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3.2.1.2: beta-amylase

This is an abbreviated version!
For detailed information about beta-amylase, go to the full flat file.

Word Map on EC 3.2.1.2

Reaction

(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose
+
H2O
=
(alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose
 +
alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose

Synonyms

(1-4)-alpha-D-glucan maltohydrolase, 1,4-alpha-D-glucan malto-hydrolase, 1,4-alpha-D-glucan maltohydrolase, 1-4-alpha-glucan maltohydrolase, alpha-1,4-glucan maltohydrolase, amylase, beta-, ARATH, BAM-1, BAM-2, BAM-3, BAM-5, BAM-6, BAM-7, BAM-8, BAM-9, BAM1, BAM3, BAM4, BCB, beta amylase, beta-amylase, beta-amylase 1, beta-amylase I, beta-amylase1, beta-amylase2, beta-amylase8, BMY, Bmy1, Bmy2, Cs-COR018, CT-BMY, glycogenase, More, PF0870, saccharogen amylase, saccharogenamylase, SBA, Sd1, Sd2H, Sd2L, spoII, TCMA, TR-BAMY, type I beta-amylase, type II beta-amylase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.2 beta-amylase

Purification

Purification on EC 3.2.1.2 - beta-amylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
4 isoforms
-
affinity chromatography, to near homogeneity using Glutathione-Sepharose 4B
-
beta-amylase I and II
-
continuous extraction in a PEG/CaCl2 aqueous two-phase system. The best system for recovering the maize malt enzymes is with low vane rotation and flux rate and high free area of vane
-
E172A, E367A and E172A/E367A mutant enzymes
-
gel filtration
-
isoenzyme 6
-
multiple forms
-
mutant beta-amylases
native beta-amylase from both mature grain and germinated barley of Sd1 and Sd2L barley varieties, recombinant beta-amylase
native enzyme enriched fraction from powdered stems of Abrus precatorius using a three-phase partitioning method and a Doehlert design with 3 variables (ratio of crude extract/t-butanol, the ammonium sulfate saturation and pH). Data fitting in a second-order polynomial model and parameters optimization to enrich beta-amylase. Optimal conditions are: a ratio of crude extract/tert-butanol of 0.87 v/v, saturation in ammonium sulfate of 49.46% w/v, and a pH of 5.2. An activity recovery of 156.2% and a purification factor of 10.17 are found. Next steps are dialysis and lyophilisation
-
native enzyme from powdered stems by three-phase partitioning (TPP), method evaluation and optimization, and model validation, overview
-
native isozyme partially by chloroplast purification
native isozyme partially by chloroplast purification, recombinant mature isozyme TR-BAMY and recombinant mutant enzymes from Escherichia coli
partial
partial, 14.67fold
-
partially by preparation of enzyme extract and heat treatment at 70°C for 20 min
-
recombinant enzyme
-
recombinant mutant enzymes
-
recombinant wild-type and mutant enzymes
recombinant wild-type and mutant enzymes from Escherichia coli strain JM105
recombinant wild-type and mutant enzymes from Escherichia coli strain XL1-Blue
recombinant wild-type and mutants from Escherichia coli