3.2.1.201: exo-chitinase (reducing end)
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For detailed information about exo-chitinase (reducing end), go to the full flat file.
Reaction
Hydrolysis of N,N'-diacetylchitobiose from the reducing end of chitin and chitodextrins =
Synonyms
ChiA, ChiD
ECTree
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General Information
General Information on EC 3.2.1.201 - exo-chitinase (reducing end)
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metabolism
the presence of the two chitin-binding domains significantly enhance the termostability of the catalytic domain
physiological function
KC698970
a tripartite combination of ChiA with ChiB and ChiC exhibits no synergistic effects and produces levels of chitin oligomers similar to that produced by ChiB plus ChiC
physiological function
-
combining the chitinases ChiA, ChiB and ChiC reveals clear synergies for all substrates tested
physiological function
digestion of beta-chitin by ChiA occurs from the reducing end to the nonreducing end. Chitinases ChiA and ChiB show synergistic interactions in simultaneous degradation of beta-chitin, but not of glycol chitin
physiological function
the enzyme has a multidomain structure containing dual catalytic domains and triple chitin-binding domains.The N-terminal catalytic domain mainly hydrolyzes the second glycosidic bond from the nonreducing end of the oligomers, whereas the C-terminal domain randomly hydrolyzes glycosidic bonds other than the first bond from the nonreducing end. Both catalytic domains form diacetylchitobiose as a major end product and possess transglycosylation activity. The N-terminal catalytic domain exclusively liberates diacetylchitobiose, whereas reactions with the C-terminal domain lead to N-acetyl-chitooligosaccharides of various lengths. A synergistic effect is observed when chitin is degraded in the presence of both catalytic domains
physiological function
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digestion of beta-chitin by ChiA occurs from the reducing end to the nonreducing end. Chitinases ChiA and ChiB show synergistic interactions in simultaneous degradation of beta-chitin, but not of glycol chitin
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physiological function
-
a tripartite combination of ChiA with ChiB and ChiC exhibits no synergistic effects and produces levels of chitin oligomers similar to that produced by ChiB plus ChiC
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