3.2.1.180: unsaturated chondroitin disaccharide hydrolase

This is an abbreviated version!
For detailed information about unsaturated chondroitin disaccharide hydrolase, go to the full flat file.

Reaction

Synonyms

Phep_2238, Phep_2649, Phep_2830, SagUGL, SpnUGL, SpyUGL, UGL, unsaturated glucuronyl hydrolase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.180 unsaturated chondroitin disaccharide hydrolase

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Results	in table
277	AA Sequence
11	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
5	General Information
2	IC50 Value
24	Inhibitors
29	kcat/KM [mM/s]
35	KM Value [mM]
6	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
15	Organism
4	Pathway
1	PDB ID
13	pH Optimum
22	Protein Variants
8	Purification (Commentary)
1	Reaction
13	Reference
7	Specific Activity [micromol/min/mg]
96	Substrates and Products (Substrate)
11	Subunits
30	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
3	Temperature Stability [°C]
33	Turnover Number [1/s]

KM Value

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KM Value on EC 3.2.1.180 - unsaturated chondroitin disaccharide hydrolase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.58

2,4,6-trichlorophenyl 4-deoxy-alpha-L-threo-hex-4-enopyranosiduronic acid

Clostridium perfringens

-

pH 6.6, 37°C

731656

0.16

2,4-dinitrophenyl 4-deoxy-alpha-L-threo-hex-4-enopyranosiduronic acid

Clostridium perfringens

-

pH 6.6, 37°C

731656

0.59

2,5-dinitrophenyl 4-deoxy-alpha-L-threo-hex-4-enopyranosiduronic acid

Clostridium perfringens

-

pH 6.6, 37°C

731656

4.5

2-(acetylamino)-2-deoxy-3-O-(4-deoxy-alpha-L-threo-hex-4-enopyranuronosyl)-4-O-sulfo-D-galactopyranose

Clostridium perfringens

-

pH 6.6, 37°C

731656

3.7

2-(acetylamino)-2-deoxy-3-O-(4-deoxy-alpha-L-threo-hex-4-enopyranuronosyl)-6-O-sulfo-D-galactopyranose

Clostridium perfringens

-

pH 6.6, 37°C

731656

3

2-(acetylamino)-2-deoxy-3-O-(4-deoxy-alpha-L-threo-hex-4-enopyranuronosyl)-D-galactopyranose

Clostridium perfringens

-

pH 6.6, 37°C

731656

1.2

2-deoxy-4-O-(4-deoxy-alpha-L-threo-hex-4-enopyranuronosyl)-2-(sulfoamino)-D-glucopyranose

Clostridium perfringens

-

pH 6.6, 37°C

731656

0.88

3-nitrophenyl 4-deoxy-alpha-L-threo-hex-4-enopyranosiduronic acid

Clostridium perfringens

-

pH 6.6, 37°C

731656

0.84

4-chlorophenyl 4-deoxy-alpha-L-threo-hex-4-enopyranosiduronic acid

Clostridium perfringens

-

pH 6.6, 37°C

731656

0.26

4-nitrophenyl 4-deoxy-alpha-L-threo-hex-4-enopyranosiduronic acid

Clostridium perfringens

-

pH 6.6, 37°C

731656

0.88

4-tert-butylphenyl 4-deoxy-alpha-L-threo-hex-4-enopyranosiduronic acid

Clostridium perfringens

-

pH 6.6, 37°C

731656

9

benzyl 4-deoxy-alpha-L-threo-hex-4-enopyranosiduronic acid

Clostridium perfringens

-

pH 6.6, 37°C

731656

0.371 - 1.4

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc

4 entries

0.1 - 2.36

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S

8 entries

0.86 - 5.2

DELTAGlcUA-beta-(1->4)-GlcN2S

6 entries

0.029 - 1.4

DELTAGlcUA-beta-(1->4)-GlcNAc

2 entries

3.2

phenyl 4-deoxy-alpha-L-threo-hex-4-enopyranosiduronic acid

Clostridium perfringens

-

pH 6.6, 37°C

731656

additional information

additional information

2 entries

-

0.371

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc

Streptococcus agalactiae

Q8E372

pH 7.5, 30°C, mutant enzyme K370I

709164

0.763

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc

Streptococcus agalactiae

Q8E372

pH 7.5, 30°C, mutant enzyme S365H

709164

1.27

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc

Streptococcus agalactiae

Q8E372

pH 7.5, 30°C, wild-type enzyme

709164

1.4

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc

Streptococcus agalactiae

Q8E372

pH 7.5, 30°C, mutant enzyme S368G

709164

0.1

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S

Streptococcus agalactiae

Q8E372

pH 7.5, 30°C, wild-type enzyme

709164

0.18

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S

Streptococcus pneumoniae

Q8DR77

pH 7.5, 30°C. wild-type enzyme

709026

0.191

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S

Streptococcus agalactiae

Q8E372

pH 7.5, 30°C, mutant enzyme S368G

709164

0.39

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S

Streptococcus pyogenes

Q9A0T3

pH 7.5, 30°C. wild-type enzyme

709026

0.54

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S

Streptococcus agalactiae

Q8E372

pH 7.5, 30°C. wild-type enzyme

709026

1.47

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S

Streptococcus agalactiae

Q8E372

pH 7.5, 30°C, mutant enzyme K370I

709164

1.75

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S

Streptococcus agalactiae

Q8E372

pH 7.5, 30°C, mutant enzyme T235A

709164

2.36

beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S

Streptococcus agalactiae

Q8E372

pH 7.5, 30°C, mutant enzyme S365H

709164

0.86

DELTAGlcUA-beta-(1->4)-GlcN2S

Streptococcus agalactiae

-

pH 7.5, 30°C, recombinant mutant S370A

732164

1.9

DELTAGlcUA-beta-(1->4)-GlcN2S

Streptococcus agalactiae

-

pH 7.5, 30°C, recombinant wild-type enzyme

732164

3

DELTAGlcUA-beta-(1->4)-GlcN2S

Streptococcus agalactiae

-

pH 7.5, 30°C, recombinant mutant S368A

732164

3.4

DELTAGlcUA-beta-(1->4)-GlcN2S

Streptococcus agalactiae

-

pH 7.5, 30°C, recombinant mutant S365A

732164

3.4

DELTAGlcUA-beta-(1->4)-GlcN2S

Streptococcus agalactiae

-

pH 7.5, 30°C, recombinant mutant S368G

732164

5.2

DELTAGlcUA-beta-(1->4)-GlcN2S

Streptococcus agalactiae

-

pH 7.5, 30°C, recombinant mutant S365G

732164

0.029

DELTAGlcUA-beta-(1->4)-GlcNAc

Pedobacter heparinus

C6XY24, C6Y0D8, C6Y1N7

pH 7.5, 30°C, recombinant wild-type enzyme

732164

1.4

DELTAGlcUA-beta-(1->4)-GlcNAc

Pedobacter heparinus

C6XY24, C6Y0D8, C6Y1N7

pH 7.5, 30°C, recombinant mutant F164A

732164

additional information

additional information

Clostridium perfringens

-

Michaelis-Menten kinetic parameters for the cleavage of a variety of unsaturated disaccharides of glycosaminoglycan origin, overview

731656

-

additional information

additional information

Bacillus sp. GL1

-

free-energy profiles of the catalytic reaction, overview

754479

-