3.2.1.201: exo-chitinase (reducing end)
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For detailed information about exo-chitinase (reducing end), go to the full flat file.
Reaction
Hydrolysis of N,N'-diacetylchitobiose from the reducing end of chitin and chitodextrins =
Synonyms
ChiA, ChiD
ECTree
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Crystallization
Crystallization on EC 3.2.1.201 - exo-chitinase (reducing end)
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ChiA comprises an N-terminal domain, a catalytic (beta/alpha)8 barrel domain and a small (alpha +beta) domain, which is inserted into the (beta/alpha)8 barrel domain. The cocrystallized chito-oligosaccharide is inserted into the catalytic cleft from the reducing end when digested by ChiB
ChiA crystallizes both by salting-in and salting-out in the presence of a variety of precipitating agents in a wide range of concentrations and pH values