in the endoplasmic reticulum and Golgi complex as well as in the recognition and timing of disposal of terminally unfolded proteins by ER-associated degradation, overview
-
-
?
additional information
?
-
the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein
-
-
?
additional information
?
-
-
the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein
-
-
?
additional information
?
-
the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein
-
-
?
additional information
?
-
higher eukaryotic GH38 alpha-mannosides play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer and autoimmune disease
-
-
?
additional information
?
-
-
the exo-type cytosolic class II enzyme cleaves off alpha-1,2-, alpha-1,3-, and alpha-1,6-mannose residues
-
-
?
