3.2.1.2: beta-amylase
This is an abbreviated version!
For detailed information about beta-amylase, go to the full flat file.
Word Map on EC 3.2.1.2
-
3.2.1.2
-
starch
-
alpha-amylase
-
maltose
-
barley
-
potato
-
soybean
-
sweet
-
amylopectin
-
amylolytic
-
glucoamylase
-
endosperm
-
hordeum
-
amylases
-
pullulanase
-
debranching
-
dextrin
-
nutrition
-
maltotetraose
-
thermosulfurogenes
-
isoamylase
-
beta-limit
-
starch-degrading
-
amyloglucosidase
-
polymyxa
-
maltotriose
-
maltopentaose
-
granule-bound
-
alpha-cyclodextrin
-
extrachloroplastic
-
industry
-
sporamin
-
molecular biology
-
brewing
- 3.2.1.2
- starch
- alpha-amylase
- maltose
- barley
- potato
- soybean
-
sweet
- amylopectin
-
amylolytic
- glucoamylase
- endosperm
- hordeum
- amylases
- pullulanase
-
debranching
- dextrin
- nutrition
- maltotetraose
- thermosulfurogenes
- isoamylase
-
beta-limit
-
starch-degrading
- amyloglucosidase
- polymyxa
- maltotriose
- maltopentaose
-
granule-bound
- alpha-cyclodextrin
-
extrachloroplastic
- industry
-
sporamin
- molecular biology
- brewing
Reaction
Synonyms
(1-4)-alpha-D-glucan maltohydrolase, 1,4-alpha-D-glucan malto-hydrolase, 1,4-alpha-D-glucan maltohydrolase, 1-4-alpha-glucan maltohydrolase, alpha-1,4-glucan maltohydrolase, amylase, beta-, ARATH, BAM-1, BAM-2, BAM-3, BAM-5, BAM-6, BAM-7, BAM-8, BAM-9, BAM1, BAM3, BAM4, BCB, beta amylase, beta-amylase, beta-amylase 1, beta-amylase I, beta-amylase1, beta-amylase2, beta-amylase8, BMY, Bmy1, Bmy2, Cs-COR018, CT-BMY, glycogenase, More, PF0870, saccharogen amylase, saccharogenamylase, SBA, Sd1, Sd2H, Sd2L, spoII, TCMA, TR-BAMY, type I beta-amylase, type II beta-amylase
ECTree
3.2.1.2 beta-amylaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.2.1.2 - beta-amylase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.491
amylose
-
DP = 17, mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S
0.6
amylose
pH 7, 25°C, DPn: 16, S235A/Y249A/W449F/W495F quadruple mutant
0.0083
starch
-
pH 5, 40°C, soluble starch, wild-type Sd2H and V233A/L347S double mutant of Sd2L
additional information
additional information
-
Km: 1.25 mg/ml for starch
-
additional information
additional information
-
Km: 3 mg/ml for soluble starch
-
additional information
additional information
-
the Km-value for linear maltodextrins decreases with chain-lengths up to about 50 glucose units and with longer chain lengths it remains constant at about 0.14 mM
-
additional information
additional information
-
Km for soluble starch is 0.4%
-
additional information
additional information
-
5.9 mg/ml for soluble starch, light amylase. 6.8 mg/ml for soluble starch for soluble starch, heavy amylase
-
additional information
additional information
-
Km: 2.25 mg/ml for amylopectin for isoenzyme 2, Km: 1.65 mg/ml for amylopectin, isoenzyme 6
-
additional information
additional information
-
Km: 2.29 mg/ml for soluble starch at 60°C, 1.68 mg/ml for soluble starch at 75°C
-
additional information
additional information
-
Km: 1.67 mg/ml for soluble starch
-
additional information
additional information
beta-amylase from germinated barley has a higher substrate binding affinity for starch than enzyme from mature grain, removal of the four C-terminal glycine-rich repeats enhances the substrate binding affinity, kinetic parameters for several deletion mutants
-
additional information
additional information
-
beta-amylase from germinated barley has a higher substrate binding affinity for starch than enzyme from mature grain, removal of the four C-terminal glycine-rich repeats enhances the substrate binding affinity, kinetic parameters for several deletion mutants
-
additional information
additional information
binding parameters of wild-type and mutant enzymes to raw corn starch
-
additional information
additional information
-
binding parameters of wild-type and mutant enzymes to raw corn starch
-
additional information
additional information
kinetic parameters for wild-type and mutant SBA
-
additional information
additional information
-
kinetic parameters for wild-type and mutant SBA
-
additional information
additional information
-
Km for starch is 4.34 mg/ml at 60°C
-
additional information
additional information
-
the 3 allelic forms of beta-amylase Sd1, Sd2H and Sd2L exhibit different kinetic properties, an R115C mutation is responsible for this difference
-
additional information
additional information
-
kinetics, isothermal titration microcalorimetric method
-
additional information
additional information
kinetics, recombinant wild-type andmutant enzymes
-
additional information
additional information
-
kinetics, recombinant wild-type andmutant enzymes
-
additional information
additional information
-
13.6 mg/l, starch as substrate
-
additional information
additional information
-
Km and Vmax values are 79.37mg/ml and 5.13 U/ml, respectively, at 50°C, pH 6.0, Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics and Lineweaver-Burk plots, Km and Vmax values at 50°C are estimated to be 2.81 mg/ml and 10.62 U/min, respectively
-
additional information
additional information
Michaelis-Menten kinetics, modelling
-
