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3.2.1.22: alpha-galactosidase

This is an abbreviated version!
For detailed information about alpha-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.22

Reaction

alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
+
H2O
=
D-galactose
 +
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide

Synonyms

1,6-alpha-D-galactoside galactohydrolase, a-galactosidase, Ag-I, Ag-II, Aga-F78, Aga-Y, AgaA, agaAJB13, AgaB, AgaI, AGal, AgalB, Agalsidase alfa, AglA, AglC, AkalphaGal, alkaline alpha-gal form 1, alkaline alpha-galactosidase, alkaline alpha-galactosidase form 1, alpha-D-galactopyranoside galactohydrolase, alpha-D-galactosidase, Alpha-D-galactoside galactohydrolase, alpha-Gal, alpha-Gal A, alpha-Gal II, alpha-Gal III, alpha-gal1, alpha-galactosidase, alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3, alpha-galactosidase A, alpha-galactosidase AgaA A355E, alpha-galactosidase AgaB, alpha-galactosidase I, alpha-galactosidase II, alpha-galactosidase III, alpha-galactoside galactohydrolase, alphaGal1, ATSIP2, blAga3, BLGA_00330, ceramidase, galactosylgalactosylglucosyl-, ceramide trihexosidase, ceramidetrihexosidase, ceramidetrihexoside-alpha-galactosidase, E1 alpha-galactosidase, E2 alpha-galactosidase, E3 alpha-galactosidase, EC 3.2.1.47, Fabrazyme, Gal36, GALA, galA17, GalS, Genzyme, GH36 alpha-galactosidase, GH97b, GLA, LaMel36A, MEL1, Mel4A, MelA, melibiase, retaining alpha-galactosidase, ScAGal, Tm GalA, TM1192, TmGalA, TnGalA, trihexosyl ceramide galactosidase, trihexosylceramide alpha-galactosidase, trihexosylceramidealpha-galactosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.22 alpha-galactosidase

Purification

Purification on EC 3.2.1.22 - alpha-galactosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
acetone precipitation and DEAE-Sepharose column chromatography
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affinity precipitation with alginate results in 40fold purification with 43% activity yield
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alpha-galactosidase 1
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alpha-galactosidase 2
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alpha-galactosidase 3
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ammonium sulfate fractionation, Sephacryl S-200 gel filtration
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ammonium sulfate fractionation, Sephadex G-100 gel filtration, CM-Sepharose Fast Flow column chromatography
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ammonium sulfate fractionation, Sepharose CL-6B column chromatography, DEAE-Sepharose FF column chromatography, and Superose 12 gel filtration
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ammonium sulfate precipitation
ammonium sulfate precipitation, DEAE Toyopearl column chromatography, Sepharose CL-4B column chromatography, and Source 15Q PE gel filtration
ammonium sulfate precipitation, HiTrap Q Sepharose XL column chromatography, and Sephacryl S-200 HR gel filtration
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ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl S-300 gel filtration
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anti-Flag M2 affinity resin column chromatography
DEAE column chromatography and Fractogel SO3- gel filtration
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DEAE-cellulose column chromatography and Sephadex G-100 gel filtration
Penicillium chrysogenum sp. 23
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DEAE-cellulose column chromatography, Q Sepharose column chromatography, Mono Q column chromatography, and Superdex G-75 gel filtration
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DMAE column chromatography, Mono Q column chromatography and Superdex 200 gel filtration
efficient and rapid purification procedure for recombinant alpha-galactosidase A by sequential Concanavalin A-Sepharose and immobilized thio-alpha-galactoside agarose column chromatography. This procedure is especially useful for the purification of mutant forms of alpha-galactosidase A, which are not stable under conventional purification techniques. Purification of the intracellular mutant enzyme M279I, expressed in COS-7 cells within 6 h at 62% overall yield
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from germination seed
Tachigali multijuga
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isoenzymes AgaS-m1, AgaS-m2, AgaS-m3 isolated from media with galactomannan and isoenzymes AgaS-b1, AgaS-b2, AgaS-b3 from media with wheat bran
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multiple isoforms: alpha-Gal I, alpha-Gal II, alpha-Gal III, alpha-Gal IV
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Ni-chelating Sepharose column chromatography and DEAE-Sepharose column chromatography
Ni-NTA agarose column chromatography
Ni-NTA agarose column chromatography and Superdex 200 gel filtration
Ni-NTA column chromatography
Ni2+-Sepharose column chromatography
one of the enzyme preparations, P1, shows a single protein with 33000 Da and the second preparation, P2, has two proteins with 31000 Da and 33000 Da
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partial purification by DEAE-Sepharose column chromatography
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phenyl Sepharose CL-4B column chromatography
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production of alpha-galactosidase by Humicola lutea immobilized in a hybrid sol-gel matrix consisting of tetraethylorthosilicate as a precursor and a mixture of polyethyleneglycol and polyvinylalcohol. Best results were obtained with 2 g of the sol-gel particles per culture flask using 144-h runs, giving a high level of enzyme titer exceeding the activity of free cells during four cycles of operation
-
Q Sepharose Fast Flow column chromatography and ammonium sulfate precipitation
Q-Sepharose Fast Flow column chromatography, hydroxyapatite column chromatography, Mono Q 5/50 GL column chromatography, and Superdex 200 HR 10/30 gel filtration
recombinant alpha-galactosidase AgaA A355E
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recombinant alpha-galactosidase AgaB
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recombinant enzyme
-
separation and purification technique for both alpha-galactosidase and invertase simply using ammoniumsulphate and tert-butanol under different conditions. alpha-Galactosidase and invertase are purified 15- and 12-fold with 50 and 54% activity recovery, respectively. Method is non-chromatographic and economical for protein purification
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Sephacryl S-200 gel filtration
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Talon metal affinity resin column chromatography, and Superdex 200 gel filtration
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three-phase partitioning method. The addition of n-butanol, t-butanol, and isopropanol in the presence of ammonium sulfate pushes the protein out of the solution to form an interfacial precipitate layer between the lower aqueous and upper organic layers, resulting in 12fold purification with activity recovery of 92%
-
using Ni-NTA chromatography