Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.22: alpha-galactosidase

This is an abbreviated version!
For detailed information about alpha-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.22

Reaction

alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
+
H2O
=
D-galactose
 +
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide

Synonyms

1,6-alpha-D-galactoside galactohydrolase, a-galactosidase, Ag-I, Ag-II, Aga-F78, Aga-Y, AgaA, agaAJB13, AgaB, AgaI, AGal, AgalB, Agalsidase alfa, AglA, AglC, AkalphaGal, alkaline alpha-gal form 1, alkaline alpha-galactosidase, alkaline alpha-galactosidase form 1, alpha-D-galactopyranoside galactohydrolase, alpha-D-galactosidase, Alpha-D-galactoside galactohydrolase, alpha-Gal, alpha-Gal A, alpha-Gal II, alpha-Gal III, alpha-gal1, alpha-galactosidase, alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3, alpha-galactosidase A, alpha-galactosidase AgaA A355E, alpha-galactosidase AgaB, alpha-galactosidase I, alpha-galactosidase II, alpha-galactosidase III, alpha-galactoside galactohydrolase, alphaGal1, ATSIP2, blAga3, BLGA_00330, ceramidase, galactosylgalactosylglucosyl-, ceramide trihexosidase, ceramidetrihexosidase, ceramidetrihexoside-alpha-galactosidase, E1 alpha-galactosidase, E2 alpha-galactosidase, E3 alpha-galactosidase, EC 3.2.1.47, Fabrazyme, Gal36, GALA, galA17, GalS, Genzyme, GH36 alpha-galactosidase, GH97b, GLA, LaMel36A, MEL1, Mel4A, MelA, melibiase, retaining alpha-galactosidase, ScAGal, Tm GalA, TM1192, TmGalA, TnGalA, trihexosyl ceramide galactosidase, trihexosylceramide alpha-galactosidase, trihexosylceramidealpha-galactosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.22 alpha-galactosidase

Inhibitors

Inhibitors on EC 3.2.1.22 - alpha-galactosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S,3R,4R,5S)-2-(hydroxymethyl)-5-[(methylamino)methyl]pyrrolidine-3,4-diol
-
-
(2S,3R,4R,5S)-2-[(ethylamino)methyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol
-
-
1-deoxy-L-altronojirimycin hydrochloride
1000fold less effective than 1-deoxygalactonojirimycin
1-deoxygalactonojirimycin
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
31% residual activity at 100 mM, at pH 6.0
1-ethyl-3-(dimethylaminopropyl) carbodiimide
2,4,6-Trinitrobenzenesulfonic acid
-
20% residual activity at 2 mM, at pH 8.4
2-deoxy-D-galactose
-
effective inhibitor of alpha-galactosidase
2-mercaptoethanol
AgNO3
-
1.0 mM, complete inhibition, alpha-galactosidase 1; 1.0 mM, complete inhibition, alpha-galactosidase 2; 1.0 mM, complete inhibition, alpha-galactosidase 3
alpha-D-galactopyranosyl azide
-
75% inhibition at 2 mM
alpha-D-galactose
-
2 mM, 17% inhibition of enzyme P1, 68.5% inhibition of enzyme P2
beta-D-galactose
competitive
CaCl2
-
10 mM, 19.9% inhibition
CdSO4
-
0.25 mM, 99.9% inhibition
Ceramide lactoside
-
-
ceramide trihexoside
citraconic anhydride
-
30% residual activity at 5 mM, at pH 7.8
CoSO4
-
0.25 mM, 8.3% inhibition
CuCl2
CuSO4
-
2 mM, 94.5% inhibition of enzyme P1, 48% inhibition of enzyme P2
D-fucose
D-galactono-1,4-lactone
-
inhibits beta-galactosidase more effectively than alpha-galactosidase
D-galactose
D-glucose
D-lactose
D-maltose
D-mannose
D-myo-inositol
-
weak competitive inhibitor
D-sucrose
-
98.72% activity at 10 mM
D-xylose
diethyldicarbonate
-
complete inhibition at 2 mM, at pH 6.0
digalactosylceramide
dithiothreitol
-
54% residual activity at 0.4 mM
FeCl2
-
1 mM, 50% inhibition
FeCl3
-
1.0 mM, 46.2% inhibition
FeSO4
fructose
galactose
galactostatin bisulfite
gamma-1,4-galactonolactone
glucosylsphingosine
-
psychosine
glycerol 3-phosphate
-
-
HgCl2
Human serum albumin
-
-
-
iodacetamide
iodoacetamide
iodoacetic acid
-
-
KCl
-
2.0 M, 41.3% inhibition; 2.0 M, 74.6% inhibition, alpha-galactosidase 3; 2.0 M, 8.8% inhibition, alpha-galactosidase 1
L-arabinose
L-ascorbic acid
-
-
lactose
mannose
-
70% inhibition of alpha-Gal I at 40 mM
melibiose
methyl alpha-D-galactopyranoside
-
15% inhibition at 2 mM
methyl beta-D-galactopyranoside
-
13% inhibition at 2 mM
MgCl2
-
10 mM, 16.4% inhibition
MnCl2
-
1.0 mM, 18.4% inhibition
myo-inositol
N-(N-benzyloxycarbonyl-epsilon-aminocaproyl)-alpha-D-galactopyranoside
Coffea sp.
-
-
N-acetyl-D-galactosamine
-
-
N-bromosuccinimide
n-butyldeoxygalactonojirimycin
2000fold less effective than 1-deoxygalactonojirimycin
N-epsilon-Aminocaproyl-alpha-D-galactopyranoside
Coffea sp.
-
-
N-ethyl maleimide
NEM
-
10 mM, 12.9% inhibition
Ni(2+)
-
92.7% activity at 1 mM
NiCl2
-
1.0 mM, 21.7% inhibition
p-chloromercuribenzoate
p-hydroxymercuribenzoate
-
0.25 mM, 97.8% inhibition
p-nitrophenyl-alpha-D-galactoside
-
substrate inhibition above 4 mM, alpha-galactosidase 2; substrate inhibition above 4 mM, alpha-galactosidase 3
paraoxon
-
-
raffinose
Sodium dodecyl sulfate
sodium taurocholate
-
-
stachyose
sucrose
Urea
-
isoform Ag-II shows 92% residual activity at 5 mM urea
Woodward's reagent K
-
21% residual activity at 5 mM, at pH 6.0
ZnCl2
-
1.0 mM, 99.3% inhibition
additional information
-