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3.2.1.21: beta-glucosidase

This is an abbreviated version!
For detailed information about beta-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.21

Reaction

celloheptaose
+ 6 H2O = 7 beta-D-glucose

Synonyms

1,4-beta-glucosidase, 3BGlu6, 4BGlu12, A1, amygdalase, amygdalin hydrolase, amygdalinase, arbutinase, aryl beta-glucosidase, aryl-beta-D-glucosidase, aryl-beta-glucosidase, AT5A_15831, beta-1,6-glucosidase, beta-D-glucosidase, beta-D-glucosidase F1, beta-D-glucoside glucohydrolase, beta-Glcase, beta-glu, beta-Glu x, beta-glu1, beta-glu2, beta-glucosidase, beta-glucosidase 1, beta-glucosidase 6, beta-glucosidase B, beta-glucosidase C, beta-glucosidase I, beta-glucosidase II, beta-glucoside hydrolase, beta-GLYPI, betaG, betagly, BG, BG1, BGA, Bgl, BGL1, BGL1A, BGL1B, Bgl2, Bgl3, bgl3a, Bgl3B, BGL50, BglA, BglB, BglC, BglD5, Bglhi, BglHi2, BglI, BglII, BglP, BGLPf, BglT, BGLU, BGlu1, BGLU21, BGLU22, BGLU23, Bglu3B, bgpA, BGs, BGX1, bifunctional beta-glucosidase/xylosidase, bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase, Cba3, CBG, CC1G_08724, Cel3A, Cel3b, CelB, cellobiase, CGHII, cyanogenic beta-glucosidase, dalcochinase, Dhr1, dhurrinase-1, DV-BG, elaterase, emulsin, esculinase, F1, family-1 beta-glycosidase, FPG, G-II, GBA, GBA2, GBA3, gentiobiase, GH1 beta-glucosidase, GH3 beta-glucosidase, Gh3-4, GI, GII, ginsenoside-hydrolyzing beta-D-glucosidase, Gl-2, Gl-3, Glu1, Glu1b, Glu2, GLU3, GLU4, hCBG, HGT-BG, HiBgl3A, HiBgl3B, HiBgl3C, Hore_15280, ICHG, iridoid beta-glucoside, isoflavone conjugate-hydrolyzing beta-glucosidase, J1, Klotho-related protein, KLrP, KNOUC202beta-gly, lactase, limarase, Lin1840, linamarase, linustatinase, LjBGD2, LjBGD4, LjBGD7, MeBglD2, More, Novozyme 188, oligofurostanoside-specific beta-glucosidase, OsTAGG, p-nitrophenyl beta-glucosidase, PF0073, PGI, PGII, piceid-beta-D-glucosidase, PRGH1, primeverosidase, prunasin hydrolase, reCBG, RuBGX1, SA-bglu, salicilinase, salicinase, SbDhr1, Sde1394, SP188, SSO3039, SYNbglA, T-cell inhibitor, T1, Td2F2, Tnap_0656, Tpbgl, TpBGL1, TpBGL3, Tpebgl3, Tpen_1494, tuberonic acid glucoside (TAG)-hydrolyzing beta-glucosidase, tuberonic acid glucoside beta-glucosidase, Umbgl3B, vicianase, ZM-p60.1, ZmGlu1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.21 beta-glucosidase

Cloned

Cloned on EC 3.2.1.21 - beta-glucosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
bgl operon, gene bglB, contruction of a genomic library, DNA and amino acid sequence determination and analysis, determination of bgl operon structure and organization, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) as His-tagged proteins, subcloning in Escherichia coli strain DH5alpha
cDNA expressed in insect cells, Baculovirus expression system
cDNA is expressed in insect cells, Baculovirus expression system
cloned in frame with the Saccharomyces cerecisiae a-factor secretory signal and expressed in Pichia pastoris strain X-33
cloned into the pET-28b vector. His-tagged MeBglD2 is expressed in Escherichia coli BL21(DE3) cells
DNA and amino acid sequence determination and anaylsis, expression analysis by semi-quantitative RT-PCR, stable expression of the 35S-GmICHG:GFP fusion gene in Arabidopsis thaliana T87 cells using an Agrobacterium tumefaciens GV3101(pMP90) transformation system, phylogenetic analysis
enzyme with an N-terminal truncation following the propeptide and eight histidine residues enabling its purification by immobilized metal-ion affnity chromatography, functional, methanol-inducible enzyme expression in Pichia pastoris strain GS115 and Saccharomyces cerevisiae strains DY150 and DBY746, addition of 0.5% casamino acids to the culture medium stabilizes the pH of the culture and increaseds the protein yield by 3-5folds, insertion of a polyhistidine-tag either after the N-terminal alpha-factor signal sequence or at the C-terminus fails to assist in purification by immobilized metal-ion affnity chromatography due to post-translational processing at both termini, expression in Escherichia coli strain BL21(DE3) as insoluble and inactive protein, which can be made soluble by co-expression with bacterial chaperonin GroESL
-
expressed in Aspergillus oryzae
expressed in Escherichia coli
expressed in Escherichia coli as a GST-tagged fusion protein
-
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli B834 (DE3) cells
expressed in Escherichia coli BL21
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLyS cells
expressed in Escherichia coli Origami (DE3) cells
expressed in Escherichia coli Origami B (DE3) cells
expressed in Escherichia coli under regulation of a T7/lac promoter. On induction with isopropyl-beta-D-1-thiogalactopyranoside, the enzyme expresses at 40% of the cell protein producing 238 mg/liter culture. With increase in culture cell density to A600 12 in auto-inducing M9NG medium, beta-glucosidase production increases 3fold. Approximately 70% of the expressed enzyme is in a soluble form, while the rest is in an insoluble fraction of the cell lysate
Halalkalibacterium halodurans
-
expressed in Pichia pastoris
expressed in Pichia pastoris strain KM71H
-
expressed in Pichia pastoris strain X33
-
expressed in Pichia pastoris strain Y11430
-
expressed in Pseudomonas putida KT2440
expressed in Saccharomyces cerevisiae
-
expressed in Trichoderma reesei
expressed in Trichoderma reesei strain QM9414
-
expressed on Corynebacterium glutamicum strain CM cell surface
-
expression as fusion protein with alpha-agglutinin on surface of Saccharomyces cerevisiae cells
expression in Escherichia coli
expression in Escherichia coli strain BL21 Codon-Plus
expression in Escherichia coli strain JM109
-
expression in Hypocrea jecorina
expression in lactic-acid producing Saccharomyces cerevisiae strain, expression on cell surface by fusing the mature protein to the C-terminal half region of alpha-agglutinin
expression in Pichia pastoris
expression in Saccharomyces cerevisiae
expression in Streptomyces lividans
-
expression of His-tagged isozyme BGL1A in Escherichia coli
expression of His-tagged wild-type and mutant enzymes in Escherichia coli
expression of His-tagged wild-type Bgl3B in Escherichia coli strain Tuner (DE3), and of His-tagged selenomethionine-labeled enzyme in Escherichia coli strain B834 (DE3)
-
expression of isozyme BglB in Escherichia coli
expression of N-terminally His6-tagged cytosolic isozyme in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
-
expression of wild-type and mutant BglC in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha, display of the enzyme on the cell surface of Saccharomyces cerevisiae
-
expression of wild-type and mutant enzymes in Pichia pastoris
expression of wild-type and mutant hybrid of BGL1-CBD2 domain with cellobiohydrolase CBHII in in Saccharomyces cerevisiae strain CENPK42 under the control of phosphoglycerate-kinase-1 promoter PGK1P and terminator PGK1T and yeast mating pheromone alpha-factor secretion signal MFalpha1S, subcloning in Escherichia coli
-
exprression in Escherichia coli
gene bgl2, DNA and amino acid sequence determination and anaylsis, expression of a His-tagged cDNA fragment excluding the potential signal sequence in Pichia pastoris strain KM71H
gene bglA, DNA and amino acid sequence determination and analysis, cDNA library construction, expression in Escherichi coli
gene bglA, expression of the His-tagged enzyme in Escherichia coli
-
gene bglA, recombinant expression in Escherichia coli strain BL21 Codon-Plus
gene bglb, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His-tagged wild-type and chimeric mutant enzymes in Escherichia coli
-
gene bglu1, expression in in Escherichia coli with N-terminal thioredoxin and hexahistidine tags
gene cba3, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of the extracellular His6-tagged enzyme in Escherichia coli strain JM101, transformants expressing recombinant Cba3 grow readily in minimal media using cellobiose as the sole carbon source
gene cbg1, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His-tagged wild-type and chimeric mutant enzymes in Escherichia coli
-
gene cel3a, DNA and amino acid sequence determination and analysis, subcloning and expression in Escherichia coli, expression in Trichoderma reesei
gene celG encoded in the celEFG operon, construction of a cosmid library and cloning of the celEFG operon, genetic organizatin, DNA and amino acid sequence determination and analysis, phylogenetic tree, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene identification in Escherichia coli
heterogeneous expression in Pichia pastoris strain GS115
in Arabidopsis thaliana
-
isozyme BGL1A, DNA and amino acid sequence determination and analysis, transcription analysis, expression of C-terminally His-tagged enzyme in Escherichia coli
isozyme BGL1B, DNA and amino acid sequence determination and analysis, transcription analysis, expression of C-terminally His-tagged enzyme in Escherichia coli
isozyme TaGlu1a, DNA and amino acid sequence determination and analysis, isozyme expression profile, expression of wild-type and mutant isozyme TaGlu1a in Escherichia coli
isozyme TaGlu1b, DNA and amino acid sequence determination and analysis, isozyme expression profile, expression in Escherichia coli
isozyme TaGlu1c, DNA and amino acid sequence determination and analysis, isozyme expression profile, expression in Escherichia coli
methanol-inducible expression of wild-type and mutant enzymes in Pichia pastoris strain X-33
-
native and mutated cDNA sequences cloned into vector pPICZalphaB and expressed in Pichia pastoris
overexpressed in Escherichia coli
-
overexpression in Escherichia coli
overexpression of Dhr1 and mutant E189D in Escherichia coli as His-tagged proteins
-
overexpression of His-tagged enzyme in Escherichia coli strain JM109
overexpression of His-tagged Glu1b in Escherichia coli as soluble protein
-
single gene, genomic organization, DNA and amino acid sequence determination and analysis, expression in Spodoptera frugiperda Sf9 cells using the baculovirus infection system
wild type and mutant enzymes Q77R, D206N, D206Q, E207S and E399S are expressed in Escherichia coli