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3.2.1.2: beta-amylase

This is an abbreviated version!
For detailed information about beta-amylase, go to the full flat file.

Word Map on EC 3.2.1.2

Reaction

(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose
+
H2O
=
(alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose
 +
alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose

Synonyms

(1-4)-alpha-D-glucan maltohydrolase, 1,4-alpha-D-glucan malto-hydrolase, 1,4-alpha-D-glucan maltohydrolase, 1-4-alpha-glucan maltohydrolase, alpha-1,4-glucan maltohydrolase, amylase, beta-, ARATH, BAM-1, BAM-2, BAM-3, BAM-5, BAM-6, BAM-7, BAM-8, BAM-9, BAM1, BAM3, BAM4, BCB, beta amylase, beta-amylase, beta-amylase 1, beta-amylase I, beta-amylase1, beta-amylase2, beta-amylase8, BMY, Bmy1, Bmy2, Cs-COR018, CT-BMY, glycogenase, More, PF0870, saccharogen amylase, saccharogenamylase, SBA, Sd1, Sd2H, Sd2L, spoII, TCMA, TR-BAMY, type I beta-amylase, type II beta-amylase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.2 beta-amylase

Cloned

Cloned on EC 3.2.1.2 - beta-amylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 active enzyme forms are detected after expression in Escherichia coli, MW 60000 Da and MW 49000 Da. The 60000 Da enzyme form is 3000 Da larger than the mature enzyme from Bacillus circulans, suggesting that processing of the enzyme is different between the two species
-
a fusion gene that encodes a polypeptide of 1495 amino acids is constructed from the beta-amylase gene of Clostridium thermosulfurogenes and trehalose synthase gene of Thermus thermophilus. The fused gene is overexpressed in Escherichia coli, and a recombinant bifunctional fusion protein with beta-amylase at the N-terminal or C-terminal of trehalose synthase having both beta-amylase and trehalose synthase activities with an apparent molecular mass of 164000 Da is obtained
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
DNA sequence determination, construction of BMY8 knockout plants, and of BMY8 RNAi plants
expressed in Escherichia coli
-
expressed the gene in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli as a GST fusion protein
expression in Escherichia coli BL21 (DE3), pET 21a (+) vector
expression in Escherichia coli, the recombinant enzyme lacks 4 amino acids at positions 2-5 (Glu-Val-Asn-Val) when compared with barley beta-amylase
-
expression of a sevenfold mutant beta-amylase, M185L/S295A/I297V/S350P/S351P/Q352D/A376S
-
expression of wild-type and mutant enzymes in Escherichia coli strain JM105
expression of wild-type and mutant enzymes in Escherichia coli strain XL1-Blue
expression of wild-type and mutants in Escherichia coli strains JM109 and JM105
isozyme Bmy2 from cultivars Morex and Steptoe, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
-
isozyme CT-BAMY, DNA and amino acid sequence determination and analysis
isozyme TR-BAMY, DNA and amino acid sequence determination and analysis, functional expression of mature isozyme TR-BAMY and of mutant enzymes in Escherichia coli strains HB101 and BL21(DE3), transient expression of GFP-tagged isozyme TR-BAMY in Nicotiana tabacum protoplasts with exclusive association to the chloroplasts
phylogenetic analysis, expression of wild-type and mutant enzymes in Escherichia coli Rosetta2 cells, subcloning in strain JM109
recombinant enzyme expression in Bacillus choshinensis using the constitutive P2 promoter, derived from a cell-wall protein of the host bacterium, as the expression promoter for the beta-amylase
-
Sd1 and Sd2L, from developing grain, expression in Escherichia coli M15
the bamM gene from Bacillus megaterium DSM319 encoding an extracellular beta-amylase was isolated and completely sequenced
two of the 3 allelic forms of beta-amylase Sd1 and Sd2L are cloned and sequenced
-
two rice genes encoding the enzyme, genes BAC83770 and BAC83773, in tight linkage, expression analysis, the Nipponbare allele in gene BAC83773 highly suppresses the enzyme
Q6Z5B2, Q6Z5B7
wild-type and mutant enzymes, expression in Escherichia coli BL21(DE3)