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3.2.1.21: beta-glucosidase

This is an abbreviated version!
For detailed information about beta-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.21

Reaction

celloheptaose
+ 6 H2O = 7 beta-D-glucose

Synonyms

1,4-beta-glucosidase, 3BGlu6, 4BGlu12, A1, amygdalase, amygdalin hydrolase, amygdalinase, arbutinase, aryl beta-glucosidase, aryl-beta-D-glucosidase, aryl-beta-glucosidase, AT5A_15831, beta-1,6-glucosidase, beta-D-glucosidase, beta-D-glucosidase F1, beta-D-glucoside glucohydrolase, beta-Glcase, beta-glu, beta-Glu x, beta-glu1, beta-glu2, beta-glucosidase, beta-glucosidase 1, beta-glucosidase 6, beta-glucosidase B, beta-glucosidase C, beta-glucosidase I, beta-glucosidase II, beta-glucoside hydrolase, beta-GLYPI, betaG, betagly, BG, BG1, BGA, Bgl, BGL1, BGL1A, BGL1B, Bgl2, Bgl3, bgl3a, Bgl3B, BGL50, BglA, BglB, BglC, BglD5, Bglhi, BglHi2, BglI, BglII, BglP, BGLPf, BglT, BGLU, BGlu1, BGLU21, BGLU22, BGLU23, Bglu3B, bgpA, BGs, BGX1, bifunctional beta-glucosidase/xylosidase, bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase, Cba3, CBG, CC1G_08724, Cel3A, Cel3b, CelB, cellobiase, CGHII, cyanogenic beta-glucosidase, dalcochinase, Dhr1, dhurrinase-1, DV-BG, elaterase, emulsin, esculinase, F1, family-1 beta-glycosidase, FPG, G-II, GBA, GBA2, GBA3, gentiobiase, GH1 beta-glucosidase, GH3 beta-glucosidase, Gh3-4, GI, GII, ginsenoside-hydrolyzing beta-D-glucosidase, Gl-2, Gl-3, Glu1, Glu1b, Glu2, GLU3, GLU4, hCBG, HGT-BG, HiBgl3A, HiBgl3B, HiBgl3C, Hore_15280, ICHG, iridoid beta-glucoside, isoflavone conjugate-hydrolyzing beta-glucosidase, J1, Klotho-related protein, KLrP, KNOUC202beta-gly, lactase, limarase, Lin1840, linamarase, linustatinase, LjBGD2, LjBGD4, LjBGD7, MeBglD2, More, Novozyme 188, oligofurostanoside-specific beta-glucosidase, OsTAGG, p-nitrophenyl beta-glucosidase, PF0073, PGI, PGII, piceid-beta-D-glucosidase, PRGH1, primeverosidase, prunasin hydrolase, reCBG, RuBGX1, SA-bglu, salicilinase, salicinase, SbDhr1, Sde1394, SP188, SSO3039, SYNbglA, T-cell inhibitor, T1, Td2F2, Tnap_0656, Tpbgl, TpBGL1, TpBGL3, Tpebgl3, Tpen_1494, tuberonic acid glucoside (TAG)-hydrolyzing beta-glucosidase, tuberonic acid glucoside beta-glucosidase, Umbgl3B, vicianase, ZM-p60.1, ZmGlu1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.21 beta-glucosidase

Specific Activity

Specific Activity on EC 3.2.1.21 - beta-glucosidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000003
-
in salivary gland
0.000005
-
in midgut
0.000007 - 0.000043
-
mutant enzymes of GD patients
0.000063 - 0.00018
-
obligated GD heterozygotes
0.000081
-
crude extract, at pH 5.0 and 50°C
0.0001 - 0.0005
-
wild-type enzyme
0.002
isoform GLU2, with phloridzin as substrate, at pH 5.5 and 30°C
0.0039
-
substrate: 2-nitrophenyl-beta-D-galactopyranoside, 70°C, pH 5.5
0.005
isoform GLU4, with 2,4-dihydroxy-1,4-benzoxazin-3-one glucoside as substrate, at pH 5.5 and 30°C
0.013
isoform GLU1, with trans-zeatin-O-glucoside as substrate, at pH 5.5 and 30°C
0.041
-
after 506fold purification, at pH 5.0 and 50°C
0.053
isoform GLU2, with daidzin as substrate, at pH 5.5 and 30°C
0.06
pH 5.5, 95°C, substrate: ginsenoside R2
0.065
isoform GLU1, with phloridzin as substrate, at pH 5.5 and 30°C
0.07
-
crude extract
0.071
isoform GLU1, with geniposide as substrate, at pH 5.5 and 30°C
0.077
isoform GLU4, with oleuropein as substrate, at pH 5.5 and 30°C
0.078
isoform GLU4, with genistin as substrate, at pH 5.5 and 30°C
0.08
crude extract, at 70°C
0.086
isoform GLU2, with 2,4-dihydroxy-1,4-benzoxazin-3-one glucoside as substrate, at pH 5.5 and 30°C
0.104
0.149
isoform GLU4, with daidzin as substrate, at pH 5.5 and 30°C
0.15
-
purified enzyme, using cellobiose as substrate
0.23
pH 5.5, 95°C, substrate: ginsenoside Re
0.28
after 163fold purification, using 2-nitrophenyl-beta-D-galactopyranoside as substrate, at 70°C
0.283
isoform GLU1, with 4-nitrophenyl beta-D-cellobioside as substrate, at pH 5.5 and 30°C
0.29
-
crude extract, using 4-nitrophenyl-beta-D-glucopyranoside substrate, at 37°C
0.291
0.3
after 163fold purification, using 2-nitrophenyl-beta-D-glucopyranoside as substrate, at 70°C
0.32
after 163fold purification, using 4-nitrophenyl-beta-D-xylopyranoside as substrate, at 70°C
0.34
isoform GLU4, with dhurrin as substrate, at pH 5.5 and 30°C
0.39
mutant N62Y/Q63K/L231F/V303A/E386G/A412V, substrate cellobiose, pH 3.0, 65°C
0.42
-
crude extract, in 50 mM sodium acetate, pH 5.5, at 37°C
0.472
isoform GLU2, with 4-nitrophenyl beta-D-glucopyranoside as substrate, at pH 5.5 and 30°C
0.54
-
purified enzyme, using 4-nitrophenyl.beta-D-glucopyranoside as substrate
0.58
isoform GLU2, with oleuropein as substrate, at pH 5.5 and 30°C
0.6
pH 5.5, 95°C, substrate: 4-nitrophenyl-alpha-L-rhamnopyranoside
0.75
after 163fold purification, using 4-nitrophenyl-beta-D-lactopyranoside as substrate, at 70°C
0.77
-
purified recombinant enzyme, substrate sophorose
0.949
isoform GLU2, with dhurrin as substrate, at pH 5.5 and 30°C
0.95
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 50°C, mutant enzyme I48W
1
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 50°C, mutant enzyme I48W/I278F/T484Y
1.1
-
purified enzyme
1.2
crude extract, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 6.0 and 90°C
1.3
after 163fold purification, using 4-nitrophenyl-beta-D-cellobioside as substrate, at 70°C
1.6
-
substrate laminaribiose, pH 6.5, 60°C
1.7
after 68fold purification, using 2-nitrophenyl-beta-D-fucopyranoside as substrate, at pH 6.0 and 90°C
1.85
-
thylakoid membrane, substrate deltoside
1.933
isoform GLU1, with genistin as substrate, at pH 5.5 and 30°C
1.97
-
purified recombinant His-tagged enzyme, pH 5.7
10
-
recombinant CBG, hydrolysis of 4-nitrophenyl-beta-D-glucopyranoside
10.4
wild-type, substrate 2-nitrophenyl beta-D-xylopyranoside, pH 3.0, 65°C
10.6
substrate salicin, presence of D-glucose, pH 6.0, 50°C
10.8
after 163fold purification, using cellotetraose as substrate, at 70°C
100
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
102
-
using laminarin as substrate, at pH 5.0 and 50°C
103
-
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 4.8, 60°C, isoform beta-glu 2
103.4
substrate: gentiobiose, pH 5.5, 60°C, wild-type enzyme
105
-
using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 5.0 and 40°C
11
-
purified enzyme
11.34
-
in cell-free extract for laminaribiose, maximum rate of all substrates tested
11.9
-
isoform beta-glucosidase 3, pH 5.0, 50°C
110.5
Bacillus sp. ZJ1308
-
pH 7.0, 50°C
113
after 68fold purification, using 4-nitrophenyl-beta-D-fucopyranoside as substrate, at pH 6.0 and 90°C
113.9
substrate: sophorose, pH 5.5, 55°C
114
-
pH 5.0, 30°C
118
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
119
substrate beta-D-glucopyranoside, pH not specified in the publication, temperature not specified in the publication
119.3
-
cellobiase B
12.5
pH 5.5, 95°C, substrate: 2-nitrophenyl-beta-D-xylopyranoside
12.8
-
native CBG, hydrolysis of 4-nitrophenyl-beta-D-glucopyranoside
124
-
using cellobiose as substrate, at pH 5.0 and 40°C
126
-
latex pellet
129
substrate laminaritetraose, pH not specified in the publication, temperature not specified in the publication
129.4
-
purified enzyme, substrate ginsenoside Rd
129.8
mutant N62Y/Q63K/L231F/V303A/E386G/A412V, substrate 2-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C
13.5
-
purified enzyme
13.8
-
purified recombinant, substrate 4-nitrophenyl-beta-D-glucopyranoside
1300
pH 5.5, 95°C, substrate: 4-nitrophenyl-beta-D-glucopyranoside
130000
pH 5.0, 85°C
131
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
1340
-
-
1345
-
pH 4.8, 50°C
139
-
purified enzyme
14
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
14.1
-
partially purified mutant enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside
14.7
after 163fold purification, using 4-nitrophenyl-beta-D-fucopyranoside as substrate, at 70°C
140.4
-
cellobiase C
140.9
wild-type, substrate 2-nitrophenyl beta-D-galactopyranoside, pH 3.0, 65°C
14000
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-galactopyranoside, pH 7.0, 90°C
1429
-
pH 5.0, 50°C
143
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.0, 50°C
147
-
using beta-gentiobiose as substrate, at pH 5.0 and 40°C
148.5
-
after 164.5fold purification, at pH 5.5 and 50°C
149
thermophilic anaerobic bacterium
-
-
151
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
1520
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 7.0, 90°C
154
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
156.7
purified recombinant His-tagged enzyme
158.8
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 5.5, 60°C
16.2
after 163fold purification, using cellobiose as substrate, at 70°C
16.5
pH 6.0, 60°C
16.8
substrate lactose, presence of D-glucose, pH 6.0, 50°C
160
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week, Phoma aff. glomerata, GenBank Accession: HQ630999
162.8
-
purified enzyme
168
-
inner bark
17.5
-
isoform beta-glucosidase 5, pH 5.0, 50°C
17.9
-
purified enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 0.1 M NaOAc buffer, pH 5.0, at 45°C
170
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
170.5
-
purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside
173
-
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 4.8, 60°C, isoform beta-glu 2
175
substrate cellobiose, pH not specified in the publication, temperature not specified in the publication
176
-
purified enzyme, substrate 4-nitrophenyl beta-D-glucopyranoside, 60°C
179
-
pH 5.5, 90°C, substrate: daidzein
183.4
substrate cellobiose, presence of D-glucose, pH 6.0, 50°C
184.1
-
cellobiase A
185
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
187
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
189
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
19.1
substrate laminaran, pH 5.0, 50°C
19.9
substrate 4-nitrophenyl beta-D-galactopyranoside, presence of D-glucose, pH 6.0, 50°C
190000
-
at pH 4.5 and 60°C
191
-
pH 5.0, 50°C
1917
-
-
194
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
2 - 2.5
-
pH 5.0, 42°C
2.1
purified enzyme, substrate 4-nitrophenyl-alpha-L-arabinoopyranoside, recombinant wild-type enzyme
2.27
-
partially purified wild-type enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside
2.3
purified recombinant enzyme, substrate cellobiose, pH 7.0, 90°C
2.4
Littorina kurila
-
pH 5.4
2.436
isoform GLU1, with daidzin as substrate, at pH 5.5 and 30°C
2.57
-
purified enzyme
2.62
-
purified recombinant His-tagged enzyme, pH 7.0
2.91
crude extract
2.95
-
crude extract, at 50°C, in 50 mM sodium phosphate buffer, pH 6.0, using 4-nitrophenyl-beta-D-glucopyranoside as substrate
20.34
21fold purified recombinant beta-glucosidase
20.6
substrate lichenan. It , pH 5.0, 50°C
202.3
-
hydrolysis of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one-beta-D-glucoside
204
H0HC94
wild-type, substrate cellobiose, pH not specified in the publication, temperature not specified in the publication
207.1
mutant N62Y/Q63K/L231F/V303A/E386G/A412V, substrate 2,4-dinitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C
2088
-
purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside, pH 5.0, 65°C
21.3
-
purified enzyme
211
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
212
substrate laminaribiose, pH not specified in the publication, temperature not specified in the publication
214
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
22
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
22.3
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 5.5, 60°C, mutant enzyme Y509T
226
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
229
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
230
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
24.36
-
after 57.5fold purification, in 50 mM sodium acetate, pH 5.5, at 37°C
24.7
-
for 4-nitrophenyl-beta-D-glucopyranoside
245.9
purified native enzyme
25
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
25 - 57
-
4 different forms
25.1
-
after 36.38fold purification, at 37°C
25.7
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 60°C
250
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks, Phoma sp. aff. herbarum, GenBank Accession: HQ630963
259.9
-
4-nitrophenyl-beta-D-galactoside
266.9
268
substrate gentiobiose, pH not specified in the publication, temperature not specified in the publication
27.2
substrate: 4-nitrophenyl beta-D-fucopyranoside, pH 6.0, 60°C
27.49
-
-
274
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
28
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
281
-
pH 6.0, 35°C
284
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
29
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
290
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
29200
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-fucopyranoside, pH 7.0, 90°C
3 - 8
3.3
-
purified enzyme, substrate piceid
3.36
substrate: sophorose, pH 6.0, 50°C, mutant enzyme I48W/I278F/T484Y
3.49
-
beta-glucosidase purified from honey sac
3.59
substrate: sophorose, pH 6.0, 50°C, mutant enzyme I278F
3.6
purified enzyme, substrate 4-nitrophenyl-beta-D-fucopyranoside, recombinant wild-type enzyme
3.7
substrate: sophorose, pH 6.0, 50°C, mutant enzyme I48W
3.73
-
tuberonic acid beta-D-glucoside as substrate, 4300fold purified protein, pH 4.0, 37°C
3.8
-
substrate cellobiose, pH 6.5, 60°C
30
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
30.7
substrate cellobiose, pH 5.0, 50°C
30400
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside, pH 7.0, 90°C
308
-
purified enzyme
309
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks, Phoma sp. aff. herbarum, GenBank Accession: HQ630963
31
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
31.8
-
isoform beta-glucosidase 6, pH 5.0, 50°C
311.9
purified recombinant His-tagged isozyme TaGlu1a
315
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks, Phoma aff. glomerata, GenBank Accession: HQ630999
32.39
-
after 11fold purification, at 50°C, in 50 mM sodium phosphate buffer, pH 6.0, using 4-nitrophenyl-beta-D-glucopyranoside as substrate
320
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
327
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
33.8
wild-type, substrate cellobiose, pH 3.0, 65°C
333
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
334
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
335
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
336
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
34.083
isoform GLU1, with 2,4-dihydroxy-1,4-benzoxazin-3-one glucoside as substrate, at pH 5.5 and 30°C
342
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
343
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
348
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
359
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
36.2
substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 6.0, 60°C
36.3
substrate: substrate: cellobiose, pH 5.5, 60°C, wild-type enzyme
36.4
substrate 4-nitrophenyl beta-D-glucopyranoside, presence of D-glucose, pH 6.0, 50°C
364
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks, Phoma sp. aff. herbarum, GenBank Accession: HQ630963
376
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
379
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
383
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
387
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
39.4
substrate 4-nitrophenyl beta-D-glucopyranoside, enzyme expression induced by growth on rice bran hemicellulose
4.15
substrate: 4-nitrophenyl beta-D-xylopyranoside, pH 6.0, 60°C
4.46
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 50°C, mutant enzyme T484Y
4.8
-
for 4-nitrophenyl-beta-D-mannopyranoside
4.9
after 68fold purification, using 4-nitrophenyl-beta-D-mannopyranoside as substrate, at pH 6.0 and 90°C
40.3
-
hydrolysis of 2,4-dihydroxy-1,4-benzoxazin-3-one-beta-D-glucoside
405
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
406
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks, Phoma aff. glomerata, GenBank Accession: HQ630999
41.8
purified enzyme, substrate apigenin-7-glucoside, recombinant wild-type enzyme
42.6
-
pH 4.5, 50°C
422
-
substrate polydatin, pH 7.0, 40°C
427
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
43
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
43.3
-
mycelial extract
44
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
440
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
45.6
-
purified enzyme, pH 5.0, 50°C, substrate cellobiose
454
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks, Phoma aff. glomerata, GenBank Accession: HQ630999
46.2
-
purified enzyme, pH 5.0, 50°C, substrate 4-nitrophenyl-beta-D-glucopyranoside
46.3
purified enzyme
47.6
substrate gentobiose, pH 5.0, 50°C
47.8
-
beta-glucosidase 1
47.9
-
pH 5.0, temperature not specified in the publication
48
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
482.8
purified recombinant enzyme
488
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
49.7
purified enzyme, substrate quercetin-4'-glucoside, recombinant wild-type enzyme
493
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
494
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
5.4
-
-
5.64
substrate: sophorose, pH 6.0, 50°C, mutant enzyme T484Y
5.66
-
beta-glucosidase purified from ventriculus
5.7
purified enzyme, substrate 4-nitrophenyl-beta-D-galactopyranoside, recombinant wild-type enzyme
5.814
isoform GLU4, with 4-nitrophenyl beta-D-glucopyranoside as substrate, at pH 5.5 and 30°C
500
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
51
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
51 - 175
-
4 different isoforms, cellobiose as substrate
553
purified recombinant enzyme, substrate 4-nitrophenyl beta-D-glucopyranoside
56.4
substrate: cellobiose, pH 5.5, 55°C
56.7
-
purified enzyme, substrate ginsenoside Rb1
569
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
57
-
using laminaribiose as substrate, at pH 5.0 and 40°C
57.14
-
crude filtrate, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 0.1 M NaOAc buffer, pH 5.0, at 45°C
57.5
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 5.5, 60°C, wild-type enzyme
577
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
584
-
purified beta-glu2
59
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
59.6
-
4-nitrophenyl-beta-D-glucopyranoside
6.1
after 163fold purification, using 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 70°C
6.3
mutant N62Y/Q63K/L231F/V303A/E386G/A412V, substrate 2-nitrophenyl beta-D-galactopyranoside, pH 3.0, 65°C
6.4
mutant N62Y/Q63K/L231F/V303A/E386G/A412V, substrate 2-nitrophenyl beta-D-xylopyranoside, pH 3.0, 65°C
61.7
wild-type, substrate 4-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C
62.6
substrate: daidzin, pH 6.0, 50°C
64.4
-
recombinant enzyme
642
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
65.3
-
beta-glucosidase II
660
purified native enzyme
665
purified recombinant enzyme, substrate cellobiose
672
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
68
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
69
-
using cellotriose as substrate, at pH 5.0 and 40°C
694.6
pH and temperature not specified in the publication
7
-
using beta-gentiobiose as substrate, at pH 5.0 and 40°C
7.08
-
-
7.1
-
isoform beta-glucosidase 4, pH 5.0, 50°C
7.139
isoform GLU1, with dhurrin as substrate, at pH 5.5 and 30°C
7.38
-
purified enzyme
7.86
-
native enzyme
7.87
-
-
7.967
isoform GLU1, with oleuropein as substrate, at pH 5.5 and 30°C
70
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
70.9
-
after 244fold purification, using 4-nitrophenyl -beta-D-glucopyranoside substrate, as at 37°C
717
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
72
-
using alpha-sophorose as substrate, at pH 5.0 and 40°C
72.2
wild-type, substrate 2-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C
73
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
739
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
74.6
purified enzyme
76.7
-
isoform beta-glucosidase 1, pH 5.0, 50°C
77.86
-
purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside
79
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
79 - 102
Sporotrichum pulverulentum
-
5 different forms from culture filtrate
8.5
after 68fold purification, using 4-nitrophenyl-beta-D-galactopyranoside as substrate, at pH 6.0 and 90°C
8.66
isoform GLU1, with 4-nitrophenyl beta-D-glucopyranoside as substrate, at pH 5.5 and 30°C
813
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
82
after 68fold purification, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 6.0 and 90°C
828
pH 5.5, 95°C, substrate: 2-nitrophenyl-beta-D-glucopyranoside
83
-
using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 5.0 and 40°C
84
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
84.3
-
pH 4.5, 37°C
8500
Lenzites trabea
-
-
87
-
using laminaritriose as substrate, at pH 5.0 and 40°C
88
-
beta-glucosidase I
9.1
-
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 6.5, 60°C
9.4
-
after 244fold purification, using 4-nitrophenyl-beta-D-cellobioside substrate, as at 37°C
90
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
91.5
-
for 4-nitrophenyl-beta-D-fucoside
92.9
-
beta-glucosidase 2
94
-
37°C, pH 5.0, release of 4-nitrophenol from 4-nitrophenyl beta-D-glucopyranoside, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
94.6
substrate: sophorose, pH 5.5, 60°C, wild-type enzyme
9400
purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-cellobioside, pH 7.0, 90°C
96
purified recombinant enzyme
97.2
-
purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside
97.7
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.0, 50°C
additional information