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3.2.1.22: alpha-galactosidase

This is an abbreviated version!
For detailed information about alpha-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.22

Reaction

alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
+
H2O
=
D-galactose
 +
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide

Synonyms

1,6-alpha-D-galactoside galactohydrolase, a-galactosidase, Ag-I, Ag-II, Aga-F78, Aga-Y, AgaA, agaAJB13, AgaB, AgaI, AGal, AgalB, Agalsidase alfa, AglA, AglC, AkalphaGal, alkaline alpha-gal form 1, alkaline alpha-galactosidase, alkaline alpha-galactosidase form 1, alpha-D-galactopyranoside galactohydrolase, alpha-D-galactosidase, Alpha-D-galactoside galactohydrolase, alpha-Gal, alpha-Gal A, alpha-Gal II, alpha-Gal III, alpha-gal1, alpha-galactosidase, alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3, alpha-galactosidase A, alpha-galactosidase AgaA A355E, alpha-galactosidase AgaB, alpha-galactosidase I, alpha-galactosidase II, alpha-galactosidase III, alpha-galactoside galactohydrolase, alphaGal1, ATSIP2, blAga3, BLGA_00330, ceramidase, galactosylgalactosylglucosyl-, ceramide trihexosidase, ceramidetrihexosidase, ceramidetrihexoside-alpha-galactosidase, E1 alpha-galactosidase, E2 alpha-galactosidase, E3 alpha-galactosidase, EC 3.2.1.47, Fabrazyme, Gal36, GALA, galA17, GalS, Genzyme, GH36 alpha-galactosidase, GH97b, GLA, LaMel36A, MEL1, Mel4A, MelA, melibiase, retaining alpha-galactosidase, ScAGal, Tm GalA, TM1192, TmGalA, TnGalA, trihexosyl ceramide galactosidase, trihexosylceramide alpha-galactosidase, trihexosylceramidealpha-galactosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.22 alpha-galactosidase

Specific Activity

Specific Activity on EC 3.2.1.22 - alpha-galactosidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000833
-
value about, grown under low temperature for at least 3 days
0.00167
0.002083
-
value about, leaf, 8 days after anthesis
0.00375
-
value about, cold-sensitve cultivar, Guonong No. 41
0.004167
0.0067
-
value about, mesocarp tissue, 16 days after anthesis
0.0083
-
value about, mesocarp tissue, 12 days after anthesis
0.0125
-
value about, mesocarp tissue, day of anthesis and 8 days after anthesis
0.015
-
value about, mesocarp tissue, 2 days before anthesis
0.02
-
D-glucose
0.02083
-
value about, mesocarp tissue, 4 days after anthesis
0.0396
-
-
0.052
-
-
0.076
-
-
0.08
-
D-mannose
0.11
crude extract, using 4-nitrophenyl alpha-D-galactopyranoside as substrate, in 0.1 M Na+-phosphate buffer (pH 7.3), 0.15 M NaCl at 20°C
0.13
-
D-galactose
0.18
crude cell extract, in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C
0.26
-
melibiose
0.3
-
raffinose
0.31
alpha-Gal III, crude extract
0.41
-
guar gum
0.42
-
stachyose
0.46
Penicillium chrysogenum sp. 23
-
lyophilized extract, at pH 5.5 and 50°C
1.03
-
culture filtrate
1.2
-
crude filtrate
10.8
-
isoenzyme alpha-Gal III
104.9
-
recombinant enzyme
1080
-
isoenzyme alpha-Gal I
11.52
-
isoenzyme alpha-Gal IV
11275
-
after 1224.1fold purification, at pH 4.6 and 50°C
12
-
isoform Ag-I, after 7fold purification, at pH 7.0 and 36°C
1255
-
pH 5.0, 40°C, substrate 4-nitrophenyl-alpha-D-galactopyranoside
129
-
alpha-galactosidase I
137
substrate 4-nitrophenyl alpha-D-galactopyranoside, temperature not specified in the publication, pH 5
14.2
-
enzyme form P1
14.46
-
alpha-galactosidase 1
15.4
Halalkalibacterium halodurans
pH 7.4, 37°C, presence of NAD+, Mn2+, dithiothreitol
15.9
-
unpurified enzyme from fermentation broth, using p-nitrophenyl alpha-D-galactopyranoside as a substrate
1598
-
alpha-galactosidase I
1600
-
alpha-galactosidase I
1642
-
alpha-galactosidase II
17.6
-
-
198
substrate 4-nitrophenyl alpha-D-galactopyranoside, pH 4.5, 50°C
2.02
-
crude extract, in 0.1 M sodium acetate buffer, pH 5.0, at 40°C
2.1
-
alpha-galactosidase 3
2.25
alpha-Gal II, crude extract
2.3
-
alpha-galactosidase I
2.8
with cottonseed meal as substrate, at pH 5.0 and 60°C
2060000
-
placental alpha-galactosidase A
21.2
-
recombinant enzyme
21.95
-
isoenzyme alpha-Gal II
217
-
alpha-galactosidase II
221
with melibiose as substrate, at pH 5.0 and 60°C
224
-
activity with p-nitrophenyl alpha-D-galactopyranoside
229000
-
plasma alpha-galactosidase A
23.6
Penicillium duponti
-
-
230
-
larger form
231
after 2100fold purification, using 4-nitrophenyl alpha-D-galactopyranoside as substrate, in 0.1 M Na+-phosphate buffer (pH 7.3), 0.15 M NaCl at 20°C
28.3
-
alpha-galactosidase II
2800
-
alpha-galactosidase IV
285
-
alpha-galactosidase II
290
-
alpha-galactosidase I
295.6
-
-
3.03
-
lyophilized extract, at pH 5.0 and 40°C
3.33
-
crude extract, in 0.1 M sodium acetate buffer, pH 4.0, at 40°C
3.5
-
pH 5.0, 50°C, substrate galactomannan with galactose/mannose ratio 1:1.72
30.8
-
-
32
Coffea sp.
-
-
36.8
-
pH 5.0, 65°C
4.02
-
-
4.25
after 23fold purification, in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C
4.37
after 20.8fold purification
400
-
after 388fold purification
4070000
-
spleen alpha-galactosidase A
41
-
alpha-galactosidase II
452
-
alpha-galactosidase III
4600
-
alpha-galactosidase II
48.33
37°C
4900
-
alpha-galactosidase III
5.75
-
unpurified enzyme from culture broth
52.1
-
alpha-galactosidase II, p-nitrophenyl-alpha-D-galactopyranose as subtrate
52.8
alpha-Gal II, after 23.5fold purification
53.26
-
alpha-galactosidase 2
540
-
after 19.74fold purification, in 0.1 M sodium acetate buffer, pH 5.0, at 40°C
55
-
after 27.2fold purification, in 0.1 M sodium acetate buffer, pH 5.0, at 40°C
56.61
after 2461.3fold purification, using 4-nitrophenyl alpha-D-galactoside as substrate, at 37°C
6.2
-
pH 5.0, 50°C, substrate galactomannan with galactose/mannose ratio 1:2.4
6.56
-
-
6.9
with soybean meal as substrate, at pH 5.0 and 60°C
619
-
after 108fold purification
62.5
-
enzyme form P2
67.3
-
after 20.20fold purification, in 0.1 M sodium acetate buffer, pH 4.0, at 40°C
68.7
-
alpha-galactosidase I
74.1
Coffea sp.
-
-
74.6
-
after 60.4fold purification
74.62
-
purified recombinant enzyme
77.13
-
alpha-galactosidase II
8
-
isoform Ag-II, after 4.7fold purification, at pH 7.0 and 36°C
81.54
-
alpha-galactosidase I
82
-
alpha-galactosidase II
83.6
Tachigali multijuga
-
pH 5.0, 40°C
87.52
-
after 28.88fold purification, at pH 5.0 and 40°C
89150
-
-
9.13
alpha-Gal III, after 29.5fold purification
9.21
-
crude extract, at pH 4.6 and 50°C
94.5
Coffea sp.
-
-
98
-
pH 5.0, 40°C, substrate 4-nitrophenyl-alpha-D-galactopyranoside
additional information
-
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown, relative enzyme activities of whole fly homogenates low on substrates specific for alpha-galactosidase