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1-Pentanol
-
1.6fold increase of activity
1-propanol
-
10% stimulation at 4%, inhibition above
2-butanol
3fold activation
2-Methyl-1-pentanol
addition of 20% v/v n-octanol results in a higher activity of 4-nitrophenyl-beta-D-glucopyranoside hydrolysis, and activation of transglycosylation activity, overview
2-methyl-2-pentanol
addition of 20% v/v n-octanol results in a higher activity of 4-nitrophenyl-beta-D-glucopyranoside hydrolysis, and activation of transglycosylation activity, overview
2-propanol
9fold activation
3-methyl-1-pentanol
addition of 20% v/v n-octanol results in an over 40fold higher activity of 4-nitrophenyl-beta-D-glucopyranoside hydrolysis, and activation of transglycosylation activity, overview
4-chloromercuribenzoate
-
9% activation at 1 mM
4-hydroxybenzoic acid
-
109.7% activity at 2.5 mM with cellobioside as substrate
4-Methyl-1-pentanol
addition of 20% v/v n-octanol results in a higher activity of 4-nitrophenyl-beta-D-glucopyranoside hydrolysis, and activation of transglycosylation activity, overview
acetone
hydrolysis activity is enhanced in the presence of non-acceptor type organic solvents such as hexane, cyclohexane, diethylether, and acetone
acetonitrile
-
20%, 176% activation
allyl alcohol
6.7fold activation
beta-D-glucose
-
cloned enzyme is activated 2-3fold at 0.1-0.3 M
Ca2+
-
15% activation at 1 mM
CaCl2
-
40 mmol/l, 233.33% relative enzyme activity in midgut, 193.31% relative enzyme activity in salivary glands
Cyclohexane
hydrolysis activity is enhanced in the presence of non-acceptor type organic solvents such as hexane, cyclohexane, diethyl ether, and acetone
diethylether
hydrolysis activity is enhanced in the presence of non-acceptor type organic solvents such as hexane, cyclohexane, diethylether, and acetone
dimethyl sulfoxide
-
20%, 92% activation
Dimethylsulfoxide
-
2 mM, 23% activation
dithioerythritol
-
1 mM, slight activation
ethyl acetate
-
20%, 171% activation
Fe3+
-
10 mM, 115% of initial activity
Gaucher spleen heat-stable factor
-
15fold increase for hydrolysis of 17beta-estradiol-17'-beta-D-glucoside
-
GSH
-
slight activation at 1 mM
hexane
hydrolysis activity is enhanced in the presence of non-acceptor type organic solvents such as hexane, cyclohexane, diethyl ether, and acetone
Inositol
-
constant enzyme activation, values of relative activation about 40%
Isopropanol
-
4fold activation by preincubation with 80%, v/v
jacalin-related lectin
-
isoform Pyk10 is localized in ER bodies and has beta-D-glucosidase and beta-D-fucosidase activities. Jacalin-related lectins regulate the size of the active Pyk10 complexes, with two types of lectins working antagonistically
-
Lubrol-WX
-
28% enhanced activity at 1%, w/v
MgCl2
-
40 mmol/l, 200% relative enzyme activity in midgut, 169.49% relative enzyme activity in salivary glands
n-butanol
14fold activation
n-Heptanol
addition of 20% v/v n-octanol results in an over 40fold higher activity of 4-nitrophenyl-beta-D-glucopyranoside hydrolysis, and activation of transglycosylation activity, overview
n-Hexanol
addition of 20% v/v n-octanol results in an over 40fold higher activity of 4-nitrophenyl-beta-D-glucopyranoside hydrolysis, and activation of transglycosylation activity, overview
n-Octanol
addition of 20% v/v n-octanol results in a 54fold higher activity of 4-nitrophenyl-beta-D-glucopyranoside hydrolysis, and activation of transglycosylation activity, overview
n-Pentanol
23fold activation
n-Propanol
9.3fold activation
NaCl
-
40 mmol/l, 198.57% relative enzyme activity in midgut, 163.63% relative enzyme activity in salivary glands
p-coumaric acid
-
118% activity at 2.5 mM with cellobioside as substrate
protein PBPI
binding protein, interacts with beta-glucosidases and activates them
-
protein PBPII
binding protein, interacts with beta-glucosidases and activates them
-
SDS
1 mg/ml, native enzyme is activated to 118.3% of control, recombinant enzyme is activated to 115.6% of control, beta-glucosidase activity
syringic acid
-
111.4% activity at 2.5 mM with cellobioside as substrate
Tween 20
5.5fold activation
Tween 80
3.6fold activation
Tween-20
-
53% enhanced activity at 1%, w/v
vanillic acid
-
125.1% activity at 2.5 mM with cellobioside as substrate
Vanillin
-
118% activity at 2.5 mM with cellobioside as substrate
Zn2+
presence of 2 mM Zn2+ increases the relative activity of the immobilized enzyme to 192% and 199% with cellobiose and 4-nitrophenyl-beta-D-glucopyranoside as substrates
1-butanol
-
2.2fold increase of activity at 0.5 M
1-butanol
-
1.6fold increase of activity
2-mercaptoethanol
Melanocarpus sp.
-
-
2-mercaptoethanol
-
2fold enhanced activity
2-mercaptoethanol
-
slight activation at 1 mM
2-mercaptoethanol
-
129.9% relative activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 1-2 mM
2-mercaptoethanol
thermophilic anaerobic bacterium
-
30% stimulation at 1 mM
2-mercaptoethanol
-
500 mM, 2fold activation, inhibition above
beta-mercaptoethanol
-
1 mM, slight activation
beta-mercaptoethanol
-
the purified enzyme shows 152.9% relative activity at 1% (v/v)
Cysteine hydrochloride
-
slight activation at 1 mM
Cysteine hydrochloride
thermophilic anaerobic bacterium
-
slight activation at 5 mM
D-galactose
-
50 mM, 123% of initial activity
D-glucose
1.8fold stimulation at 50 mM
D-glucose
-
the enzyme is activated by up to 250 mM D-glucose
D-glucose
-
50 mM, 204% of initial activity, activating constant 36.7 mM
D-glucose
at concentrations lower than 400 mM
D-glucose
-
D-glucose (1% w/v) in the reaction medium stimulates beta-glucosidase activity by about 2fold in crude extracts from mycelia grown in sugarcane bagasse
D-xylose
1.9fold stimulation at 50 mM
D-xylose
-
50 mM, 191% of initial activity, activating constant 43.2 mM
D-xylose
-
xylose (1% w/v) in the reaction medium stimulates beta-glucosidase activity by about 2fold in crude extracts from mycelia grown in sugarcane bagasse
dithiothreitol
-
1 mM, slight activation
dithiothreitol
-
slight activation at 1 mM
dithiothreitol
-
the purified enzyme shows 108.4% relative activity at 20 mM
dithiothreitol
-
stimulation at 10 mM
dithiothreitol
thermophilic anaerobic bacterium
-
slight activation at 1 mM
DMSO
10% v/v, 106% of initial activity, 20% v/v, 112.6% of initial activity
DMSO
10% (v/v), native enzyme is activated to 102.3% of control, recombinant enzyme is activated to 106.4% of control, beta-glucosidase activity
DTT
Melanocarpus sp.
-
-
EDTA
-
slight activation
EDTA
-
strong activation of both PGI and PGII at 10 mM
EDTA
-
10% activation at 1 mM
EDTA
1 mM, native enzyme is activated to 110.7% of control, recombinant enzyme is activated to 108.2% of control, beta-glucosidase activity
EDTA
thermophilic anaerobic bacterium
-
slight activation at 5 mM
ethanol
-
30% stimulation at 15%, v/v , 15% stimulation at 20%, v/v
ethanol
-
activation depends on the yeast strain, e.g. strain Y8 shows 5fold increased activity at 14% ethanol in wine solution, overview
ethanol
-
activation up to 3 M
ethanol
-
activation up to 3 mM, above inactivation
ethanol
-
in the presence of 16% (v/v) ethanol the enzyme activity is increased more than 2fold
ethanol
Melanocarpus sp.
-
-
ethanol
-
up to 10% v/v, increase in activity to 120%
ethanol
-
slight stimulation at low concentrations
ethanol
-
11% stimulation at 0.75%, v/v
ethanol
10% v/v, 180% of initial activity, 20% v/v, 70% of initial activity
ethanol
10%, 127% of initial activity, 50%, 0.6% of initial activity
ethanol
-
at up to 15% v/v
ethanol
-
15% (v/v) ethanol increases the enzyme activity
ethanol
-
strain AL41 shows increased activity at 14% ethanol in wine solution
ethanol
-
30%, 40% activation
ethanol
high enzyme activation at 20-30%
ethanol
10%, 1.1fold activation
ethanol
-
20%, 197% activation
ethanol
-
10% v/v, 210% of initial activity
glycerol
10%, 121% of initial activity
glycerol
thermophilic anaerobic bacterium
-
25% stimulation at 10%, w/v
L-cysteine
-
2.5fold enhanced activity at 10 mM
L-cysteine
-
50% increase of activity
methanol
-
1.4fold increase of activity at 1 M
methanol
-
in the presence of 16% (v/v) methanol the enzyme activity is increased more than 2fold
methanol
Melanocarpus sp.
-
-
methanol
-
slight stimulation at low concentrations
methanol
10%, 121% of initial activity, 50%, 1.3% of initial activity
methanol
-
at up to 15% v/v
methanol
-
15% stimulation at 4%, inhibition above
methanol
-
30%, 30% activation
methanol
high enzyme activation at 20-30%
methanol
10%, 1.1fold activation
methanol
-
20%, 168% activation
propan-2-ol
10%, 107% of initial activity, 50%, 0.2% of initial activity
propan-2-ol
-
concentrations below 50%, activation
Triton X-100
10 mg/ml, native enzyme is activated to 113.4% of control, recombinant enzyme is activated to 111.7% of control, beta-glucosidase activity
Triton X-100
4.5fold activation
additional information
-
poor activation by glycerol, dimethyl sulfoxide, and dithiothreitol
-
additional information
-
high concentrations (up to 0.2 M) of fructose, malate, lactate, mannitol and sorbitol have little or no effect on the enzyme activity
-
additional information
-
mannose, fructose, ribose, galactose, sorbose, arabinose, maltose and sucrose are without effect
-
additional information
the enzyme is induced by growth on rice bran hemicellulose, and slightly by pectin, xylan, and glucose, no effect by EDTA, L-fucose, G-glucuronic acid, D-mannose, L-rhamnose, and D-xylose
-
additional information
-
no activation by microwave (300 W)
-
additional information
at -20 to 40 °C no significant enzymatic activity is detected in the absence of microwave irradiation. However, a greater than 4 orders of magnitude increase in enzymatic activity of Pfu CelB is achieved with 300 W of microwave irradiation
-
additional information
-
the enzyme is not affected by glycerol, EDTA, acetic acid, SDS, Triton X-100, D-glucose, and ethanol
-
additional information
-
the addition of vitamins such as thiamin hydrochloride, folic acid, pyridoxine hydrochloride, and riboflavin increases the enzyme activity. A 1.6fold increase in enzyme activity is observed when the culture medium is supplemented with 20 mg/l of inositol
-
additional information
the hydrophobic linker region in BGLII is required for activation by organic solvents
-
additional information
-
the hydrophobic linker region in BGLII is required for activation by organic solvents
-
additional information
the enzyme is unaffected by EDTA and Tween 20
-
additional information
-
the enzyme is unaffected by EDTA and Tween 20
-
3.2.1.21 beta-glucosidase
results (
results (
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