3.2.1.21: beta-glucosidase This is an abbreviated version! For detailed information about beta-glucosidase, go to the full flat file .
Reaction
celloheptaose +
6 H2O
=
7 beta-D-glucose
Synonyms 1,4-beta-glucosidase, 3BGlu6, 4BGlu12, A1, amygdalase, amygdalin hydrolase, amygdalinase, arbutinase, aryl beta-glucosidase, aryl-beta-D-glucosidase, aryl-beta-glucosidase, AT5A_15831, beta-1,6-glucosidase, beta-D-glucosidase, beta-D-glucosidase F1, beta-D-glucoside glucohydrolase, beta-Glcase, beta-glu, beta-Glu x, beta-glu1, beta-glu2, beta-glucosidase, beta-glucosidase 1, beta-glucosidase 6, beta-glucosidase B, beta-glucosidase C, beta-glucosidase I, beta-glucosidase II, beta-glucoside hydrolase, beta-GLYPI, betaG, betagly, BG, BG1, BGA, Bgl, BGL1, BGL1A, BGL1B, Bgl2, Bgl3, bgl3a, Bgl3B, BGL50, BglA, BglB, BglC, BglD5, Bglhi, BglHi2, BglI, BglII, BglP, BGLPf, BglT, BGLU, BGlu1, BGLU21, BGLU22, BGLU23, Bglu3B, bgpA, BGs, BGX1, bifunctional beta-glucosidase/xylosidase, bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase, Cba3, CBG, CC1G_08724, Cel3A, Cel3b, CelB, cellobiase, CGHII, cyanogenic beta-glucosidase, dalcochinase, Dhr1, dhurrinase-1, DV-BG, elaterase, emulsin, esculinase, F1, family-1 beta-glycosidase, FPG, G-II, GBA, GBA2, GBA3, gentiobiase, GH1 beta-glucosidase, GH3 beta-glucosidase, Gh3-4, GI, GII, ginsenoside-hydrolyzing beta-D-glucosidase, Gl-2, Gl-3, Glu1, Glu1b, Glu2, GLU3, GLU4, hCBG, HGT-BG, HiBgl3A, HiBgl3B, HiBgl3C, Hore_15280, ICHG, iridoid beta-glucoside, isoflavone conjugate-hydrolyzing beta-glucosidase, J1, Klotho-related protein, KLrP, KNOUC202beta-gly, lactase, limarase, Lin1840, linamarase, linustatinase, LjBGD2, LjBGD4, LjBGD7, MeBglD2, More, Novozyme 188, oligofurostanoside-specific beta-glucosidase, OsTAGG, p-nitrophenyl beta-glucosidase, PF0073, PGI, PGII, piceid-beta-D-glucosidase, PRGH1, primeverosidase, prunasin hydrolase, reCBG, RuBGX1, SA-bglu, salicilinase, salicinase, SbDhr1, Sde1394, SP188, SSO3039, SYNbglA, T-cell inhibitor, T1, Td2F2, Tnap_0656, Tpbgl, TpBGL1, TpBGL3, Tpebgl3, Tpen_1494, tuberonic acid glucoside (TAG)-hydrolyzing beta-glucosidase, tuberonic acid glucoside beta-glucosidase, Umbgl3B, vicianase, ZM-p60.1, ZmGlu1
ECTree
pH Stability
pH Stability on EC 3.2.1.21 - beta-glucosidase
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10.3
-
inactivation
664628
2
-
purified enzyme, 25°C, 24 h, over 20% activity remaining
731421
2 - 10
-
stable within for 30 min
326303
2 - 8
-
stable within
136306
2.5 - 10
-
stable within for 24 h at 4°C
326361
2.5 - 6
-
stable within, beta-glucosidase 3
326318
2.5 - 7
the activity drops by 50% at pH 2.5 and 6.0, and is negligible from pH 7.0 upward
706319
2.5 - 9
-
no loss of activity in 2 h at 4°C
326316
3 - 11
-
24 h, completely stable
744079
3 - 5
-
approx. 80% loss of activity after 1 h at pH 3.0 and 28°C
655757
3 - 6
-
purified enzyme, 24 h, over 80% activity remaining
731421
3.15
-
approx. 80% loss of activity after 24 h
655767
3.3 - 10.3
-
fairly stable, 90% of the original activity retains after incubation at 4°C for 16 h
710240
3.5
42% of the initial activity for the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde remains, the activity of free enzyme is completely lost
746352
3.5 - 4
90°C, 1 or 2 h, more than 80% of the maximum activity is retained
728007
3.5 - 8.5
-
approx. 30% loss of activity after 2 h at pH 3.5 and 30°C, approx. 20% loss of activity after 2 h at pH 8.5 and 30°C
655773
3.5 - 9.5
-
no loss of activity after 20 min at 25°C
655724
3.6 - 8
-
beta-glucosidase II
326301
4 - 5
-
30 min, purified piceid-beta-D-glucosidase, stable
682614
4 - 5.5
-
approx. 50% loss of activity after 24 h
655767
4.5 - 6.5
-
purified enzyme, stable
682609
4.5 - 7
-
stable for 2 h at 70°C
326325
4.5 - 9.5
-
purified enzyme, stable
682609
5
-
stable when the pH of the storage buffer is above 5.0
742132
5 - 8.5
mutated recombinant beta-glucosidase is fairly stable at pH 5.0 and 8.5, exhibiting 60 and 62% activities, respectively. It retains over 50% of the original activity between pH 4-7.5. Between pH values 6.0 and 7.2, it retains over 98% activity after incubation at 37°C for 2 h
696166
5.2
-
stable for 48 h
741847
5.5 - 7.5
-
for PGII
326271
5.5 - 8.5
-
no loss of activity
655652
5.5 - 9.5
-
fairly stable
664149
5.6 - 8
stable, purified recombinant enzyme
664418
6
beta-glucosidase TpBGL1 shows higher stability at pH 6 than at pH 4
746720
6 - 12
-
complete loss of activity below pH 6.0 after 4 h at 20°C, 37% loss of activity after 4 h at pH 12.0 and 20°C
655770
6 - 7
recombinant enzyme, stable
664656
6.5
-
stable for 2 h at 15-40°C
326340
3 - 10
-
the enzyme retains about 60% activity at pH 3-6 and only 20% activity at pH 10
763210
3 - 10
about 60% activity at pH 3.0, about 80% activity at pH 4.0, the enzyme is stable at pH 5.0-9.0, activity drops sharply at pH 10.0
702747
3 - 7
-
stable within, beta-glucosidase 2
326318
3 - 7
-
purified enzyme, 4 h, 45°C, stable
678825
3 - 7
no activity at pH 3.0 and 7.0, about 20% activity at pH 4.0, about 40% activity at pH 6.0
701871
3 - 7
24 h, almost 90% residual activity
743400
3 - 8
-
stable within
326310
3 - 8
-
increase of enzyme activity with pH increase, above 8 decrease of enzyme activity with increasing pH
702988
3 - 8
-
no loss of activity
654214
3 - 8
purified His-tagged recombinant enzyme, quite stable
677741
3 - 9
-
40% loss of activity after 24 h at 0°C and pH 3.0, approx. 20% loss of activity after 24 h at 0°C and pH 4.0-9.0
654873
3 - 9
-
40% loss of activity after 24 h at 0°C and pH 3.0, approx. 205 loss of activity after 24 h at 0°C and pH 4.0-9.0
654873
3 - 9
-
the enzyme is stable for 24 h at pH 3.0 to 9.0
750144
3.5 - 7
-
stable within, beta-glucosidase 1
326318
4
-
incubation at pH 4.0 leads to 43.3-44.2% residual activity compared to the activity at pH 6.0
701455
4
-
unstable below
696894
4
-
incubation at pH 4.0 leads to 79.4% residual activity compared to the activity at pH 6.0
701455
4
-
incubation at pH 4.0 leads to 62.4% residual activity compared to the activity at pH 6.0
701455
4
-
incubation at pH 4.0 leads to 98.4% residual activity compared to the activity at pH 6.0
701455
4
-
incubation at pH 4.0 leads to 27% residual activity compared to the activity at pH 6.0
701455
4
-
incubation at pH 4.0 leads to 119.6% residual activity compared to the activity at pH 6.0
701455
4
-
incubation at pH 4.0 leads to 79.7-89.6% residual activity compared to the activity at pH 6.0
701455
4
-
incubation at pH 4.0 leads to 88.5% residual activity compared to the activity at pH 6.0
701455
4
-
incubation at pH 4.0 leads to 45.1% residual activity compared to the activity at pH 6.0
701455
4 - 10
-
30°C, stable for 24 h
679559
4 - 10
-
stable between pH 4.0 and 10.0 at 4°C
702775
4 - 4.5
-
stable within
326337
4 - 4.5
-
most stable within
326360
4 - 6
-
about 80% of the relative activity at 45°C is retained at both pH 4.0 and 5.0. 5% activity at pH 3.5
715222
4 - 6
stable in the range of pH 4.0 to 6.0 while outside this pH range no residual activity is measured, at 65°C the residual activity is 50% at pH 5.0 and 6.0 (after 24 h) and 20% at pH 4.0
701870
4 - 6
-
stable within, beta-glucosidase I
326301
4 - 6.5
-
the enzyme activity remains more than 70% after incubating at pH 4.0-6.5 at 4°C for 24 h
705193
4 - 6.5
-
50°C, stable for at least 6 h
743015
4 - 7
-
3 h, more than 60% residual activity
744278
4 - 7
-
the free enzyme is stable at pH 7.0 (half-life of 50 days) but not at pH 4.0 (half-life of 4 days)
704235
4 - 8
-
stable within
326290
4 - 8
-
stable within
326320
4 - 8
native and recombinant enzyme are stable over a broad pH range of 4.0-8.0 with maximum retention of their catalytic activity. However, activities decline quickly below pH 4.0
751822
4 - 8
-
stable within
326320
4 - 9
-
chimeric enzymes, stable within
326290
4 - 9
-
chimeric enzymes, stable within
326290
4 - 9
stable for 30 min
744936
4 - 9
-
no loss of activity
655652
4.5 - 6
90°C, 1 or 2 h, 60% of the activity is retained
728007
4.5 - 6
the enzyme is unstable below pH 6.0, at pH 6.0 its half-life at 75°C is 28 days but at pH 4.5 is 13 h
702822
4.5 - 6
-
stable within, soluble and immobilized enzyme preparations
326305
4.5 - 8
-
fairly stable
663462
5 - 10
-
stable within
326290
5 - 10
37°C, 1 h, enzyme retains more than 80% activity
739692
5 - 6
Melanocarpus sp.
-
purified enzyme, stable
679482
5 - 6
-
the enzyme is stable from pH 5.0-6.0 for 120 min at 37°C
704983
5 - 7
-
-
326358
5 - 7
-
stable within, kept at 4°C
136321
5 - 7.5
-
723386
5 - 7.5
-
purified enzyme, most stable at pH 7.5, inactivation below pH 5.0
678824
5 - 8
-
25°C, stable up to 1 h
742537
5 - 8
37°C, 1 h incubation, the enzyme retains more than 80% of its maximum activity
724664
5 - 9
-
rapid inactivation below and above for cellulose-immobilized Cex-cellulose-binding domain fused to beta-glucosidase
326302
5 - 9
37°C, 1 h, enzyme retains more than 80% activity
739692
5 - 9
-
residual activity is 80-100% between pH ranges 5.0-8.0, the stability of the enzyme in mycelia extract is comparatively low at acidic pH and the enzyme is most stable at pH 9.0, the purified enzyme shows more than 95% residual activity between pH ranges 6.0-7.0
702717
6 - 7.5
-
-
326358
6 - 7.5
-
retaining more than 80% of the activity
326272
6 - 8
-
-
744539
6 - 8
-
yeast foam strain
326358
6 - 8
-
stable within
326319
6 - 9
-
25°C, stable for 24 h
738769
6 - 9
-
no loss of activity after 1 h at 28°C
655757
7
-
purified enzyme, 25°C, 24 h, over 50% activity remaining
731421
7
-
approx. 40% loss of activity after 24 h
655767
8
and above, 2 h, no loss of activity
701071
8
2 h, no loss of activity
746384
8
-
purified enzyme, 25°C, 24 h, over 20% activity remaining
731421
8
-
rapid decrease of activity above
326275
8
the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde retains 79.6% of the initial activity underalkaline conditions (pH 8.0), which is higher by 32% compared with native enzyme
746352