3.2.1.194: ginsenosidase type IV
This is an abbreviated version!
For detailed information about ginsenosidase type IV, go to the full flat file.
Reaction
Synonyms
alpha-L-rhamnosidase, beta-glucosidase, beta-glycosidase, beta-xylosidase, BglG167b, bgp1, bgp1C, ginsenosidase type IV, ginsenosidase type IV-i-1, ginsenosidase type IV-i-2, ginsenosidase type IV-i-3, ginsenosidase type IV-ii, ginsenoside-transforming beta-glucosidase, More
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Substrates Products
Substrates Products on EC 3.2.1.194 - ginsenosidase type IV
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REACTION DIAGRAM
2-nitrophenyl-beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucopyranose
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4-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
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ginsenoside Rg2 + 2 H2O
protopanaxatriol + D-glucose + L-rhamnose
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ginsenoside Rh1 + H2O
protopanaxatriol aglycone + D-glucose
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ginsenoside F1 + H2O
protopanaxatriol + D-glucose
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ginsenoside Rg1 + D-xylose
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the enzyme hydrolyzes the 6-O-D-(1->2)-xyloside
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ginsenoside R1 + H2O
ginsenoside Rg1 + D-xylose
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the enzyme hydrolyzes the 6-O-L-D-(1->2)-xyloside
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ginsenoside R1 + H2O
ginsenoside Rg1 + D-xylose
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the enzyme hydrolyzes the 6-O-D-(1->2)-xyloside
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ginsenoside R1 + H2O
ginsenoside Rg1 + D-xylose
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the enzyme hydrolyzes the 6-O-L-D-(1->2)-xyloside
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ginsenoside Rg3 + 2 D-glucose
cf. EC 3.2.1.192
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ginsenoside Rb1 + H2O
ginsenoside Rg3 + 2 D-glucose
reaction of EC 3.2.1.192
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ginsenoside Rg3 + D-glucose
cf. EC 3.2.1.192
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ginsenoside Rd + H2O
ginsenoside Rg3 + D-glucose
reaction of EC 3.2.1.192
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ginsenoside Rg1 + L-rhamnose
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the enzyme hydrolyzes the 6-O-alpha-L-(1->2)-rhamnoside
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ginsenoside Re + H2O
ginsenoside Rg1 + L-rhamnose
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the enzyme hydrolyzes the 6-O-alpha-L-(1->2)-rhamnoside
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ginsenoside Re + H2O
ginsenoside Rg2 + L-rhamnose
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ginsenoside Rf + H2O
ginsenoside Rh1 + D-glucose
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the enzyme hydrolyzes 6-O-beta-D-(1->2)-glucoside
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ginsenoside Rf + H2O
ginsenoside Rh1 + D-glucose
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the enzyme hydrolyzes 6-O-beta-D-(1->2)-glucoside
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ginsenoside Rf + H2O
ginsenoside Rh1 + D-glucose
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ginsenoside F1 + D-glucose
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the enzyme weakly hydrolyzes 6-O-beta-D-glucoside
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ginsenoside Rg1 + H2O
ginsenoside F1 + D-glucose
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the enzyme weakly hydrolyzes 6-O-beta-D-glucoside
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ginsenoside Rg1 + H2O
ginsenoside Rh1 + D-glucose
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ginsenoside Rg2 + H2O
ginsenoside Rh1 + L-rhamnose
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the enzyme hydrolyzes 6-O-alpha-L-(1->2)-rhamnoside
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ginsenoside Rg2 + H2O
ginsenoside Rh1 + L-rhamnose
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protopanaxatriol + D-glucose
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the enzyme weakly hydrolyzes 6-O-beta-D-glucoside
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notoginsenoside R1 + H2O
ginsenoside Rg1 + D-xylose
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notoginsenoside R1 + H2O
notoginsenoside R2 + D-glucose
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1. This conversion has been conducted by beta-glycosidase from Aspergillus niger
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additional information
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ginsenosidase type IV can not hydrolyze the 3-O- or 20-O-glycosides of protopanaxadiol type ginsenosides (PPD), such as Rb1, Rb2, Rb3, Rc, and Rd
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additional information
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the ginsenosidase type IV can hydrolyze 6-O-multiglycosides including rhamnoside, xyloside and glucoside of protopanaxatriol (PPT) type ginsenosides, but cannot hydrolyze the glycosides of protopanaxadiol (PPD) type ginsenoside
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-ii enzymes convert ginsenosides Rg1 and Rh1, containing only the inner glucose without the outer sugar at C-6, are converted to ginsenosides F1 and aglycone protopanaxatriol (APPT), respectively, by hydrolyzing the inner glucose linked to C-6 but not the glucose linked to C-20 in the PPT-type ginsenosides with the hydrolysis pattern of F. Ginsenosidase type IV-ii enzymes also hydrolyze the outer glucose of ginsenoside Rf, which has the outer and inner glucose at C-6 with the hydrolysis pattern of E-1; the outer rhamnose of ginsenosides Re and Rg2, which have the outer rhamnose and inner glucose at C-6 with the hydrolysis attern of E-2, and the outer xylose of notoginsenosides R1 and R2, which contain the outer xylose and inner glucose at C-6 with the hydrolysis pattern of E-3
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additional information
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ginsenosidase type IV can not hydrolyze the 3-O- or 20-O-glycosides of protopanaxadiol type ginsenosides (PPD), such as Rb1, Rb2, Rb3, Rc, and Rd
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additional information
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the ginsenosidase type IV can hydrolyze 6-O-multiglycosides including rhamnoside, xyloside and glucoside of protopanaxatriol (PPT) type ginsenosides, but cannot hydrolyze the glycosides of protopanaxadiol (PPD) type ginsenoside
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additional information
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the specific activity of beta-glucosidase from Clavibacter michiganensis for the PPD-type ginsenosides as substrates followed the order Rb2 > Rd > Rc > Rg3 > Rb1 > compound O (C-O) > compound Mc1 (C-Mc1) > GypXVII > F2 > Rh2 > gypenside LXXV (GypLXXV) and the products of which are C-O, F2, C-Mc1, Rh2, GypXVII, compound Y (C-Y), compound Mc (C-Mc), GypLXXV, compound K (C-K), APPD, and C-K, respectively. The specific activity of beta-glucosidase from Clavibacter michiganensis for the PPT-type ginsenosides as substrates followed the order Rf > Rh1 > Rg1 and the products of which are Rh1, APPT, and F1, respectively
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additional information
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recombinant beta-glucosidase from Clavibacter michiganensis specifically hydrolyzes the outer and inner glucose linked to the C-3 position in protopanaxadiol (PPD)-type ginsenosides (EC 3.2.1.191) and the C-6 position in protopanaxatriol (PPT)-type ginsenosides (EC 3.2.1.194) except for the hydrolysis of gypenoside LXXV (GypLXXV). The enzyme converts gypenoside XVII (GypXVII) to GypLXXV by hydrolyzing the inner glucose linked to the C-3 position
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additional information
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the specific activity of beta-glucosidase from Clavibacter michiganensis for the PPD-type ginsenosides as substrates followed the order Rb2 > Rd > Rc > Rg3 > Rb1 > compound O (C-O) > compound Mc1 (C-Mc1) > GypXVII > F2 > Rh2 > gypenside LXXV (GypLXXV) and the products of which are C-O, F2, C-Mc1, Rh2, GypXVII, compound Y (C-Y), compound Mc (C-Mc), GypLXXV, compound K (C-K), APPD, and C-K, respectively. The specific activity of beta-glucosidase from Clavibacter michiganensis for the PPT-type ginsenosides as substrates followed the order Rf > Rh1 > Rg1 and the products of which are Rh1, APPT, and F1, respectively
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additional information
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recombinant beta-glucosidase from Clavibacter michiganensis specifically hydrolyzes the outer and inner glucose linked to the C-3 position in protopanaxadiol (PPD)-type ginsenosides (EC 3.2.1.191) and the C-6 position in protopanaxatriol (PPT)-type ginsenosides (EC 3.2.1.194) except for the hydrolysis of gypenoside LXXV (GypLXXV). The enzyme converts gypenoside XVII (GypXVII) to GypLXXV by hydrolyzing the inner glucose linked to the C-3 position
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides
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additional information
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substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides, overview. The purified recombinant enzyme specifically hydrolyzes the glucopyranosides at the C-20 position in protopanaxadiol (PPD)-type ginsenosides and hydrolyzes the glucopyranoside at the C-6 or C-20 position in protopanaxatriol (PPT)-type ginsenosides. The enzyme is a beta-glucosidase with xylopyranoside-hydrolyzing activity that is active with 4-nitrophenyl-beta-D-xylopyranoside and 2-nitrophenyl-beta-D-xylopyranoside. The specific activity for PPD-type ginsenosides as substrates is in the order gypenoside XVII > gypenoside LXXV > Rb1 > F2 > compound K > Rd, and the products are F2, compound K, Rd, Rh2, APPD, and Rg3, respectively. The specific activity for PPT-type ginsenosides as substrates follows the order F1 > Re > Rg1 > notoginsenoside R1 > Rh1 and the products of which are APPT, Rg2, Rh1, notoginsenoside R2, and APPT, respectively. No activity with ginsenosides Rb2, Rc, Compound Mc, Compound Mc-1, Compound O, and Compound Y, as well as with notoginsenoside R2 and ginsenoside Rg2. No activity is found for 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranose, 4-nitrophenyl-alpha-L-arabinopyranose, or 4-nitrophenyl-alpha-L-rhamnopyranoside
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additional information
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substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides, overview. The purified recombinant enzyme specifically hydrolyzes the glucopyranosides at the C-20 position in protopanaxadiol (PPD)-type ginsenosides and hydrolyzes the glucopyranoside at the C-6 or C-20 position in protopanaxatriol (PPT)-type ginsenosides. The enzyme is a beta-glucosidase with xylopyranoside-hydrolyzing activity that is active with 4-nitrophenyl-beta-D-xylopyranoside and 2-nitrophenyl-beta-D-xylopyranoside. The specific activity for PPD-type ginsenosides as substrates is in the order gypenoside XVII > gypenoside LXXV > Rb1 > F2 > compound K > Rd, and the products are F2, compound K, Rd, Rh2, APPD, and Rg3, respectively. The specific activity for PPT-type ginsenosides as substrates follows the order F1 > Re > Rg1 > notoginsenoside R1 > Rh1 and the products of which are APPT, Rg2, Rh1, notoginsenoside R2, and APPT, respectively. No activity with ginsenosides Rb2, Rc, Compound Mc, Compound Mc-1, Compound O, and Compound Y, as well as with notoginsenoside R2 and ginsenoside Rg2. No activity is found for 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranose, 4-nitrophenyl-alpha-L-arabinopyranose, or 4-nitrophenyl-alpha-L-rhamnopyranoside
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additional information
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substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides, overview. The purified recombinant enzyme specifically hydrolyzes the glucopyranosides at the C-20 position in protopanaxadiol (PPD)-type ginsenosides and hydrolyzes the glucopyranoside at the C-6 or C-20 position in protopanaxatriol (PPT)-type ginsenosides. The enzyme is a beta-glucosidase with xylopyranoside-hydrolyzing activity that is active with 4-nitrophenyl-beta-D-xylopyranoside and 2-nitrophenyl-beta-D-xylopyranoside. The specific activity for PPD-type ginsenosides as substrates is in the order gypenoside XVII > gypenoside LXXV > Rb1 > F2 > compound K > Rd, and the products are F2, compound K, Rd, Rh2, APPD, and Rg3, respectively. The specific activity for PPT-type ginsenosides as substrates follows the order F1 > Re > Rg1 > notoginsenoside R1 > Rh1 and the products of which are APPT, Rg2, Rh1, notoginsenoside R2, and APPT, respectively. No activity with ginsenosides Rb2, Rc, Compound Mc, Compound Mc-1, Compound O, and Compound Y, as well as with notoginsenoside R2 and ginsenoside Rg2. No activity is found for 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranose, 4-nitrophenyl-alpha-L-arabinopyranose, or 4-nitrophenyl-alpha-L-rhamnopyranoside
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additional information
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the recombinant beta-glucosidase enzyme (bgp1) hydrolyzes all glucose moieties attached to the C-20 position of the ginsenosides Rg1, Re, Rb1, Rd, and F1. The products include pharmacologically active minor ginsenosides Rh1, Rg2, Rg3, F1, and protopanaxatriol. TLC and HPLC analysis of ginsenoside transformation by enzyme bgp1, overview
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additional information
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enzyme can hydrolyze various ginsenosides, i.e. Rb1, Rb2, Rc, Rd, Rg3(S), F2, Re, Rg1, GypXVII, through various cleavage pathways, substrate specificity, overview. BglG167b cannot hydrolyze the attached sugars at C-6, but glucose moieties at C-3
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2. This conversion was performed using b-glycosidase from Pyrococcus furiosus
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1. This conversion has been conducted by beta-glycosidase from Pyrococcus furiosus
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2. This conversion is performed using beta-glycosidase from Pyrococcus furiosus
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides. beta-Gglycosidase from Pyrococcus furiosus shows broad substrate specificity that hydrolyzes the outer residue glucose and rhamnose and xylose at C-6
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides
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additional information
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ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides. beta-Xylosidase from Thermoanaerobacterium thermosaccharolyticum converts notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1 by selectively hydrolyzing only the outer xylose at C-6
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