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106000
-
detergent-solubilized form, density-gradient centrifugation
107000
-
beta-glucosidase 3, gel filtration
109000
-
x * 109000, SDS-PAGE
111000
-
x * 111000, SDS-PAGE
121000
-
x * 121000, SDS-PAGE
125900
-
HPGPLC column chromatography
128000
-
SDS-PAGE, gel filtration
129000
-
density gradient centrifugation, isoelectric focusing
132000
-
beta-glucosidase 2, gel filtration
138000
-
x * 138000, SDS-PAGE
140000
the membrane fractions of cells grown in presence of cellobiose contain two isozymes of 75 and 140 kDa, SDS-PAGE
147000
-
for PGII, gel filtration
157000
-
x * 157000, SDS-PAGE
165000 - 182000
Sporotrichum pulverulentum
-
gel filtration, five different forms in culture filtrate
175000
-
x * 175000, SDS-PAGE
176000
-
recombinant enzyme
18000
-
gel filtration, isoform II
182000
beta-glucosidase TpBGL3, dynamic light scattering at pH 6
193000
-
native protein, gel filtration
225000 - 230000
-
recombinant mutant enzyme
23600
wild-type enzyme tetramer, gel filtration
247000
-
for PGI, gel filtration
26000
-
1 * 42000 + 1 * 26000, SDS-PAGE
260000
native PAGE and gel filtration, recombinant enzyme
260000 - 300000
-
gel filtration
28000
-
2 * 28000, SDS-PAGE
290000
-
beta-glucosidase C, +/-10%, determined by Sephadex G 200
311000
-
gel filtration, native PAGE
32197
x * 32197, calculated
39100
-
2 * 39100, SDS-PAGE
40000
Thermochaetoides thermophila
-
gel filtration
41000
-
1 * 41000, SDS-PAGE
43000 - 50000
thermophilic anaerobic bacterium
-
gel filtration, SDS-PAGE
440000
-
native enzyme, tetramer, gel filtration
45700
-
beta-glucosidase III
46000
recombinant isozyme BGL1A, gel filtration
46500
-
recombinant His-tagged enzyme, gel filtration
47000 - 48000
-
native gradient PAGE
48000
-
gel filtration, SDS-PAGE
48642
-
1 * 48642, deduced from amino acid composition, SDS-PAGE
49900
calculated from cDNA
49951
x * 49951, calculated from amino acid sequence
50000 - 58000
-
gel filtration
51300
-
deduced from amino acid composition
51600
Halalkalibacterium halodurans
-
MALDI-TOF
51900
-
1 * 51900, SDS-PAGE
52000
1 * 52000, isozyme BglB, SDS-PAGE
52280
-
non-denaturating gradient PAGE
52600
-
deduced from amino acid composition, SDS-PAGE
54600
deduced from amino acid composition
56100
dynamic light scattering
56200
-
mass spectrometry
56691
-
x * 56691, calculated from sequence
57070
subunit, MALDI-TOF mass spectrometry
57300
-
high-performance gel-permeation liquid chromatography
57346
-
1 * 55000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 57346, mass spectrometry
57600
-
beta-glucosidase IC, gel filtration
59800
BGLU 22, calculated from seuqence
60600
-
1 * 60600, SDS-PAGE
60680
2 * 60680, deduced from nucleotide sequence
61100
-
4 * 61100, SDS-PAGE, for PGI
62000
-
x * 62000, SDS-PAGE
63000
SDS-PAGE, recombinant glycosylated protein
64000
-
x * 64000, SDS-PAGE
64200
-
1 * 64200, SDS-PAGE
6688
-
MALDI-TOF mass spectrometry, in the presence of 2-deoxy-D-glucose
69000
-
beta-glucosidase II, SDS-PAGE
72000
-
x * 72000, SDS-PAGE
77000
-
about, piceid-beta-D-glucosidase, gel filtration
77800
x * 77800, SDS-PAGE
78280
calculated from sequence
79000
-
4 * 79000, SDS-PAGE
83000
-
2 * 83000, recombinant enzyme
84000
beta-glucosidase TpBGL3, SDS-PAGE
85000 - 105000
-
gel filtration, SDS-PAGE
88000
-
cellobiase A, SDS-PAGE
89000
-
1 * 89000, SDS-PAGE
89620
calculated from amino acid sequence
91400
-
2 * 91400, deduced from nucleotide sequence
91457
x * 91457, deduced from nucleotide sequence
93000
-
x * 93000, SDS-PAGE
93400
3 * 93400, SDS-PAGE
93418
3 * 93418, calculated from sequence
95000
-
4 * 95000, SDS-PAGE
96500
-
2 * 96500, SDS-PAGE
96870
calculated from sequence
97000
4 * 97000, recombinant enzyme, SDS-PAGE
97120
calculated from sequence
98000
-
x * 98000, SDS-PAGE, both isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant
100000
-
-
100000
-
beta-glucosidase II, gel filtration
100000
-
x * 100000, SDS-PAGE
100000
-
x * 100000, SDS-PAGE
101000
calculated from amino acid sequence
101000
-
2 * 101000, SDS-PAGE
102000
Melanocarpus sp.
-
gel filtration
102000
-
2 * 102000, SDS-PAGE
102000
-
3 * 102000, SDS-PAGE
102000
beta-glucosidase TpBGL3, dynamic light scattering at pH 6
110000
-
gel filtration
110000
-
subunit, SDS-PAGE
110000
-
beta-glucosidase II, gel filtration
110000
-
large isozyme, gel filtration
110000
-
2 * 110000, SDS-PAGE
110000
-
2 * 110000, SDS-PAGE
110000
-
3 * 110000, SDS-PAGE
113000
-
gel filtration
113000
beta-glucosidase TpBGL1, dynamic light scattering at pH 4
114000
native enzyme, gel filtration
114000
-
beta-glucosidase II, SDS-PAGE
115000
SDS-PAGE
115000
-
4 * 115000, SDS-PAGE
116000
-
SDS-PAGE
116000
-
4 * 116000, SDS-PAGE
116000
x * 116000, SDS-PAGE, wild-type BGL
118000
-
SDS-PAGE
118000
-
2 * 118000, SDS-PAGE
120000
-
SDS-PAGE
120000
His-tagged recombinant enzyme, native PAGE
120000
-
beta-glucosidase B, +/-10%, determined by 5-20% linear sucrose gradient centrifugation
120000
-
2 * 120000, SDS-PAGE
120000
-
x * 120000, SDS-PAGE
120000
1 * 120000, SDS-PAGE
120000
-
2 * 120000, SDS-PAGE, isoform I
120000
-
3 * 120000, SDS-PAGE
125000
-
SDS-PAGE
125000
-
beta-glucosidase B, +/-10%, determined by Sephadex G 200
130000
-
gel filtration
130000
-
x * 130000, SDS-PAGE
130000
2 * 130000, recombinant enzyme, SDS-PAGE
130000
-
x * 130000, calculated, x * 130000, SDS-PAGE, and x * 230000, SDS-PAGE of enzyme purified in presence of Ca2+ or enzyme in crude extract
133000
-
beta-glucosidase 1, gel filtration
133000
x * 133000, SDS-PAGE, recombinant BGL
135000
-
gel filtration
135000
-
native PAGE, SDS-PAGE
135000
-
beta-glucosidase I, gel filtration
136000
-
beta-glucosidase 3, gel filtration
136000
mutant enzyme R170A/R220A/Y227F, dimer, gel filtration
167000
-
1 * 167000, SDS-PAGE
167000
beta-glucosidase TpBGL1, dynamic light scattering at pH 4
170000
-
gel filtration
170000
-
about, gel filtration
200000
-
gel filtration
200000
Littorina kurila
-
x * 200000, SDS-PAGE
210000
-
gel filtration
220000
-
gel filtration
220000
-
recombinant wild-type enzyme
220000
-
beta-glucosidase C, +/-10%, determined by 5-20% linear sucrose gradient centrifugation
230000
-
gel filtration, isoform I
230000
-
x * 130000, calculated, x * 130000, SDS-PAGE, and x * 230000, SDS-PAGE of enzyme purified in presence of Ca2+ or enzyme in crude extract
240000
-
gel filtration
27000
-
gel filtration
27000
-
2 * 27000, SDS-PAGE
275000
-
gel filtration
275000
-
disc gel electrophoresis
300000
-
gel filtration
300000
-
yeast foam strain
300000
-
gel filtration, isoform beta-glucosidase 1
313000
gel filtration
313000
-
intracellular enzyme
320000
Lenzites trabea
-
gel filtration
320000
-
gel filtration, recombinant and native enzyme
33000
-
estimated from SDS-PAGE
33000
-
1 * 33000, SDS-PAGE
330000
-
gel filtration
330000
-
native electrophoresis
380000
-
gel filtration
380000
recombinant enzyme, gel filtration
380000
-
native electrophoresis, enzyme from mutant strain
42000
-
x * 42000, SDS-PAGE
42000
-
1 * 42000 + 1 * 26000, SDS-PAGE
43000
-
gel filtration
43000
Thermochaetoides thermophila
-
1 * 43000, SDS-PAGE
460000
-
native-PAGE
460000
-
gel filtration, isoform beta-glucosidase 2
460000
-
gel filtration, isoform beta-glucosidase 3
49000
-
gel filtration
49000
-
gel filtration, SDS-PAGE
49000
-
x * 49000, SDS-PAGE
50000
-
-
50000
recombinant enzyme, gel filtration
50000
mutant enzyme R170A/R220A/Y227F/R459G, monomer, gel filtration
50000
-
4 * 50000, SDS-PAGE
50000
-
4 * 50000, SDS-PAGE
50000
x * 50000, SDS-PAGE
50000
-
1 * 50000, SDS-PAGE
50000
x * 66000, tagged recombinant enzyme, SDS-PAGE, x * 50000, detagged recombinant enzyme, SDS-PAGE
51500
SDS-PAGE
53000
-
gel filtration, native PAGE
53000
-
small isozyme, gel filtration
53000
x * 53000, SDS-PAGE
53000
-
1 * 53000, SDS-PAGE
53000
-
1 * 53000, SDS-PAGE, recombinant CBG
53000
1 * 53000, recombinant isozyme BGL1A, SDS-PAGE
53000
x * 53000, recombinant His-tagged isozyme BGL1A, SDS-PAGE
54000
-
gel filtration
54000
2 * 54000, SDS-PAGE
54000
x * 54000, CelG, SDS-PAGE
54000
beta-glucosidase TpBGL1, SDS-PAGE
55000
SDS-PAGE
55000
-
1 * 55000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 57346, mass spectrometry
55000
-
x * 65000 + x * 55000, SDS-PAGE
55000
2 * 55000, SDS-PAGE after heating at 95°C,mutant enzyme R170A/R220A/Y227F
57000
-
SDS-PAGE
57000
2 * 57000, SDS-PAGE
57000
-
x * 57000, SDS-PAGE
58000
-
SDS-PAGE
58000
-
alpha=39000 Da, beta=19000 Da, chromatography on P-100 and S-300 columns
58000
2 * 58000, SDS-PAGE
58000
-
1 * 58000, SDS-PAGE
59000
-
gel filtration
59000
-
gel filtration, SDS-PAGE
59700
BGLU 21, calculated from sequence
59700
BGLU 23, calculated from sequence
60000
-
gel filtration
60000
-
2 * 60000, SDS-PAGE
60000
-
1 * 60000, SDS-PAGE
60000
-
4 * 60000, SDS-PAGE
60000
-
1 * 80000 + 1 * 60000, SDS-PAGE
60000
-
x * 60000, recombinant enzyme, SDS-PAGE
60000
x * 60000, recombinant His-tagged enzyme, SDS-PAGE
60000
x * 60000, recombinant His-tagged isozyme BGL1B, SDS-PAGE
60000
x * 60000, SDS-PAGE, recombinant mutated beta-glucosidase
65000
-
gel filtration
65000
-
1 * 65000, SDS-PAGE
65000
-
x * 65000 + x * 55000, SDS-PAGE
66000
-
PAGE
66000
x * 66000, tagged recombinant enzyme, SDS-PAGE, x * 50000, detagged recombinant enzyme, SDS-PAGE
68000
-
SDS-PAGE
68000
-
1 * 68000, SDS-PAGE
70000
-
gel filtration
70000
-
4 * 70000, SDS-PAGE
71000
-
beta-glucosidase I, SDS-PAGE
71000
-
cellobiase C, SDS-PAGE
71000
mutant enzyme R170A/R220A/Y227F/R448E, monomer, gel filtration
71000
-
2 * 71000, SDS-PAGE, for PGII
74000
-
gel filtration
74000
-
beta-glucosidase I, SDS-PAGE
75000
-
SDS-PAGE
75000
the membrane fractions of cells grown in presence of cellobiose contain two isozymes of 75 and 140 kDa, SDS-PAGE
76000
-
gel filtration
80000
-
80000
-
cellobiase B, SDS-PAGE
80000
-
chimeric beta-glucosidases No. 6 and No. 8 type, SDS-PAGE
80000
-
chimeric beta-glucosidases No. 6 and No. 8 type, SDS-PAGE
80000
purified RuBGX1-6His
80000
-
2 * 80000, SDS-PAGE
80000
-
4 * 80000, SDS-PAGE
80000
-
4 * 80000, SDS-PAGE
80000
-
1 * 80000 + 1 * 60000, SDS-PAGE
80000
-
gel filtration, isoform beta-glucosidase 5
82000
-
gel filtration, SDS-PAGE
82000
-
2 * 82000, SDS-PAGE
85000
-
gel filtration
85000
-
gel filtration, SDS-PAGE
85000
-
2 * 85000, SDS-PAGE
85000
-
1 * 85000, SDS-PAGE
85000
2 * 85000, SDS-PAGE, difference to deduced molecular weight is due to glycosylation
90000
-
gel filtration
90000
-
beta-glucosidases 1 and 2, gel filtration
90000
-
4 * 90000, SDS-PAGE
90000
-
4 * 90000, SDS-PAGE
90000
-
4 * 90000, SDS-PAGE
92000
-
beta-glucosidase 4, gel filtration
92000
Melanocarpus sp.
-
1 * 92000, SDS-PAGE
94000
-
SDS-PAGE
94000
-
2 * 94000, SDS-PAGE
96000
-
beta-glucosidase II, gel filtration
96000
mutant enzyme R170A/R220A/Y227F/R449R, monomer, gel filtration
additional information
formation of both homo- and heterohexamers by the isozyme
additional information
formation of both homo- and heterohexamers by the isozyme
additional information
formation of both homo- and heterohexamers by the isozyme
additional information
formation of both homo- and heterohexamers by the isozymes
additional information
formation of both homo- and heterohexamers by the isozymes
additional information
formation of both homo- and heterohexamers by the isozymes
additional information
dimer structure, SAXS scattering and energetic analysis