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3.2.1.20: alpha-glucosidase

This is an abbreviated version!
For detailed information about alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.20

Reaction

maltotetraose
+ 3 H2O = 4 alpha-D-glucose

Synonyms

6-phospho-alpha-glucosidase, AAGR-1, AAGR-2, AAGR-3, AAGR-4, acid alpha-glucosidase, acid alpha-glucoside hydrolase, acid GAA, acid maltase, acidic alpha-glucosidase, AG, AgdB, AGL, AglA, aGlu, alglucosidase alfa, alpha-1,4-glucosidase, alpha-D-glucosidase, alpha-D-glucoside glucohydrolase, alpha-GD, alpha-glucopyranosidase, alpha-glucosid-specific GH4 enzyme, alpha-glucosidase, alpha-glucosidase B, alpha-glucosidase I, alpha-glucosidase II, alpha-glucosidase III, alpha-glucosidase MAL12, alpha-glucosidase MAL32, alpha-glucosidase type I, alpha-glucosidase type IV, alpha-glucoside glucohydrolase, alpha-glucoside hydrolase, alpha-transglucosidase, alphaGlu1, alphaGlu2, alphaGlu3, alphaGluFa, Amy112, CHEI, CWH41, exo-alpha-l, 4-glucosidase, exo-alpha-l,4-glucosidase, GAA, GH13 alpha-glucosidase, GluA, glucoamylase, glucoinvertase, glucosidoinvertase, glucosidosucrase, GlyFa1, GlyFa2, GSJ, HBG III, HBGase I, HBGase II, HBGase III, high pI alpha-glucosidase, intestinal alpha-glucosidase, intestinal maltase, JHGase I, MAL12, MAL2, MAL32, MalA, MalH, maltase, maltase glucoamylase, maltase-glucoamylase, maltodextrin glucosidase, MalZ, MGAM, More, myozyme, NAG, neutral alpha-glucosidase, PalH, Pars_2044, phospho-alpha-glucosidase, R-glucosidase, recombinant human GAA, rhGAA, Saci1160, st2525, Ta0298, TcaAG, TtGluA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.20 alpha-glucosidase

Cloned

Cloned on EC 3.2.1.20 - alpha-glucosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
by screening of the cDNA library and the genomic DNA library, DNA and amino acid sequence determination and analysis
cloning of the maltase gene from strain 727-14C, cloning from a cDNA library and amino acid sequence determination, expression of wild-type enzyme in Escherichia coli strain KP3998, expression of chimeric maltase/isomaltase mutant enzymes in Escherichia coli strain KP3998
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construction of a genomic library, gene mapping, DNA and amino acid sequence determination and analysis, expression in Escherichia coli HB101 and DH5alpha
DNA and amino acid sequence determination and analysis, expression in Escherichia coli
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DNA and amino acid sequence determination and analysis, expression in Escherichia coli, phylogenetic analysis
DNA and amino acid sequence determination and anaylsis
DNA and amino acid sequence determination and anaylsis, semi-quantitative expression analysis, sequence comparison
DNA sequence determination and analysis, genomic organization of the gene cluster, functional overexpression in Escherichia coli JM83
enzyme expression in Lactococcus lactis strain NZ9000, method optimization, overview
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expressed as a fusion protein in Escherichia coli
expressed in Bacillus subtilis ISW1214 cells
expressed in Escherichia coli DH5alpha
expressed in Escherichia coli MC1061 cells
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expressed in Escherichia coli Rosetta cells
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expressed in Mus musculus
expressed in Sulfolobus acidocaldarius. Attempts to obtain a soluble enzyme from Escherichia coli strains are unsuccessful
expression in Chinese hamster ovary K1 cells
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expression in COS-7 cells
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in mammary gland of transgenic mice
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expression in Pichia pastoris
expression of the thermostable, cytosolic enzyme in Escherichia coli strain BL21(DE3) using a microfiltration membrane bioreactor to improve the control of the concentration of key components in the growth of the cells, overview
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expression of wild-type and mutant enzymes in Escherichia coli strain JM109
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functional overexpression in Escherichia coli
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gene AciHBGase II, DNA and amino acid sequence determination and analysis, recombinant expression of the C-terminally His-tagged secretory enzyme in Pichia pastoris strain GS115
gene Agl, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Pichia pastoris
gene agl97, expression of functional His-tagged full-length enzyme in Pichia pastoris, production by high cell-density fermentation
gene aglA, expression analysis and mapping of transcription start sites, the gene is clustered in the genome and shows colinear orientation and a small intergenic space with gene manA encoding alpha-mannosidase, the genes are independently transcribed, both producing leaderless mRNA, phylogenetic tree, overexpression in Escherichia coli strain BL21, subcloning in Escherichia coli strain XL-1 Blue
gene aglA, overexpression of the extracellular enzyme in Emericella nidulans strain JCM10259
gene aglA, recombinant expression in Saccharomyces cerevisiae strain BY474, which lacks maltose, under control of a galactose-inducible promoter. Recombinant yeast cells expressing the aglA gene produce extracellular alpha-glucosidase activity about half of which appeared cell bound whereas the other half is released into the culture medium
gene aglU, recombinant expression from plasmid pKANGL-GCW61 in Pichia pastoris strain GS115, cell surface display, recombinant expression in Escherichia coli TOP10 cells
gene amy112, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Alkalihalophilus pseudofirmus
gene gluA, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene MAL2, genomic library construction, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli as N- and C-terminally His-tagged protein, expression pattern analysis of the gene in potato plants by Northern blot analysis
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gene malA, DNA and amino acid sequence analysis, phylogenetic tree, overexpression of wild-type and selenomethionine-labeled enzyme in Escherichia coli strain NF1830
gene malA, library preparation, DNA and amino acid sequence determination and analysis, genetic structure and organization, sequences 3' to position -33, including a consensus archaeal TATA box, play an essential role in malA expression, detection of malA homologues, overview, phylogenetic analysis, expression of MalA in Escherichia coli revealing differences from the native enzyme in thermostability and electrophoretic behavior, overview
gene malA, overexpression of wild-type and selenomethionine-labeled enzyme in Escherichia coli
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gene palH, palH is present in a cluster of nine genes whose products may facilitate the transport, and metabolism of palatinose in this plant pathogen, phylogenetic analysis, expression of soluble His-tagged wild-type and mutant PalHs in Escherichia coli
gene SsGA, DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3), cytosolic recombinant enzyme
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genes gluA and gluB, BAC library construction, DNA and amino acid sequence determination and analysis, expression analysis, expression of the isozymes in Escherichia coli strain XL 1-Blue cytosol
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genotyping of wild-type and mutant enzymes, cDNA sequencing of the Mgam null mice
mutant enzyme D408G, expression in Escherichia coli BL21(DE3)
N- and C-terminal catalytic subunits, expression of His6-tagged N-terminal subunit ntMGAM in Drosophila S2 cells, expression of His6-tagged C-terminal subunits ctMGAMN2 and ctMGAMN20 in Spodoptera frugiperda Sf9 cells
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overexpression in Escherichia coli DH5 alpha
overexpression of the N-terminal catalytic subunit in Drosophila melanogaster S2 cells and secretion of the recombinant protein from the cells
overproduced in Escherichia coli
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produced in CHO cells (CHO-GAA). rhGAA purified from the milk of transgenic Oryctolagus cuniculus
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recombinant expression of N-terminally His-tagged N-terminal domain in Drosophila melanogaster S2 cells
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