3.2.1.192: ginsenoside Rb1 beta-glucosidase

This is an abbreviated version!
For detailed information about ginsenoside Rb1 beta-glucosidase, go to the full flat file.

Reaction

+ 2 H2O =

+ 2 D-glucopyranose

Synonyms

BGL3T, bgp1, bgp1C, GH3 beta-glucosidase, ginsenoside Rb1-converting enzyme, More, Theth_1706, Tpebgl3, Tpet_0898, Tt-BGL

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.192 ginsenoside Rb1 beta-glucosidase

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Results	in table
4	Activating Compound
3	AA Sequence
4	Application
4	Cloned(Commentary)
2	Expression
7	General Information
2	General Stability
15	Inhibitors
4	kcat/KM [mM/s]
8	KM Value [mM]
2	Localization
4	Metals/Ions
3	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
23	Organism
2	Pathway
10	pH Optimum
6	pH Range
5	pH Stability
1	pI Value
4	Purification (Commentary)
6	Reaction
19	Reference
5	Source Tissue
10	Specific Activity [micromol/min/mg]
76	Substrates and Products (Substrate)
9	Subunits
33	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
6	Temperature Range [°C]
8	Temperature Stability [°C]
4	Turnover Number [1/s]

Systematic Name

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Systematic Name on EC 3.2.1.192 - ginsenoside Rb1 beta-glucosidase

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ginsenoside Rb1 glucohydrolase

Ginsenosidases catalyse the hydrolysis of glycosyl moieties attached to the C-3, C-6 or C-20 position of ginsenosides. They are specific with respect to the nature of the glycosidic linkage, the position and the order in which the linkages are cleaved. Ginsenoside Rb1 beta-glucosidase specifically and sequentially hydrolyses the 20-[beta-D-glucopyranosyl-(1->6)-beta-D glucopyranosyloxy] residues attached to position 20 by first hydrolysing the (1->6)-glucosidic bond to generate ginsenoside Rd as an intermediate, followed by hydrolysis of the remaining 20-O-beta-D-glucosidic bond.