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3.2.1.21: beta-glucosidase

This is an abbreviated version!
For detailed information about beta-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.21

Reaction

celloheptaose
+ 6 H2O = 7 beta-D-glucose

Synonyms

1,4-beta-glucosidase, 3BGlu6, 4BGlu12, A1, amygdalase, amygdalin hydrolase, amygdalinase, arbutinase, aryl beta-glucosidase, aryl-beta-D-glucosidase, aryl-beta-glucosidase, AT5A_15831, beta-1,6-glucosidase, beta-D-glucosidase, beta-D-glucosidase F1, beta-D-glucoside glucohydrolase, beta-Glcase, beta-glu, beta-Glu x, beta-glu1, beta-glu2, beta-glucosidase, beta-glucosidase 1, beta-glucosidase 6, beta-glucosidase B, beta-glucosidase C, beta-glucosidase I, beta-glucosidase II, beta-glucoside hydrolase, beta-GLYPI, betaG, betagly, BG, BG1, BGA, Bgl, BGL1, BGL1A, BGL1B, Bgl2, Bgl3, bgl3a, Bgl3B, BGL50, BglA, BglB, BglC, BglD5, Bglhi, BglHi2, BglI, BglII, BglP, BGLPf, BglT, BGLU, BGlu1, BGLU21, BGLU22, BGLU23, Bglu3B, bgpA, BGs, BGX1, bifunctional beta-glucosidase/xylosidase, bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase, Cba3, CBG, CC1G_08724, Cel3A, Cel3b, CelB, cellobiase, CGHII, cyanogenic beta-glucosidase, dalcochinase, Dhr1, dhurrinase-1, DV-BG, elaterase, emulsin, esculinase, F1, family-1 beta-glycosidase, FPG, G-II, GBA, GBA2, GBA3, gentiobiase, GH1 beta-glucosidase, GH3 beta-glucosidase, Gh3-4, GI, GII, ginsenoside-hydrolyzing beta-D-glucosidase, Gl-2, Gl-3, Glu1, Glu1b, Glu2, GLU3, GLU4, hCBG, HGT-BG, HiBgl3A, HiBgl3B, HiBgl3C, Hore_15280, ICHG, iridoid beta-glucoside, isoflavone conjugate-hydrolyzing beta-glucosidase, J1, Klotho-related protein, KLrP, KNOUC202beta-gly, lactase, limarase, Lin1840, linamarase, linustatinase, LjBGD2, LjBGD4, LjBGD7, MeBglD2, More, Novozyme 188, oligofurostanoside-specific beta-glucosidase, OsTAGG, p-nitrophenyl beta-glucosidase, PF0073, PGI, PGII, piceid-beta-D-glucosidase, PRGH1, primeverosidase, prunasin hydrolase, reCBG, RuBGX1, SA-bglu, salicilinase, salicinase, SbDhr1, Sde1394, SP188, SSO3039, SYNbglA, T-cell inhibitor, T1, Td2F2, Tnap_0656, Tpbgl, TpBGL1, TpBGL3, Tpebgl3, Tpen_1494, tuberonic acid glucoside (TAG)-hydrolyzing beta-glucosidase, tuberonic acid glucoside beta-glucosidase, Umbgl3B, vicianase, ZM-p60.1, ZmGlu1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.21 beta-glucosidase

Purification

Purification on EC 3.2.1.21 - beta-glucosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
117fold to homogeneity from fresh leaves by chilled acetone and ammonium sulfate precipitation, ion exchange chromatography and gel filtration
-
18fold by ammonium sulfate fractionation, ion exchange and adsorption chromatography, and gel filtration to homogeneity
-
a single step concanvalin A affinity chromatography for native PRGH1 improves the yield and reduced the purification time
acetone precipitation, DEAE-cellulose
Thermochaetoides thermophila
-
affinity column chromatography
-
after growth on 2% microcrystalline cellulose
-
after growth on cellulose
-
ammonium sulfate precipitation and Sepharose-4B-L-tyrosine-1-naphthylamine column chromatography
-
ammonium sulfate precipitation, butyl Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
ammonium sulfate precipitation, butyl-Toyopearl 650M column chromatography, DEAE-Toyopearl 650M column chromatography, TSK gel filtration
-
ammonium sulfate precipitation, Concanavalin A-Sepharose column chromatography, Shodex-phenyl HIC-814 column chromatography, and Sephacryl S-200 gel filtration
-
ammonium sulfate precipitation, Macro-Prep methyl HIC column chromatography, Source 15 Q column chromatography, and Sephacryl S-300 gel filtration
-
ammonium sulfate precipitation, Sephadex G-200 gel filtration, and DEAE-cellulose column chromatography
-
ammonium sulfate precipitation, Source 15Q column chromatography, Biogel P4 gel filtration, and phenyl-Superose column chromatography
-
ammonium sulfate precipitation, Source 15Q column chromatography, Biogel P4 gel filtration, and Source 15 isopropyl column chromatography
ammonium sulfate, Butyl-Toyopearl, hydroxyapatite, Q-Sepharose, Sephacryl S-300
-
ammonium sulfate, DEAE-Sephadex, CM-Sephadex
-
ammonium sulfate, DEAE-Sepharose, hydroxyapatite
-
ammonium sulfate, gel filtration, anion-exchange, gel filtration
-
ammonium sulfate, gel filtration, anion-exchange, reversed-phase HPLC
-
ammonium sulfate, ion exchange, gel filtration
-
ammonium sulfate, Q-Sepharose, DEAE-Toyopearl, t-Butyl
-
ammonium sulfate, Sephadex G-100, DEAE-Sepharose,CL-6B, DEAE-Sephacel
-
ammonium sulfate, Source Q, Macrosep, Superdex 200, hydroxyapatite
-
by chromatographic steps to over 95% purity
-
ca. 4300fold, yield of 1.1%, ammonium sulfate precipitation, CM-Sepharose, ConA Sepharose, Superdex 200, Resource S, hydroxyapatite
-
CM-Sephadex C-50 gel filtration, DEAE-Sephadex A-50 gel filtration, Sephadex G-200 gel filtration, and Con A Sepharose column chromatography
-
cryoprecipitation, Accell Plus CM, Superdex 200
-
DEAE A-50, Sephacryl S-200, Mono Q, TSK DEAE-5PW, Superose 12
-
DEAE Sepharose column chromatography, ammonium sulfate precipitation, Sephacryl S-100 gel filtration, and phenyl-Sepharose column chromatography
-
DEAE Sepharose column chromatography, MonoQ column chromatography, and hydroxyapatite column chromatography
DEAE-Sephacel, CM-Sepharose, Superdex, Toyopearl
-
DEAE-Sepharose, Phenyl-Sepharose, Sephacryl S-300 HR
-
DEAE-Sepharose, Superose, CM-sepharose, Mono S, hydroxyapatite, Superose 12
-
DEAE-Sepharose, TSK-DEAE-5PW, TSK 3000 SW
-
DEAE-Sepharose, Ultrogel AcA 44, Mono P
-
enzymes from wild-type strain NIAB 442, 17fold, and the deoxyglucose- and streptomycin-resistant mutant strain, 23fold, to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
ethanol precipitation, Sephadex G-100, Q-Sepharose
-
further purification of a cellulase preparation, chromatography steps, to homogeneity
-
further purification of commercially available enzyme preparation, to homogeneity, chromatography steps
-
heat treatment and His-Trap affinity chromatography
Hi-Trap column chromatography, gel filtration
HisTrap column chromatography
-
HPGPLC column chromatography, Sephacryl S-200 gel filtration and DEAE Sephadex gel filtration
-
immobilized metal (Co2+) affinity chromatography on Talon resin
isoform A and B
-
isolation of isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant
-
Mono Q column chromatography
-
native beta-glu2 60fold to homogeneity by ammonium sulfate precipitation, gel filtration, anion exchange chromatography, and chromatofocusing
-
native CBG, cation-exchange chromatography, Octyl-Sepharose, chromatofocusing, gel filtration, recombinant CBG, Octyl-Sepharose
-
native enzyme 10.2fold to homogeneity by anion exchange chromatography, ultrafiltration, and affinity chromatography using 4-aminobenzyl, 1-thio-beta-D-galactopyranoside
-
native enzyme 105fold to homogeneity by anion exchange chromatography and gel filtration
-
native enzyme 12fold in a two-step process involving hydrophobic interaction chromatography, with an additional anion exchange chromatography step
native enzyme 144fold by ammonium sulfate fractionation, anion exchange chromatography, and affinity chromatography on microcrystalline cellulose, to homogeneity, method optimization, overview
-
native enzyme 217fold from flower to homogeneity by anion exchange and hydrophobic interaction chromatography, gel filtration, concanavalin A affinity chromatography and again anion exchange chromatography
-
native enzyme 23.6fold from crude culture extract by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, ultrafiltration, again gel filtration, followed by hyrophobic interaction chromatography and ultrafiltration
-
native enzyme 35.5fold to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange chromatography, gel filtration, and ultrafiltration
-
native enzyme 36fold from petals to homogeneity by ammonium sulfate fractionation, two steps of ion exchange chromatography, and ultrafiltration
-
native enzyme 45fold to homogeneity by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration
-
native enzyme 46.3fold to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange chromatography, gel filtration, and ultrafiltration
-
native enzyme 50fold from culture filtrate by ammonium sulfate precipitation, cation-exchange and hydrophobic interaction chromatography, and gel filtration
-
native enzyme 626fold from acetone powder of viscera by two steps of anion exchange chromatography, hydrophobic interaction chromatography, and two steps of gel filtration
-
native enzyme from culture supernatant to homogeneity by anion exchange chromatography and gel filtration
-
native enzyme from roots, 4199fold by ammonium sulfate fractionation, anion and cation exchange chromatography, hydrophobic interaction chromatography, gel filtration, and heparin affinity chromatography, to homogeneity
native enzyme partially
-
native enzyme partially from juice of ripe fruits by ammonium sulfate fractionation
-
native enzyme to homogeneity
Melanocarpus sp.
-
native extracellular enzyme 130.3fold from culture supernatant by ultrafiltration, anion exchange chromatography and gel filtration to homogeneity
-
native extracellular isozyme by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
native large isozyme 3.7fold by successive gel filtration
-
native piceid-beta-D-glucosidase 12.6fold by ammonium sulfate fractionation, anion exchange chromatography, and freeze drying
-
native wild-type and mutant enzymes partially by anion exchange chromatography and gel filtration 29fold and 16fold, respectively
-
near homogeneity, chromatography techniques
-
near homogeneity, density gradient centrifugation
-
Ni+2-nitrilotriacetic acid column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and Mono Q column chromatography
Ni-NTA resin column chromatography and Mono-S column chromatography
partial
partially, subcellular fractionation, sucrose density gradient centrifugation
-
purification from meal
-
purified 138.85fold by ammonium sulfate precipitation, DE-22 ion exchange and Sephadex G-150 gel filtration chromatography
-
purified by salting out with ammonium sulfate and using specifically designed Sepharose-4B-l-tyrosine-1-naphthylamine hydrophobic interaction chromatography. Purification: 155fold, enzyme yield: 54%
-
purified in the presence and absence of 2-deoxy-D-glucose by ultrafiltration, DEAE Sephadex A-50 gel filtration, high-performance gel-permeation liquid chromatography, and Sephacryl S-200 gel filtration
-
Q Sepharose column chromatography, Superdex 75 gel filtration, Resource Q column chromatography, Source 30S column chromatography, Superdex 200 gel filtration, and phenyl Sepharose column chromatography
Q-Sepharose resin column chromatography and Sephacrly S-100 gel filtration
-
quartenary amine column, Phenyl Sepharose
-
recombinant and wild-type beta-glucosidase
recombinant beta-glucosidase purified by gel filtration to apparent homogeneity, 21fold with an overall enzyme yield of 6.8%
recombinant Bgl3
-
recombinant BglA
-
recombinant BglA 52.9fold from Escherichia coli by ammonium sulfate fractionation, ion exchange and adsorption chromatography, and gel filtration
recombinant Cel3A
recombinant enzyme 1112.2fold from Spodoptera frugiperda Sf9 cells by ammonium sulfate fractionation, gel filtration and anion exchange chromatography, cleavage of the signal peptide
recombinant enzyme 16fold to homogeneity by ion exchange chromatography and gel filtration
recombinant enzyme 23.3fold from yeast by immobilized metal-ion affnity chromatography, following hydrophobic interaction chromatography, insertion of a polyhistidine-tag either after the N-terminal alpha-factor signal sequence or at the C-terminus fails to assist in purification by immobilized metal-ion affnity chromatography due to post-translational processing at both termini
-
recombinant enzyme 24fold from Trichoderma reesei strain Rut-C30 by ultrafiltration, two steps of gel filtration, and ion exchange chromatography
recombinant enzyme from Escherichia coli by immobilized metal-affinity chromatography and removal of the tags, followed by cation exchange chromatography and gel filtration to about 95% purity
recombinant enzyme from Escherichia coli strain BL21 Codon-Plus
recombinant enzyme from Escherichia coli strain JM109
-
recombinant extracellular His6-tagged enzyme from Escherichia coli strain JM101 culture supernatant by cobalt affinity chromatography, cleavage of the fusion protein with thrombin
recombinant hCBG
-
recombinant His-tagged Dhr1 and mutant E189D from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged enzyme 6.4fold from Escherichia coli to homogeneity by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli by heat treatment at 75°C, and nickel affinity chromatography
-
recombinant His-tagged enzyme lacking the potential signal sequence from Pichia pastoris strain KM71H by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography
recombinant His-tagged Glu1b from Escherichia coli by nickel affinity chelating chromatography, ultrafiltration, and gel filtration
-
recombinant His-tagged isozyme BGL1A from Escherichia coli by ammonium sulfate fractionation, hydrophobic interaction chromatography, and nickel affinity chromatography
recombinant His-tagged isozyme BGL1A from Escherichia coli by anion exchange and affinity chromatography, and gel filtration
recombinant His-tagged isozyme BGL1B from Escherichia coli by ammonium sulfate fractionation, hydrophobic interaction chromatography, and nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and two steps of anion exchange chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and selenomethionine-labeld enzymes from Escherichia coli by immobilized metal-ion affinity chromatography column
-
recombinant isozyme BglB from Escherichia coli to homogeneity
recombinant N-terminally His-tagged wild-type and chimeric mutant enzymes from Escherichia coli by nickel affinity chromatography
recombinant N-terminally His6-tagged cytosolic isozyme from Sf9 insect cells by nickel affinity and ion exchange chromatography to homogeneity
-
recombinant wild-type and mutant enzymes from Escherichia coli by metal affinity chromatography and gel filtration to homogeneity
recombinant wild-type and mutant isozyme TaGlu1a from Escherichia coli by metal affinity chromatography and gel filtration to homogeneity
Sephacryl S-300, Q-Sepharose
-
Sepharose 6 immobilized metal-ion affinity chromatography on cobalt
to homogeneity by ammonium sulfate fractionation, gel filtration, ion exchange chromatography, and adsorption chromatography
-
to homogeneity, 2step chromatography
to homogeneity, 3step chromatography
-
to homogeneity, 4 different forms, chromatography steps
-
to homogeneity, as fusion protein by affinity chromatography
-
to homogeneity, chromatography
-
to homogeneity, chromatography steps
to homogeneity, chromatography steps, beta-glucosidases I, III and IV
-
to homogeneity, chromatography steps, isoelectric focusing
-
to homogeneity, chromatography steps, preparative gel electrophoresis
thermophilic anaerobic bacterium
-
to homogeneity, chromatography steps, wild-type and enzyme from mutant strain
-
to homogeneity, chromatography techniques
to homogeneity, chromatography techniques, 2 isoforms
-
to homogeneity, chromatography techniques, isoelectric focusing
-
to homogeneity, chromatography techniques, isoform GB-1
-
to homogeneity, crude enzyme preparation by further chromatography steps
-
to homogeneity, extracellular form, 2step chromatography
-
to homogeneity, five different extracellular forms, chromatography steps
Sporotrichum pulverulentum
-
to homogeneity, further preparation of commercial cellulase preparation
-
to homogeneity, HPLC chromatography
-
to homogeneity, NAD+ affinity chromatography
-
to homogeneity, native and recombinant enzyme
-
to homogeneity, preparative electrophoresis, 2 isoforms
-
to homogeneity, preparative isoelectric focusing, 1step chromatography
-
to homogeneity, recombinant enzyme
Tpen_1494 is ligated into p6xHis119 downstream of the BLMA promoter to create p6xHisTpbgl. Recombinant Tpbgl, with eight additional amino acids (Met-Glu-(His)6) at the N-terminus, is expressed in Escherichia coli harboring p6xHisTpbgl
ultrafiltration, Ultrogel, DEAE-Sepharose, chromatofocusing
-
using a glutathione-Sepharose 4B column
-
using ammonium sulfate precipitation followed by ion-exchange chromatography
Halalkalibacterium halodurans
-
using CM-sepharose chromatography
-
using gel filtration and ion-exchange chromatography
using Ni-NTA chromatography
wild-type and mutant enzyme R170A/R220A/Y227F