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0.45
(1S,5S)-3-methylidene-6,8-bis[(1S)-1-phenylethyl]-6,8-diazabicyclo[3.2.2]nonane-7,9-dione
-
-
0.00515
(2R,3R,4R,5S)-1-butyl-2-(hydroxymethyl)piperidine-3,4,5-triol
pH 4.0, 60°C
0.58
(2S,5S)-2-benzyl-3,11-bis[(1S)-1-phenylethyl]-2,3,5,6-tetrahydro-5,2-(epiminomethano)-3-benzazocine-4,12(1H)-dione
-
-
2.2
(2S,5S)-2-methyl-3,11-bis[(1S)-1-phenylethyl]-2,3,5,6-tetrahydro-5,2-(epiminomethano)-3-benzazocine-4,12(1H)-dione
-
-
0.535
(2S,5S)-3,11-bis[(1S)-1-phenylethyl]-2,3,5,6-tetrahydro-5,2-(epiminomethano)-3-benzazocine-4,12(1H)-dione
-
-
0.15 - 0.29
1-deoxynojirimycin
0.41
2-nitrophenyl-beta-D-fucopyranoside
-
pH 7.3, 40°C, versus substrate 4-nitrophenyl beta-D-glucopyranoside
0.058 - 0.223
3,8-dibutylamino-7,8-dideoxy-5-octylimino-6-oxa-3-epi-australine
17.9
4-nitrophenyl beta-D-glucopyranoside
-
pH and temperature not specified in the publication
0.0064
4-[4-(cyanomethoxy)phenyl]-4-oxo-N-{3-[(2R,3R,4R,5S)-3,4,5-trihydroxy-2-(hydroxymethyl)piperidin-1-yl]propyl}butanamide
-
-
0.025 - 0.367
7,8-dideoxy-3,8-didodecylamino-5-octylimino-6-oxa-3-epi-australine
0.012 - 0.441
7,8-dideoxy-3,8-dioctylamino-5-octylimino-6-oxa-3-epi-australine
0.0096 - 0.484
7,8-dideoxy-3-hydroxy-8-octylamino-5-octylimino-6-oxa-3-epi-australine
0.0029 - 0.056
7,8-dideoxy-8-dodecylamino-3-hydroxy-5-octylimino-6-oxa-3-epi-australine
0.051 - 0.32
8-butylamino-7,8-dideoxy-3-hydroxy-5-octylimino-6-oxa-3-epi-australine
0.00321
Ag+
-
pH 5.5, 37°C
0.29 - 200
beta-D-glucose
0.000429
Cd2+
-
pH 5.5, 37°C
0.14 - 19.6
cellotetraose
9
conduritol beta-epoxide
pH 4.0, 60°C
0.000226
Cu2+
-
pH 5.5, 37°C
260
D-allose
-
25°C, pH 4.5
0.0083
D-delta-gluconolactone
330
D-glucoheptose
-
pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside
0.016 - 0.49
D-glucono-1,5-lactone
0.012 - 5
D-glucono-delta-lactone
0.008 - 0.4
D-gluconolactone
130
D-glucuronamide
-
pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside
160
D-sorbose
-
pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside
1.4
decyl-beta-D-glucopyranoside
-
vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0
0.021 - 2.2
delta-gluconolactone
0.004
deoxynojirimycin
-
pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside
0.3
gluconic acid delta-lactone
wild-type, pH 6.0, 20°C
0.0005 - 23
glucono-delta-lactone
0.038 - 4.7
gluconolactone
0.7 - 21.8
heptyl beta-D-glucoside
5.9
heptyl-beta-D-glucopyranoside
-
vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0
1.57 - 44.9
hexyl beta-D-glucoside
9.8
hexyl-beta-D-glucopyranoside
-
vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0
0.0041
isofagomine D-tartrate
wild-type, pH 6.0, 20°C
360
L-idose
-
pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside
10
linustatin
-
pH 6, 30°C
320
maltose
-
25°C, pH 4.5
5.1 - 15.6
methyl beta-D-glucoside
440
myo-inositol
-
pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside
0.0013
N-{3-[4-(cyanomethoxy)benzoyl]benzoyl}propylamido-1-deoxynojirimycin
-
-
5
neolinustatin
-
pH 6, 30°C
0.000104
Ni2+
-
pH 5.5, 37°C
0.00026
Nojirimycin
-
pH 5.4, 50°C
0.13 - 5.9
nonyl beta-D-glucoside
1.7
nonyl-beta-D-glucopyranoside
-
vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0
0.42 - 11
octyl beta-D-glucoside
3.1 - 4
octyl-beta-D-glucopyranoside
0.4
p-nitrophenyl-beta-D-glucopyranoside
-
vs. cellobiose, 30°C, pH 6.0
0.0126
Pb2+
-
pH 5.5, 37°C
18
pentyl-beta-D-glucopyranoside
-
vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0
30 - 78
phenyl beta-D-glucoside
0.81
Salicin
-
pH 6.5, 50°C
additional information
additional information
-
substrate inhibition kinetics of wild-type and mutant enzymes, overview
-
0.15
1-deoxynojirimycin
-
40°C, pH 7.0
0.167
1-deoxynojirimycin
-
-
0.29
1-deoxynojirimycin
wild-type, pH 6.0, 20°C
0.058
3,8-dibutylamino-7,8-dideoxy-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.223
3,8-dibutylamino-7,8-dideoxy-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.025
7,8-dideoxy-3,8-didodecylamino-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.367
7,8-dideoxy-3,8-didodecylamino-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.012
7,8-dideoxy-3,8-dioctylamino-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.441
7,8-dideoxy-3,8-dioctylamino-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.0096
7,8-dideoxy-3-hydroxy-8-octylamino-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.484
7,8-dideoxy-3-hydroxy-8-octylamino-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.0029
7,8-dideoxy-8-dodecylamino-3-hydroxy-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.056
7,8-dideoxy-8-dodecylamino-3-hydroxy-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.051
8-butylamino-7,8-dideoxy-3-hydroxy-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.32
8-butylamino-7,8-dideoxy-3-hydroxy-5-octylimino-6-oxa-3-epi-australine
-
pH and temperature not specified in the publication
0.29
beta-D-glucose
-
60°C, pH 4.5
2 - 5
beta-D-glucose
-
50°C, pH 6.0
12
beta-D-glucose
-
50°C, pH 6.0
200
beta-D-glucose
-
30°C, pH 6.0
0.52
cellobiose
-
pH 6.5, 50°C
1.1
cellobiose
-
pH 4.5, 25°C, recombinant mutant W49D
1.2
cellobiose
-
pH 4.5, 25°C, recombinant mutant W49N
1.2
cellobiose
mutant E194A, 30°C, pH 6.0
1.3
cellobiose
-
pH 4.5, 25°C, recombinant mutant W49G
1.4
cellobiose
-
pH 4.5, 25°C, recombinant mutant W49A
1.6
cellobiose
-
pH 4.5, 25°C, recombinant mutants W49N and W49G
1.8
cellobiose
-
pH 4.5, 25°C, recombinant wild-type enzyme and mutant W49A
1.9
cellobiose
-
pH 4.5, 25°C, recombinant mutant W49D
2.8
cellobiose
Thermochaetoides thermophila
-
-
2.8
cellobiose
-
vs. p-nitrophenyl-beta-D-glucopyranoside, 30°C, pH 6.0
2.9
cellobiose
wild-type, 30°C, pH 6.0
3.3
cellobiose
-
25°C, pH 4.5
5.2
cellobiose
mutant M453A, 30°C, pH 6.0
13.7
cellobiose
mutant E190Q, 30°C, pH 6.0
17
cellobiose
mutant E190A, 30°C, pH 6.0
21
cellobiose
wild-type, pH 6.0, 20°C
30
cellobiose
mutant K201F, 30°C, pH 6.0
81
cellobiose
mutant K201A, 30°C, pH 6.0
0.2
cellopentaose
mutant E194A, 30°C, pH 6.0
0.25
cellopentaose
wild-type, 30°C, pH 6.0
0.26
cellopentaose
mutant M453A, 30°C, pH 6.0
0.3
cellopentaose
mutant K201F, 30°C, pH 6.0
0.56
cellopentaose
-
vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0
2
cellopentaose
mutant E190A, 30°C, pH 6.0
2.3
cellopentaose
mutant K201A, 30°C, pH 6.0
16.2
cellopentaose
mutant E190Q, 30°C, pH 6.0
0.14
cellotetraose
mutant E194A, 30°C, pH 6.0
0.2
cellotetraose
wild-type, 30°C, pH 6.0
0.21
cellotetraose
mutant M453A, 30°C, pH 6.0
0.25
cellotetraose
mutant K201F, 30°C, pH 6.0
0.29
cellotetraose
-
vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0
0.54
cellotetraose
mutant K201A, 30°C, pH 6.0
2.4
cellotetraose
mutant E190A, 30°C, pH 6.0
19.6
cellotetraose
mutant E190Q, 30°C, pH 6.0
0.04
cellotriose
mutant K201F, 30°C, pH 6.0
0.07
cellotriose
mutant E194A, 30°C, pH 6.0
0.09
cellotriose
mutant M453A, 30°C, pH 6.0
0.27
cellotriose
wild-type, 30°C, pH 6.0
0.4
cellotriose
-
25°C, pH 4.5
0.41
cellotriose
mutant K201A, 30°C, pH 6.0
0.85
cellotriose
-
vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0
2.3
cellotriose
mutant E190A, 30°C, pH 6.0
8.6
cellotriose
mutant E190Q, 30°C, pH 6.0
0.0083
D-delta-gluconolactone
-
60°C, pH 4.5
0.0083
D-delta-gluconolactone
-
pH 5, 50°C
370
D-galactose
-
versus 2-nitrophenyl-beta-D-galactopyranoside, pH 6.5, 40°C
670
D-galactose
-
25°C, pH 4.5
0.016
D-glucono-1,5-lactone
-
pH 5.5, 37°C
0.1
D-glucono-1,5-lactone
-
pH 4.5, 60°
0.11
D-glucono-1,5-lactone
-
pH 6, 30°C
0.49
D-glucono-1,5-lactone
-
pH 6.2, 50°C
0.012
D-glucono-delta-lactone
recombinant enzyme, using cellobiose as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C
0.02
D-glucono-delta-lactone
-
pH 5.0, 50°C
0.03
D-glucono-delta-lactone
-
pH 4.5, 50°C
0.1
D-glucono-delta-lactone
recombinant enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C
5
D-glucono-delta-lactone
-
40°C, pH 5.0
0.008
D-gluconolactone
-
pH 5.0, 50°C
0.008
D-gluconolactone
-
pH 5.4, 50°C
0.2
D-gluconolactone
-
pH 4.5, 25°C, recombinant mutant W49A
0.4
D-gluconolactone
-
25°C, pH 4.5
0.4
D-gluconolactone
-
pH 4.5, 25°C, recombinant wild-type enzyme
0.24
D-glucose
-
40°C, pH 6.0
0.254
D-glucose
pH 5.0, 72°C
0.254
D-glucose
-
pH and temperature not specified in the publication
0.29
D-glucose
-
40°C, pH 6.0
0.29
D-glucose
-
pH 5, 50°C
0.35
D-glucose
-
pH 5.0, 50°C
0.35
D-glucose
-
pH and temperature not specified in the publication
0.41
D-glucose
-
pH 7.3, 40°C, versus substrate 4-nitrophenyl beta-D-glucopyranoside
0.5
D-glucose
-
pH 5.4, 50°C
0.51
D-glucose
-
at pH 5.0 and 40°C
0.55
D-glucose
-
pH 6.5, 50°C
0.6
D-glucose
-
pH and temperature not specified in the publication
0.79
D-glucose
-
pH 5.0, 50°C
0.79
D-glucose
-
pH and temperature not specified in the publication
0.93
D-glucose
-
at pH 5.0 and 40°C
1.1
D-glucose
recombinant enzyme, using cellobiose as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C
1.4
D-glucose
-
pH 5.0, 50°C
1.5
D-glucose
-
pH and temperature not specified in the publication
1.5
D-glucose
-
at pH 5.0 and 60°C
1.7
D-glucose
-
pH and temperature not specified in the publication
1.7
D-glucose
-
pH 4.5, 60°
2 - 5
D-glucose
pH 5.5, 60°C, with 4-nitrophenyl beta-D-glucopyranoside as substrate, wild-type enzyme
2.3
D-glucose
recombinant enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C
2.7
D-glucose
-
using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM citrate buffer, pH 4.8, at 50°C
2.73
D-glucose
-
at pH 5.0 and 40°C
2.9
D-glucose
-
pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside
3
D-glucose
-
25°C, pH 4.5
3
D-glucose
-
pH 4.5, 25°C, recombinant wild-type enzyme
3.25
D-glucose
-
using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM citrate buffer, pH 4.8, at 50°C
3.27
D-glucose
-
pH 5.0, 50°C
3.4
D-glucose
-
30°C, pH 5.0
3.4
D-glucose
-
using cellobiose as substrate, in 50 mM citrate buffer, pH 4.8, at 50°C
3.5
D-glucose
-
pH 5.0, 42°C
3.7
D-glucose
-
30°C, pH 5.0
3.8
D-glucose
wild-type, pH 6.0, 20°C
5.8
D-glucose
-
30°C, pH 4.6
6.4
D-glucose
-
pH 5.5, 37°C
8.1
D-glucose
-
30°C, pH 5.0
8.3
D-glucose
EU918770
substrate cellobiose, pH 5.0, 55°C
9.2
D-glucose
-
30°C, 5.0
11.02
D-glucose
-
40°C, pH 5.0
13.6
D-glucose
-
pH and temperature not specified in the publication
17.6
D-glucose
EU918770
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.0, 55°C
20
D-glucose
pH 6.0, 70°C
30.8
D-glucose
-
pH 4.0, 30°C
37.1
D-glucose
pH 5.5, 55°C, with 4-nitrophenyl beta-D-glucopyranoside as substrate
39.5
D-glucose
-
pH and temperature not specified in the publication
55.2
D-glucose
pH 6.0, 50°C, with 4-nitrophenyl beta-D-glucopyranoside as substrat, wild-type enzyme
66
D-glucose
-
versus 2-nitrophenyl-beta-D-galactopyranoside, pH 6.5, 40°C
73
D-glucose
-
pH 6.2, 50°C
75
D-glucose
-
pH 4.5, 50°C
84
D-glucose
-
40°C, pH 6.0, vs. genistein-7-O-beta-D-glucopyranoside
150
D-glucose
pH 5.0, 85°C
300
D-glucose
pH 5.0, 50°C, recombinant enzyme
439
D-glucose
-
40°C, pH 5.0
518
D-glucose
H0HC94
mutant V176A, pH not specified in the publication, temperature not specified in the publication
558
D-glucose
H0HC94
mutant L178E, pH not specified in the publication, temperature not specified in the publication
558
D-glucose
H0HC94
mutant W127F, pH not specified in the publication, temperature not specified in the publication
666
D-glucose
H0HC94
mutant L178A, pH not specified in the publication, temperature not specified in the publication
686
D-glucose
H0HC94
wild-type, pH not specified in the publication, temperature not specified in the publication
1000
D-glucose
pH 6.5, 40°C
23.7
D-mannose
-
pH 5.0, 50°C
54
D-mannose
-
pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside
260
D-mannose
-
25°C, pH 4.5
42.7
D-xylose
-
pH 5.0, 50°C
200
D-xylose
pH 5.0, 85°C
340
D-xylose
-
25°C, pH 4.5
0.021
delta-gluconolactone
-
in 50 mM sodium acetate, pH 5.5, at 37°C
0.03
delta-gluconolactone
-
vs. p-nitrophenyl-beta-D-glucopyranoside,30°C, pH 6.0
2.2
delta-gluconolactone
hydrolysis of p-nitrophenyl-beta-D-glucopyranoside
1.8
gentiobiose
-
pH 4.5, 25°C, recombinant mutant W49A
2.8
gentiobiose
wild-type, pH 6.0, 20°C
4.7
gentiobiose
-
25°C, pH 4.5
4.8
gentiobiose
-
pH 4.5, 25°C, recombinant wild-type enzyme
0.0005
glucono-delta-lactone
-
40°C, pH 6.0
0.0006
glucono-delta-lactone
-
40°C, pH 6.0
0.67
glucono-delta-lactone
-
40°C, pH 7.0
23
glucono-delta-lactone
-
40°C, pH 6.0, vs. genistein-7-O-beta-D-glucopyranoside
0.038
gluconolactone
-
50°C, pH 6.0
0.07
gluconolactone
-
pH 5.0, 40°C, versus 4-nitrophenyl beta-D-glucopyranoside
0.12
gluconolactone
-
50°C, pH 6.0
2
gluconolactone
with para-nitrophenyl-beta-D-glucopyranoside as substrate
2.2
gluconolactone
with ortho-nitrophenyl-beta-D-glucopyranoside as substrate
4.7
gluconolactone
with 4-methylumbelliferyl-beta-D-glucopyranoside as substrate
0.7
heptyl beta-D-glucoside
wild-type, 30°C, pH 6.0
1.12
heptyl beta-D-glucoside
mutant K201F, 30°C, pH 6.0
2.6
heptyl beta-D-glucoside
mutant M453A, 30°C, pH 6.0
3.6
heptyl beta-D-glucoside
mutant E194A, 30°C, pH 6.0
4.2
heptyl beta-D-glucoside
mutant K201A, 30°C, pH 6.0
21
heptyl beta-D-glucoside
mutant E190A, 30°C, pH 6.0
21.8
heptyl beta-D-glucoside
mutant E190Q, 30°C, pH 6.0
1.57
hexyl beta-D-glucoside
wild-type, 30°C, pH 6.0
3.3
hexyl beta-D-glucoside
mutant K201F, 30°C, pH 6.0
5.6
hexyl beta-D-glucoside
mutant M453A, 30°C, pH 6.0
8
hexyl beta-D-glucoside
mutant E194A, 30°C, pH 6.0
10
hexyl beta-D-glucoside
mutant K201A, 30°C, pH 6.0
32
hexyl beta-D-glucoside
mutant E190A, 30°C, pH 6.0
44.9
hexyl beta-D-glucoside
mutant E190Q, 30°C, pH 6.0
5.1
methyl beta-D-glucoside
wild-type, 30°C, pH 6.0
8
methyl beta-D-glucoside
mutant E190A, 30°C, pH 6.0
10
methyl beta-D-glucoside
mutant K201F, 30°C, pH 6.0
10.1
methyl beta-D-glucoside
mutant E194A, 30°C, pH 6.0
10.2
methyl beta-D-glucoside
mutant M453A, 30°C, pH 6.0
12.1
methyl beta-D-glucoside
mutant E190Q, 30°C, pH 6.0
15.6
methyl beta-D-glucoside
mutant K201A, 30°C, pH 6.0
0.13
nonyl beta-D-glucoside
mutant K201F, 30°C, pH 6.0
0.22
nonyl beta-D-glucoside
wild-type, 30°C, pH 6.0
0.5
nonyl beta-D-glucoside
mutant M453A, 30°C, pH 6.0
0.83
nonyl beta-D-glucoside
mutant E194A, 30°C, pH 6.0
1.16
nonyl beta-D-glucoside
mutant K201A, 30°C, pH 6.0
5.7
nonyl beta-D-glucoside
mutant E190Q, 30°C, pH 6.0
5.9
nonyl beta-D-glucoside
mutant E190A, 30°C, pH 6.0
0.42
octyl beta-D-glucoside
mutant K201F, 30°C, pH 6.0
0.44
octyl beta-D-glucoside
wild-type, 30°C, pH 6.0
1.3
octyl beta-D-glucoside
mutant M453A, 30°C, pH 6.0
1.49
octyl beta-D-glucoside
mutant E194A, 30°C, pH 6.0
2.52
octyl beta-D-glucoside
mutant K201A, 30°C, pH 6.0
10.3
octyl beta-D-glucoside
mutant E190A, 30°C, pH 6.0
11
octyl beta-D-glucoside
mutant E190Q, 30°C, pH 6.0
3.1
octyl-beta-D-glucopyranoside
-
vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0
4
octyl-beta-D-glucopyranoside
-
vs. 4-methylumbelliferyl-beta-D-glucopyranoside, 30°C, pH 6.0
30
phenyl beta-D-glucoside
mutant E190A, 30°C, pH 6.0
36
phenyl beta-D-glucoside
mutant E194A, 30°C, pH 6.0
48
phenyl beta-D-glucoside
mutant K201F, 30°C, pH 6.0
49
phenyl beta-D-glucoside
wild-type, 30°C, pH 6.0
52
phenyl beta-D-glucoside
mutant M453A, 30°C, pH 6.0
74
phenyl beta-D-glucoside
mutant K201A, 30°C, pH 6.0
78
phenyl beta-D-glucoside
mutant E190Q, 30°C, pH 6.0