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3.2.1.22: alpha-galactosidase

This is an abbreviated version!
For detailed information about alpha-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.22

Reaction

alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
+
H2O
=
D-galactose
 +
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide

Synonyms

1,6-alpha-D-galactoside galactohydrolase, a-galactosidase, Ag-I, Ag-II, Aga-F78, Aga-Y, AgaA, agaAJB13, AgaB, AgaI, AGal, AgalB, Agalsidase alfa, AglA, AglC, AkalphaGal, alkaline alpha-gal form 1, alkaline alpha-galactosidase, alkaline alpha-galactosidase form 1, alpha-D-galactopyranoside galactohydrolase, alpha-D-galactosidase, Alpha-D-galactoside galactohydrolase, alpha-Gal, alpha-Gal A, alpha-Gal II, alpha-Gal III, alpha-gal1, alpha-galactosidase, alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3, alpha-galactosidase A, alpha-galactosidase AgaA A355E, alpha-galactosidase AgaB, alpha-galactosidase I, alpha-galactosidase II, alpha-galactosidase III, alpha-galactoside galactohydrolase, alphaGal1, ATSIP2, blAga3, BLGA_00330, ceramidase, galactosylgalactosylglucosyl-, ceramide trihexosidase, ceramidetrihexosidase, ceramidetrihexoside-alpha-galactosidase, E1 alpha-galactosidase, E2 alpha-galactosidase, E3 alpha-galactosidase, EC 3.2.1.47, Fabrazyme, Gal36, GALA, galA17, GalS, Genzyme, GH36 alpha-galactosidase, GH97b, GLA, LaMel36A, MEL1, Mel4A, MelA, melibiase, retaining alpha-galactosidase, ScAGal, Tm GalA, TM1192, TmGalA, TnGalA, trihexosyl ceramide galactosidase, trihexosylceramide alpha-galactosidase, trihexosylceramidealpha-galactosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.22 alpha-galactosidase

pH Stability

pH Stability on EC 3.2.1.22 - alpha-galactosidase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
at pH 10.0 the alpha-galactosidase activity is negligible
683002
2.2 - 8
-
-
714491
2.3
-
12 h, 62% of activity left
171264
3
-
30°C, 2 h, about 50% loss of activity
655258
3 - 10
-
activity is not detected when the enzyme is reacted in buffers under pH 3.0 or above pH 10.0, activity remains above 80% between pH 4.6-5.2, the enzyme retains more than 80% of its activity between pH 5.0 and 10.0
679586
3 - 12
-
695837
3 - 7.5
-
50°C, 5 h, stable
654803
3 - 8
3 - 9
-
2 h, 37°C
171193
3.5 - 8.5
alpha-Gal II is stable at pH 3.5-8.5 at 25°C
678818
3.6 - 9.9
-
-
714959
4 - 10
-
-
171266
4 - 11
the enzyme exhibits more than 40% of its initial activity after incubation in buffers ranging from pH 4.0 to 11.0 at 37°C for 1 h
730176
4 - 6
4 - 7
4 - 8
-
-
171255
4 - 9
-
remains stable in a wide pH range (4.0-9.0) at 55°C for 2 h
680117
4.1 - 6.7
more than 50% active between pH 4.1 and 6.7
680458
4.5 - 10
-
742095
4.5 - 6
Penicillium chrysogenum sp. 23
-
the enzyme activity remains stable over a wide range of pH 4.5-6.0 for 24 h
750215
4.5 - 9
-
697975
5 - 10
highly pH stable over the pH range 5.0-10.0
705088
5 - 7
-
stable
655258
5 - 8
-
75°C, 50 mM citrate-phosphate buffer, less than 40% loss of activity after 5 h
656702
5 - 9
5.5
-
4°C, 24 h, most stable at
655313
5.5 - 12
-
4°C, overnight, stable
655085
5.5 - 9
-
retains more than 90% of the initial activity at 45°C in a pH range from 5.5-9.0
678427
6 - 13
40°C, 1 h, stable
654253
6 - 8.5
-
-
171239
6.1
-
mutant enzyme S65T, more than 50% loss of activity after 20 min above
654330
6.4 - 8.3
-
55°C, stable for at least 24 h
741951
6.5
-
mutant enzyme S65A, more than 50% loss of activity after 20 min above
654330
6.9
-
mutant enzyme S66D, more than 50% loss of activity after 20 min above
654330
7 - 9
-
isoform Ag-I is highly active and stable over broad pH range showing more than 60% activity up to pH 9.0 with low activities at acidic pH. After 24 h of incubation at 36°C, isoform Ag-I retains 85% enzyme activity at pH 7.0, whereas isoform Ag-II retains 52% activity
749435
7.4
-
above no activity
136495
7.5 - 8
-
-
171239