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3.2.1.201: exo-chitinase (reducing end)

This is an abbreviated version!
For detailed information about exo-chitinase (reducing end), go to the full flat file.

Reaction

Hydrolysis of N,N'-diacetylchitobiose from the reducing end of chitin and chitodextrins =

Synonyms

ChiA, ChiD

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.201 exo-chitinase (reducing end)

Crystallization

Crystallization on EC 3.2.1.201 - exo-chitinase (reducing end)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ChiA comprises an N-terminal domain, a catalytic (beta/alpha)8 barrel domain and a small (alpha +beta) domain, which is inserted into the (beta/alpha)8 barrel domain. The cocrystallized chito-oligosaccharide is inserted into the catalytic cleft from the reducing end when digested by ChiB
ChiA crystallizes both by salting-in and salting-out in the presence of a variety of precipitating agents in a wide range of concentrations and pH values