3.2.1.22: alpha-galactosidase
This is an abbreviated version!
For detailed information about alpha-galactosidase, go to the full flat file.
Word Map on EC 3.2.1.22
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3.2.1.22
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fabry
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lysosomal
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x-linked
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glycosphingolipids
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cardiac
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globotriaosylceramide
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endothelial
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galactose
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epitope
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oligosaccharide
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pain
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raffinose
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hypertrophy
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ventricular
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proteinuria
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glycosidases
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glycolipids
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angiokeratomas
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alpha-glucosidase
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melibiose
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cerebrovascular
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galactomannans
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simplicifolia
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multisystemic
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corporation
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xenotransplantation
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anaphylaxis
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hemizygotes
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cambridge
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alpha-l-fucosidase
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stachyose
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coffee
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hyperacute
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anti-gal
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beta-d-glucuronidase
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analysis
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bandeiraea
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alpha-n-acetylgalactosaminidase
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guar
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beta-d-galactosidase
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biotechnology
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sanofi
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nutrition
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griffonia
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beta-mannosidase
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imiglucerase
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degradation
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gaucher
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molecular biology
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beta-d-glucosidase
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medicine
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synthesis
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verticillata
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soymilk
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alpha-d-mannosidase
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arylamidase
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flatulence
- 3.2.1.22
- fabry
- lysosomal
-
x-linked
- glycosphingolipids
- cardiac
- globotriaosylceramide
- endothelial
- galactose
- epitope
- oligosaccharide
- pain
- raffinose
- hypertrophy
- ventricular
- proteinuria
- glycosidases
- glycolipids
- angiokeratomas
- alpha-glucosidase
- melibiose
- cerebrovascular
- galactomannans
- simplicifolia
-
multisystemic
-
corporation
-
xenotransplantation
- anaphylaxis
-
hemizygotes
-
cambridge
- alpha-l-fucosidase
- stachyose
- coffee
-
hyperacute
-
anti-gal
- beta-d-glucuronidase
- analysis
-
bandeiraea
- alpha-n-acetylgalactosaminidase
- guar
- beta-d-galactosidase
- biotechnology
-
sanofi
- nutrition
- griffonia
- beta-mannosidase
- imiglucerase
- degradation
- gaucher
- molecular biology
- beta-d-glucosidase
- medicine
- synthesis
-
verticillata
-
soymilk
- alpha-d-mannosidase
- arylamidase
-
flatulence
Reaction
Synonyms
1,6-alpha-D-galactoside galactohydrolase, a-galactosidase, Ag-I, Ag-II, Aga-F78, Aga-Y, AgaA, agaAJB13, AgaB, AgaI, AGal, AgalB, Agalsidase alfa, AglA, AglC, AkalphaGal, alkaline alpha-gal form 1, alkaline alpha-galactosidase, alkaline alpha-galactosidase form 1, alpha-D-galactopyranoside galactohydrolase, alpha-D-galactosidase, Alpha-D-galactoside galactohydrolase, alpha-Gal, alpha-Gal A, alpha-Gal II, alpha-Gal III, alpha-gal1, alpha-galactosidase, alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3, alpha-galactosidase A, alpha-galactosidase AgaA A355E, alpha-galactosidase AgaB, alpha-galactosidase I, alpha-galactosidase II, alpha-galactosidase III, alpha-galactoside galactohydrolase, alphaGal1, ATSIP2, blAga3, BLGA_00330, ceramidase, galactosylgalactosylglucosyl-, ceramide trihexosidase, ceramidetrihexosidase, ceramidetrihexoside-alpha-galactosidase, E1 alpha-galactosidase, E2 alpha-galactosidase, E3 alpha-galactosidase, EC 3.2.1.47, Fabrazyme, Gal36, GALA, galA17, GalS, Genzyme, GH36 alpha-galactosidase, GH97b, GLA, LaMel36A, MEL1, Mel4A, MelA, melibiase, retaining alpha-galactosidase, ScAGal, Tm GalA, TM1192, TmGalA, TnGalA, trihexosyl ceramide galactosidase, trihexosylceramide alpha-galactosidase, trihexosylceramidealpha-galactosidase
ECTree
3.2.1.22 alpha-galactosidaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.2.1.22 - alpha-galactosidase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.22
1-naphthyl alpha-D-galactoside
temperature not specified in the publication, pH 5
0.03
alpha-D-galactosyl fluoride
temperature not specified in the publication, pH 5
37.4
p-nitrophenyl-beta-L-arabinopyranoside
in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C
0.037
2,4-dinitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.135
2,4-dinitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.143
2,5-dinitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.243
2,5-dinitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.3
2-nitrophenyl alpha-D-galactopyranoside
temperature not specified in the publication, pH 5
0.133
2-nitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.143
2-nitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.086
3,4-dimethylphenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.089
3,4-dimethylphenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.096
3-nitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.14
3-nitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.104
4-bromophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.111
4-bromophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
1.8
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme Q279E, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
1.9
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme R301Q, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
2
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme R356W, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
2.4
4-methylumbelliferyl-alpha-D-galactoside
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mutant enzyme M296V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
2.6
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme L166V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
2.7
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme M296I, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
2.8
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme A20P, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
2.8
4-methylumbelliferyl-alpha-D-galactoside
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mutant enzyme N215S, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
2.8
4-methylumbelliferyl-alpha-D-galactoside
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wild type enzyme, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
3.1
4-methylumbelliferyl-alpha-D-galactoside
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mutant enzyme E66Q, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
3.2
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme M72V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
3.4
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme A156V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
3.4
4-methylumbelliferyl-alpha-D-galactoside
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mutant enzyme I91T, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
3.6
4-methylumbelliferyl-alpha-D-galactoside
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mutant enzyme F113L, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
3.7
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme A97V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
5.8
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme R112H, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
16.3
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme E59K, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C
0.04
4-nitrophenyl alpha-D-galactopyranoside
pH 5.0, 65°C, wild-type enzyme
0.1
4-nitrophenyl alpha-D-galactopyranoside
37°C, pH 5.0, mutant enzyme 328A
0.1
4-nitrophenyl alpha-D-galactopyranoside
37°C, pH 5.0, mutant enzyme F194K
0.1
4-nitrophenyl alpha-D-galactopyranoside
pH 5.0, 65°C, mutant enzyme D327G
0.11
4-nitrophenyl alpha-D-galactopyranoside
37°C, pH 5.0, wild-type enzyme
0.11
4-nitrophenyl alpha-D-galactopyranoside
37°C, pH 5.0, mutant enzyme G385L
0.11
4-nitrophenyl alpha-D-galactopyranoside
37°C, pH 5.0, mutant enzyme P402S
0.12
4-nitrophenyl alpha-D-galactopyranoside
pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3
0.2
4-nitrophenyl alpha-D-galactopyranoside
temperature not specified in the publication, pH 5
0.21
4-nitrophenyl alpha-D-galactopyranoside
wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
0.24
4-nitrophenyl alpha-D-galactopyranoside
mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
0.32
4-nitrophenyl alpha-D-galactopyranoside
37°C, pH 5.0, mutant enzyme L195C
0.43
4-nitrophenyl alpha-D-galactopyranoside
37°C, pH 5.0, mutant enzyme P402D
0.44
4-nitrophenyl alpha-D-galactopyranoside
mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
0.47
4-nitrophenyl alpha-D-galactopyranoside
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immobilized enzyme, at pH 5.0 and 40°C
0.83
4-nitrophenyl alpha-D-galactopyranoside
wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
0.84
4-nitrophenyl alpha-D-galactopyranoside
mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
1.13
4-nitrophenyl alpha-D-galactopyranoside
-
pH not specified in the publication, temperature not specified in the publication
1.31
4-nitrophenyl alpha-D-galactopyranoside
pH 5.0, 37°C
1.33
4-nitrophenyl alpha-D-galactopyranoside
mutant enzyme W336D of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
8.7
4-nitrophenyl alpha-D-galactopyranoside
mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
0.08
4-nitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.083
4-nitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.19
4-nitrophenyl alpha-D-galactoside
mutant enzyme D415N, in 40 mM sodium phosphate buffer, pH 6.0, at 35°C
0.31
4-nitrophenyl alpha-D-galactoside
wild type enzyme, in 40 mM sodium phosphate buffer, pH 6.0, at 35°C
0.32
4-nitrophenyl alpha-D-galactoside
mutant enzyme E470Q, in 40 mM sodium phosphate buffer, pH 6.0, at 35°C
0.24
4-nitrophenyl-alpha-D-galactopyranoside
mutant Q251W, pH 7.4, 30°C
1.4
4-nitrophenyl-alpha-D-galactopyranoside
mutant Q251A, pH 7.4, 30°C
3.7
4-nitrophenyl-alpha-D-galactopyranoside
mutant A41Y, pH 7.4, 30°C
4.5
4-nitrophenyl-alpha-D-galactopyranoside
wild-type, pH 7.4, 30°C
0.048
4-O-methylphenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.088
4-O-methylphenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.16
6-bromo-2-naphthyl alpha-D-galactoside
temperature not specified in the publication, pH 5
0.039
beta-naphthyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.225
beta-naphthyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.085
p-nitrophenyl alpha-D-galactopyranoside
in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C
0.22
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50°C, isoenzyme alpha-Gal II
0.24
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50°C, isoenzyme alpha-Gal IV
0.27
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50°C, isoenzyme alpha-Gal III
1.13
p-nitrophenyl alpha-D-galactopyranoside
-
at 55°C, in 50 mM citrate buffer (pH 4.2)
1.4
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50°C, isoenzyme alpha-Gal I
2.9
p-nitrophenyl alpha-D-galactopyranoside
-
in 0.1 M McIlvaine buffer (pH 4.8) at 37°C
0.23
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase II, pH 6
0.47
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase I, pH 7
0.53
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase II, pH 7
0.59
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase I, pH 6
0.176
phenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
0.324
phenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C
1.61
raffinose
-
pH not specified in the publication, temperature not specified in the publication
3.8
raffinose
wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
36.6
raffinose
mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
200
raffinose
wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
205
raffinose
mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
250
raffinose
mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
252
raffinose
mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
1.17
stachyose
-
pH not specified in the publication, temperature not specified in the publication
additional information
additional information
hydrolysis of alpha-galactosides with poor leaving groups is Michaelian, but that of substrates with good leaving groups exhibits pronounced apparent substrate inhibition, with Kis values similar to Km values
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additional information
additional information
-
hydrolysis of alpha-galactosides with poor leaving groups is Michaelian, but that of substrates with good leaving groups exhibits pronounced apparent substrate inhibition, with Kis values similar to Km values
-
