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3.2.1.22: alpha-galactosidase

This is an abbreviated version!
For detailed information about alpha-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.22

Reaction

alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
+
H2O
=
D-galactose
+
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide

Synonyms

1,6-alpha-D-galactoside galactohydrolase, a-galactosidase, Ag-I, Ag-II, Aga-F78, Aga-Y, AgaA, agaAJB13, AgaB, AgaI, AGal, AgalB, Agalsidase alfa, AglA, AglC, AkalphaGal, alkaline alpha-gal form 1, alkaline alpha-galactosidase, alkaline alpha-galactosidase form 1, alpha-D-galactopyranoside galactohydrolase, alpha-D-galactosidase, Alpha-D-galactoside galactohydrolase, alpha-Gal, alpha-Gal A, alpha-Gal II, alpha-Gal III, alpha-gal1, alpha-galactosidase, alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3, alpha-galactosidase A, alpha-galactosidase AgaA A355E, alpha-galactosidase AgaB, alpha-galactosidase I, alpha-galactosidase II, alpha-galactosidase III, alpha-galactoside galactohydrolase, alphaGal1, ATSIP2, blAga3, BLGA_00330, ceramidase, galactosylgalactosylglucosyl-, ceramide trihexosidase, ceramidetrihexosidase, ceramidetrihexoside-alpha-galactosidase, E1 alpha-galactosidase, E2 alpha-galactosidase, E3 alpha-galactosidase, EC 3.2.1.47, Fabrazyme, Gal36, GALA, galA17, GalS, Genzyme, GH36 alpha-galactosidase, GH97b, GLA, LaMel36A, MEL1, Mel4A, MelA, melibiase, retaining alpha-galactosidase, ScAGal, Tm GalA, TM1192, TmGalA, TnGalA, trihexosyl ceramide galactosidase, trihexosylceramide alpha-galactosidase, trihexosylceramidealpha-galactosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.22 alpha-galactosidase

Temperature Stability

Temperature Stability on EC 3.2.1.22 - alpha-galactosidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
-
1 h, pH 7, 55% loss in activity
0 - 40
20
-
the enzyme is stable at 20°C for 24 h
25
-
1 h, pH 7, 85% loss in activity
25 - 55
the enzyme is active at moderately high temperatures (55°C), no activity loss is observed after 30 min of incubation at 50°C, at 25°C the enzyme still keeps 20% activity, whereas at temperatures above 55°C it drastically decreases
25 - 60
Penicillium chrysogenum sp. 23
-
the enzyme is stable from 25 to 40°C for 24 h. At 45°C the activity falls to 80%, at 50°C to 72% and at 60°C the activity is completely lost
30
-
up to 30°C the enzyme is stable
40 - 50
40 - 60
43
-
10 min, 50% loss in activity
45 - 95
-
the immobilized enzyme retains 100% of its activity when incubated at 45 and 65°C for 24 h. The half-lifes of the immobilized enzyme at 45, 65, 85 and 95°C are 4.4 h, 4.2 h, 3.9 h and 0.12 h, respectively. The enzyme has 75% of its activity after pre-incubation at 85°C for 30 h
47
-
10 min, 50% loss of activity, alpha-galactosidase 2
48
-
10 min, 50% loss of activity, alpha-galactosidase 3
50 - 60
the recombinant enzyme loses about 60% of the initial activity after incubation at 50°C for 30 min and is completely inactivated at 60°C for 5 min
52
-
1 h, 90% loss of activity
60 - 65
-
the enzyme is fairly stable at temperatures up to 60°C and has 87% of its full activity at 65°C after 2 h of incubation
60 - 70
-
loses 20% and 50% of the original activity in 30 min at 60°C and 70°C, respectively
62
-
5 min, 20% loss in activity
70 - 90
-
the half-life of isoform Ag-I varies from 1.85 h at 90°C to 7.6 h at 70°C
85
-
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 6 h
89.8
O33835
melting temperature of the wild type enzyme